Recombinant Human Lgr6 Fc Chimera Protein, CF

  
  • Purity
    >80%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human Lgr6
    (Ala25-Ala567)
    Accession # Q9HBX8
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala25
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    87 kDa
  • SDS-PAGE
    90-108 kDa, reducing conditions
8470-GP
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Lgr6 Enhances Wnt Signaling. 
Recombinant Human Lgr6 induces Topflashreporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is2.5-12.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).
Background: Lgr6
Lgr6 (leucine‑rich repeat G protein‑coupled receptor 6) is an approximately 100 kDa 7‑transmembrane glycoprotein in the Lgr family of cell surface receptors. Lgrs has been identified as a marker for specific types of stem cells in tissues that are subject to constant self-renewal such as skin, gastrointestinal tract, and lungs (1, 2). The 544 amino acid (aa) N-terminal extracellular domain (ECD) of mature human Lgr6 contains 15 LRRs flanked by N-terminal and C-and C-terminal LRR domains. The ECD is followed by a 7-TM region and a 137 aa C-terminal cytoplasmic domain (3). Within the N-terminal ECD, human Lgr6 shares 92% and 93% aa sequence identity with mouse and rat Lgr6, respectively. Alternative splicing generates an additional isoform with a substitution for the LRR N-terminal domain and a deletion of LRR3-6. Lgr6 is expressed on hair follicle stem cells that give rise to sebaceous glands, interfollicular epidermis, and hair follicles (4-6). It is also expressed on alveolar stem cells that differentiate into lung epithelium (7). It is often up-regulated in human gastric cancers (8). Lgr6 binds to R‑Spondins 1-4 and can enhance Wnt/beta-Catenin signaling through phosphorylation of the Wnt co-receptor LRP6 (9, 10).
  • References:
    1. de Lau, W. et al. (2014) Genes Dev. 28:305.
    2. Leushacke, M. and N. Barker (2012) Oncogene 31:3009.
    3. Hsu, S.Y. et al. (2000) Mol. Endocrinol. 14:1257.
    4. Snippert, H.J. et al. (2010) Science 327:1385.
    5. Liao, X.-H. and H. Nguyen (2014) Exp. Dermatol. 23:195.
    6. Nath, M. et al. (2011) Cell Tissue Res. 344:435.
    7. Oeztuerk-Winder, F. et al. (2012) EMBO J. 31:3431.
    8. Steffen, J.S. et al. (2012) Virchows Arch. 461:355.
    9. Gong, X. et al. (2012) PLoS ONE 7:e37137.
    10. de Lau, W. et al. (2011) Nature 476:293.
  • Long Name:
    Leucine-rich Repeat Containing G Protein-coupled Receptor 6
  • Entrez Gene IDs:
    59352 (Human); 329252 (Mouse); 498233 (Rat)
  • Alternate Names:
    FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631
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