Recombinant Human Integrin alpha V beta 1 Protein, CF

Catalog # Availability Size / Price Qty
6579-AVB-050
6579-AVB-01M
Recombinant Human Integrin alpha V beta 1 Protein Binding Activity
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Product Details
Citations (2)
FAQs
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Recombinant Human Integrin alpha V beta 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Fibronectin (Catalog # 4305-FNB) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Integrin alpha V beta 1 binds with an ED50 of 0.08-0.48 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 1 protein
Human Integrin alpha V
(Phe31-Val992)
Accession # NP_002201
GGGSGGGSGGGS Acidic Tail HHHHHH
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556
GGGSGGGSGGGS Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Phe31 ( alpha V subunit) & Gln21 ( beta 1 subunit, inferred from enzymatic pyroglutamate treatment revealing Thr22)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
115 kDa ( alpha V subunit) & 86 kDa ( beta 1 subunit)
SDS-PAGE
120-170 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6579-AVB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Data Image

Binding Activity Recombinant Human Integrin alpha V beta 1 Protein Binding Activity View Larger

When Recombinant Human Fibronectin (Catalog # 4305-FNB) is coated at 2 µg/mL Recombinant Human Integrin aV beta 1 (Catalog # 6579-AVB) binds with an ED50 of 0.08-0.48 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 1

Integrin alpha V beta 1 is one of five alpha V and twelve beta 1 containing Integrin family adhesion receptor heterodimers (1‑3). The non-covalent heterodimer of 170 kDa alpha V and 130 kDa beta 1/CD29 is present on cells that express both subunits, and dimer formation is dependant on the availability of the individual subunits (4). Since the alpha V and beta 1 subunits are widely expressed, the alpha V beta 1 heterodimer potentially forms in many cell types. The 962 aa human alpha V extracellular domain (ECD) shares 92‑95% aa sequence identity with mouse, rat and bovine alpha V, while the 708 aa human beta 1 ECD shares 92‑96% aa sequence identity with mouse, rat, bovine and feline  beta 1. The alpha V ECD contains an N‑terminal beta ‑propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1). alpha V integrins bind ligands that contain an RGD motif, including vitronectin, fibronectin and osteopontin (4‑9). The relatively weak binding affinity of alpha V beta 1 to vitronectin and fibronectin is thought to facilitate its activity in cyclic binding and release during cell migration (4, 5). In oligodendrocytes, astrocytes and pancreatic beta cells, alpha V beta 1 is expressed early in differentiation when cells are migrating and is down‑regulated when differentiation is complete (5‑7). alpha V beta 1 has also been found to be a receptor for angiopoietin-2 in Tie2-deficient glioma cells, and to mediate cell entry of viruses such as foot‑and‑mouth disease virus and human metaneumovirus (10‑12).

References
  1. Hynes, R. O. (2002) Cell 110:673.
  2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
  4. Koistinen, P. and J. Heino (2002) J. Biol. Chem. 277:24835.
  5. Kaido, T. et al. (2004) J. Biol. Chem. 279:17731.
  6. Milner, R. et al. (1996) J. Neurosci. 16:7240.
  7. Milner, R. et al. (2001) Mol. Cell. Neurosci. 18:108.
  8. Koivisto, L. et al. (2000) Exp. Cell Res. 255:10.
  9. Hu, D.D. et al. (1995) J. Biol. Chem. 270:26232.
  10. Hu, B. et al. (2006) Cancer Res. 66:775.
  11. Jackson, T. et al. (2002) J. Virol. 76:935.
  12. Cseke, G. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1566.
Entrez Gene IDs
3685 (Human)
Alternate Names
CD51; Integrin alpha V beta 1; integrin subunit alpha V; MSK8; VNRA; VTNR

Citations for Recombinant Human Integrin alpha V beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention.
    Authors: Stampolidis P, Ullrich A, Iacobelli S
    Oncogene, 2015;34(1):39-52.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
    Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
    PLoS ONE, 2013;8(11):e81165.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Binding Assay

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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