Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

R&D Systems | Catalog # 8989-T2

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein (8989-T2)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q8NHL6

Structure / Form

Monomer

Applications

Bioactivity
View all LILRB1/CD85j/ILT2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRB1/CD85j/ILT2 protein
Human LILRB1
(Gly24-His458)
Accession # Q8NHL6		 HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

48 kDa

SDS-PAGE

65-82 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRB1/CD85j/ILT2 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 of 0.02-0.2 μg/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 8989-T2 in the following applications:

Scientific Data Images for Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

When Recombinant Human LILRB1/CD85j/ILT2 His-tag (Catalog # 8989-T2) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (914-AN) binds with a typical ED50 of 0.02-0.2 μg/mL.

Formulation, Preparation, and Storage

8989-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LILRB1/CD85j/ILT2

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma delta T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HLA-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems
in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma delta T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
  3. Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
  4. Harly, C. et al. (2011) Blood 117:2864.
  5. Cosman, D. et al. (1997) Immunity 7:273.
  6. Young, N.T. et al. (2001) J. Immunol. 166:3933.
  7. Young, N.T. et al. (2008) Blood 111:3090.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
  10. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  11. Ince, M.N. et al. (2004) Immunology 112:531.
  12. Saverino, D. et al. (2000) J. Immunol. 165:3742.
  13. Saverino, D. et al. (2002) J. Immunol. 168:207.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1

Alternate Names

CD85j, ILT2, LIR1, MIR7

Entrez Gene IDs

10859 (Human)

Gene Symbol

LILRB1

UniProt

Q8NHL6

Additional LILRB1/CD85j/ILT2 Products

Product Documents for Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

For research use only

Citations for Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

Customer Reviews for Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/01/2021
    immobilize HLA-G and flow ILT2-his
    Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF 8989-T2
  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/06/2020
    Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)
    Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF 8989-T2

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes