Recombinant Human LILRA4/CD85g/ILT7 Protein, CF

Catalog # Availability Size / Price Qty
8914-T4-050
Recombinant Human LILRA4/CD85g/ILT7 Protein, CF Binding Activity
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Recombinant Human LILRA4/CD85g/ILT7 Protein, CF Summary

Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA4/CD85g/ILT7 is coated at 1 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 of 20-120 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human LILRA4/CD85g/ILT7 protein
Glu24-Asn446, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu24
Predicted Molecular Mass
47 kDa
SDS-PAGE
60-73 kDa, reducing conditions

Product Datasheets

8914-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human LILRA4/CD85g/ILT7 Protein, CF Binding Activity View Larger

When Recombinant Human LILRA4/CD85g/ILT7 is coated at 1μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of
20-120 ng/mL.

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Background: LILRA4/CD85g/ILT7

LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Alternative splicing generates an additional isoform that lacks the signal peptide and a portion of the first Ig-like domain. LILRA4 is expressed on plasmacytoid dendritic cells (pDC) but is down-regulated in response to TLR9 signaling (3-5). Antibody mediated crosslinking of LILRA4 on pDC inhibits the production of type I interferons following TLR7 or TLR9 stimulation (3, 4, 6). It also blocks the up-regulation of CCR7 but enhances the up-regulation of Integrin  beta 7 on TLR7/9-stimulated pDC (6). LILRA4 associates with the ITAM-containing adaptor protein Fc epsilon RI gamma (3, 4, 6), and this complex binds to cell surface BST2/Tetherin which is expressed on monocytes, plasmacytoid and myeloid dendritic cells, B cells, and activated CD4+ and CD8+ T cells (5, 8). This interaction inhibits the TLR-induced pDC production of type I interferons, IL-6, and TNF-alpha (8).

References
  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Young, N.T. et al. (2001) Immunogenetics 53:270.
  3. Cho, M. et al. (2008) Int. Immunol. 20:155.
  4. Cao, W. et al. (2006) J. Exp. Med. 203:1399.
  5. Tavano, B. et al. (2013) J. Immunol. 190:2622.
  6. Tsukamoto, N. et al. (2009) Clin. Cancer Res. 15:5733.
  7. Tavano, B. and A. Boasso (2014) PLoS ONE 9:e89414.
  8. Cao, W. et al. (2009) J. Exp. Med. 206:1603.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4
Entrez Gene IDs
23547 (Human)
Alternate Names
CD85 antigen-like family member G; CD85g antigen; CD85g; ILT7; ILT-7; ILT7MGC129598; Immunoglobulin-like transcript 7; leukocyte immunoglobulin-like receptor subfamily A member 4; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member4; LILRA4; MGC129597

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