Recombinant Human Lgr6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
8470-GP-050
Recombinant Human Lgr6 Fc Chimera Protein, CF Bioactivity
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human Lgr6 Fc Chimera Protein, CF Summary

Purity
>80%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived human Lgr6 protein
Human Lgr6
(Ala25-Ala567)
Accession # Q9HBX8
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
87 kDa
SDS-PAGE
90-108 kDa, reducing conditions

Product Datasheets

8470-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Lgr6 Fc Chimera Protein, CF Bioactivity View Larger

Recombinant Human Lgr6 induces Topflashreporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is2.5-12.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Lgr6

Lgr6 (leucine‑rich repeat G protein‑coupled receptor 6) is an approximately 100 kDa 7‑transmembrane glycoprotein in the Lgr family of cell surface receptors. Lgrs has been identified as a marker for specific types of stem cells in tissues that are subject to constant self-renewal such as skin, gastrointestinal tract, and lungs (1, 2). The 544 amino acid (aa) N-terminal extracellular domain (ECD) of mature human Lgr6 contains 15 LRRs flanked by N-terminal and C-and C-terminal LRR domains. The ECD is followed by a 7-TM region and a 137 aa C-terminal cytoplasmic domain (3). Within the N-terminal ECD, human Lgr6 shares 92% and 93% aa sequence identity with mouse and rat Lgr6, respectively. Alternative splicing generates an additional isoform with a substitution for the LRR N-terminal domain and a deletion of LRR3-6. Lgr6 is expressed on hair follicle stem cells that give rise to sebaceous glands, interfollicular epidermis, and hair follicles (4-6). It is also expressed on alveolar stem cells that differentiate into lung epithelium (7). It is often up-regulated in human gastric cancers (8). Lgr6 binds to R‑Spondins 1-4 and can enhance Wnt/beta-Catenin signaling through phosphorylation of the Wnt co-receptor LRP6 (9, 10).

References
  1. de Lau, W. et al. (2014) Genes Dev. 28:305.
  2. Leushacke, M. and N. Barker (2012) Oncogene 31:3009.
  3. Hsu, S.Y. et al. (2000) Mol. Endocrinol. 14:1257.
  4. Snippert, H.J. et al. (2010) Science 327:1385.
  5. Liao, X.-H. and H. Nguyen (2014) Exp. Dermatol. 23:195.
  6. Nath, M. et al. (2011) Cell Tissue Res. 344:435.
  7. Oeztuerk-Winder, F. et al. (2012) EMBO J. 31:3431.
  8. Steffen, J.S. et al. (2012) Virchows Arch. 461:355.
  9. Gong, X. et al. (2012) PLoS ONE 7:e37137.
  10. de Lau, W. et al. (2011) Nature 476:293.
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 6
Entrez Gene IDs
59352 (Human); 329252 (Mouse); 498233 (Rat)
Alternate Names
FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Primary Antibodies

Human IgG1 Antibody

Reconstitution Buffers

Reviews for Recombinant Human Lgr6 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Lgr6 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human Lgr6 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review