Recombinant Human LILRA4/CD85g/ILT7 Protein, CF

    
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  • Purity
    >90%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA4/CD85g/ILT7 is coated at 1 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 of 20-120 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived human LILRA4/CD85g/ILT7 protein
    Glu24-Asn446, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Glu24
  • Predicted Molecular Mass
    47 kDa
  • SDS-PAGE
    60-73 kDa, reducing conditions
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8914-T4
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
When Recombinant Human LILRA4/CD85g/ILT7 is coated at 1μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of
20-120 ng/mL.
Background: LILRA4/CD85g/ILT7
LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Alternative splicing generates an additional isoform that lacks the signal peptide and a portion of the first Ig-like domain. LILRA4 is expressed on plasmacytoid dendritic cells (pDC) but is down-regulated in response to TLR9 signaling (3-5). Antibody mediated crosslinking of LILRA4 on pDC inhibits the production of type I interferons following TLR7 or TLR9 stimulation (3, 4, 6). It also blocks the up-regulation of CCR7 but enhances the up-regulation of Integrin  beta 7 on TLR7/9-stimulated pDC (6). LILRA4 associates with the ITAM-containing adaptor protein Fc epsilon RI gamma (3, 4, 6), and this complex binds to cell surface BST2/Tetherin which is expressed on monocytes, plasmacytoid and myeloid dendritic cells, B cells, and activated CD4+ and CD8+ T cells (5, 8). This interaction inhibits the TLR-induced pDC production of type I interferons, IL-6, and TNF-alpha (8).
  • References:
    1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
    2. Young, N.T. et al. (2001) Immunogenetics 53:270.
    3. Cho, M. et al. (2008) Int. Immunol. 20:155.
    4. Cao, W. et al. (2006) J. Exp. Med. 203:1399.
    5. Tavano, B. et al. (2013) J. Immunol. 190:2622.
    6. Tsukamoto, N. et al. (2009) Clin. Cancer Res. 15:5733.
    7. Tavano, B. and A. Boasso (2014) PLoS ONE 9:e89414.
    8. Cao, W. et al. (2009) J. Exp. Med. 206:1603.
  • Long Name:
    Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4
  • Entrez Gene IDs:
    23547 (Human)
  • Alternate Names:
    CD85 antigen-like family member G; CD85g antigen; CD85g; ILT7; ILT-7; ILT7MGC129598; Immunoglobulin-like transcript 7; leukocyte immunoglobulin-like receptor subfamily A member 4; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member4; LILRA4; MGC129597
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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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