Recombinant Human Lgr6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
Lgr6 Enhances Wnt Signaling.
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Product Details
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Recombinant Human Lgr6 Fc Chimera Protein, CF Summary

Product Specifications

>80%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Chinese Hamster Ovary cell line, CHO-derived human Lgr6 protein
Human Lgr6
Accession # Q9HBX8
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
87 kDa
90-108 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Lgr6 Enhances Wnt Signaling. View Larger

Recombinant Human Lgr6 induces Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Reconstitution Calculator

Reconstitution Calculator

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Background: Lgr6

Lgr6 (leucine‑rich repeat G protein‑coupled receptor 6) is an approximately 100 kDa 7‑transmembrane glycoprotein in the Lgr family of cell surface receptors. Lgrs has been identified as a marker for specific types of stem cells in tissues that are subject to constant self-renewal such as skin, gastrointestinal tract, and lungs (1, 2). The 544 amino acid (aa) N-terminal extracellular domain (ECD) of mature human Lgr6 contains 15 LRRs flanked by N-terminal and C-and C-terminal LRR domains. The ECD is followed by a 7-TM region and a 137 aa C-terminal cytoplasmic domain (3). Within the N-terminal ECD, human Lgr6 shares 92% and 93% aa sequence identity with mouse and rat Lgr6, respectively. Alternative splicing generates an additional isoform with a substitution for the LRR N-terminal domain and a deletion of LRR3-6. Lgr6 is expressed on hair follicle stem cells that give rise to sebaceous glands, interfollicular epidermis, and hair follicles (4-6). It is also expressed on alveolar stem cells that differentiate into lung epithelium (7). It is often up-regulated in human gastric cancers (8). Lgr6 binds to R‑Spondins 1-4 and can enhance Wnt/beta-Catenin signaling through phosphorylation of the Wnt co-receptor LRP6 (9, 10).

  1. de Lau, W. et al. (2014) Genes Dev. 28:305.
  2. Leushacke, M. and N. Barker (2012) Oncogene 31:3009.
  3. Hsu, S.Y. et al. (2000) Mol. Endocrinol. 14:1257.
  4. Snippert, H.J. et al. (2010) Science 327:1385.
  5. Liao, X.-H. and H. Nguyen (2014) Exp. Dermatol. 23:195.
  6. Nath, M. et al. (2011) Cell Tissue Res. 344:435.
  7. Oeztuerk-Winder, F. et al. (2012) EMBO J. 31:3431.
  8. Steffen, J.S. et al. (2012) Virchows Arch. 461:355.
  9. Gong, X. et al. (2012) PLoS ONE 7:e37137.
  10. de Lau, W. et al. (2011) Nature 476:293.
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 6
Entrez Gene IDs
59352 (Human); 329252 (Mouse); 498233 (Rat)
Alternate Names
FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631


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