Recombinant Human IL-6 R alpha Protein, CF

(26 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. Saito, T. et al. (1991) J. Immunol. 147:168. The ED50 for this effect is 5-15 ng/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived Leu20-Asp358
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu20
  • Predicted Molecular Mass
    38 kDa
  • SDS-PAGE
    45-50 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
227-SR/CF
 
227-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-6 R alpha (Catalog # 227-SR/CF) enhances the IL-6 activity on the M1 mouse myeloid leukemia cell line. The ED50 for this effect is 5-15 ng/mL.
1 μg/lane of Recombinant Human IL-6 R alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 45‑50 kDa.
Background: IL-6 R alpha

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R and gp130 have been cloned, sequenced, and expressed (1 - 4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 alpha R cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

  • References:
    1. Yamasaki et al. (1988) Science 241:825.
    2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
    3. Hibi et al. (1990) Cell 63:1149.
    4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
    5. Novick et al., (1989) J. Exp. Med. 170:1409.
    6. Yasukawa et al. (1990) J. Biochem. 108:673.
    7. Saito et al. (1991) J. Immunology 147:168.
  • Long Name:
    Interleukin 6 Receptor alpha
  • Entrez Gene IDs:
    3570 (Human); 16194 (Mouse)
  • Alternate Names:
    CD126; IL-6 R alpha; IL6R alpha; IL6R; IL-6Ra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. SorLA in interleukin-6 signaling and turnover
    Authors: JV Larsen, CM Petersen
    Mol. Cell. Biol, 2017;0(0):.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  2. COPD monocytes demonstrate impaired migratory ability
    Authors: AK Ravi, J Plumb, R Gaskell, S Mason, CS Broome, G Booth, M Catley, J Vestbo, D Singh
    Respir. Res., 2017;18(1):90.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Structure-based virtual screening and characterization of a novel IL-6 antagonistic compound from synthetic compound database
    Drug Des Devel Ther, 2016;10(0):4091-4100.
    Species: Human
    Sample Type: Recombinant Protein
    Application: EnzAct
  4. A Novel IL6 Antibody Sensitizes Multiple Tumor Types to Chemotherapy Including Trastuzumab-Resistant Tumors.
    Authors: Zhong H, Davis A, Ouzounova M, Carrasco R, Chen C, Breen S, Chang Y, Huang J, Liu Z, Yao Y, Hurt E, Moisan J, Fung M, Tice D, Clouthier S, Xiao Z, Wicha M, Korkaya H, Hollingsworth R
    Cancer Res, 2016;76(2):480-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. STAT3-induced WDR1 overexpression promotes breast cancer cell migration
    Cell Signal, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Interleukin 6 receptor is an independent prognostic factor and a potential therapeutic target of ovarian cancer.
    Authors: Isobe, Aki, Sawada, Kenjiro, Kinose, Yasuto, Ohyagi-Hara, Chifumi, Nakatsuka, Erika, Makino, Hiroshi, Ogura, Tomonori, Mizuno, Tomoko, Suzuki, Noriko, Morii, Eiichi, Nakamura, Koji, Sawada, Ikuko, Toda, Aska, Hashimoto, Kae, Mabuchi, Seiji, Ohta, Tsuyoshi, Morishige, Ken-ichi, Kurachi, Hirohisa, Kimura, Tadashi
    PLoS ONE, 2015;10(2):e0118080.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling.
    Authors: Sobotta M, Liou W, Stocker S, Talwar D, Oehler M, Ruppert T, Scharf A, Dick T
    Nat Chem Biol, 2015;11(1):64-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. RelB/p50 complexes regulate cytokine-induced YKL-40 expression.
    Authors: Bhardwaj R, Yester J, Singh S, Biswas D, Surace M, Waters M, Hauser K, Yao Z, Boyce B, Kordula T
    J Immunol, 2015;194(6):2862-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Discovery and characterization of olokizumab: a humanized antibody targeting interleukin-6 and neutralizing gp130-signaling.
    Authors: Shaw S, Bourne T, Meier C, Carrington B, Gelinas R, Henry A, Popplewell A, Adams R, Baker T, Rapecki S, Marshall D, Moore A, Neale H, Lawson A
    MAbs, 2014;6(3):774-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling.
    Authors: Csaszar, Elizabet, Wang, Weijia, Usenko, Tatiana, Qiao, Wenlian, Delaney, Colleen, Bernstein, Irwin D, Zandstra, Peter W
    Blood, 2014;123(5):650-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Protein tyrosine phosphatase PTPN9 regulates erythroid cell development through STAT3 dephosphorylation in zebrafish.
    Authors: Bu Y, Su F, Wang X, Gao H, Lei L, Chang N, Wu Q, Hu K, Zhu X, Chang Z, Meng K, Xiong J
    J Cell Sci, 2014;127(0):2761-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Interleukin-6 (IL-6) trans signaling drives a STAT3-dependent pathway that leads to hyperactive transforming growth factor-beta (TGF-beta) signaling promoting SMAD3 activation and fibrosis via Gremlin protein.
    Authors: O'Reilly S, Ciechomska M, Cant R, van Laar J
    J Biol Chem, 2014;289(14):9952-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Targeting protein kinase CK2 suppresses prosurvival signaling pathways and growth of glioblastoma.
    Authors: Zheng Y, McFarland B, Drygin D, Yu H, Bellis S, Kim H, Bredel M, Benveniste E
    Clin Cancer Res, 2013;19(23):6484-94.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  14. CTR9, a component of PAF complex, controls elongation block at the c-Fos locus via signal-dependent regulation of chromatin-bound NELF dissociation.
    Authors: Yoo H, Seo J, Yoo J
    PLoS ONE, 2013;8(4):e61055.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. NF-kappaB-induced IL-6 ensures STAT3 activation and tumor aggressiveness in glioblastoma.
    Authors: McFarland B, Hong S, Rajbhandari R, Twitty G, Gray G, Yu H, Benveniste E, Nozell S
    PLoS ONE, 2013;8(11):e78728.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  16. Soluble interleukin-6 receptor induces motor stereotypies and co-localizes with gp130 in regions linked to cortico-striato-thalamo-cortical circuits.
    Authors: Patel A, Zhu Y, Kuzhikandathil E, Banks W, Siegel A, Zalcman S
    PLoS ONE, 2012;7(7):e41623.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  17. Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis.
    Authors: Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G
    Blood, 2010;116(16):3099-107.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Blocking
  18. Interleukin-7 stimulates secretion of S100A4 by activating the JAK/STAT signaling pathway in human articular chondrocytes.
    Authors: Yammani RR, Long D, Loeser RF
    Arthritis Rheum., 2009;60(3):792-800.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009;69(1):329-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus.
    Authors: Umehara A, Nishioka M, Obata T
    Clin. Biochem., 2009;42(13):1468-75.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  21. Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
    Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(38):14692-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle.
    Authors: Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(1):L199-206.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. CD14 is an acute-phase protein.
    Authors: Bas S, Gauthier BR, Spenato U, Stingelin S, Gabay C
    J. Immunol., 2004;172(7):4470-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  25. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  26. Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
    Authors: Erices A, Conget P, Rojas C, Minguell JJ
    Exp. Cell Res., 2002;280(1):24-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 26 Citations

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