Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10429-T4-050
Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein, CF Data
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Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Apolipoprotein E3/ApoE3 (Catalog # 4144-AE) is immobilized at 5 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera (Catalog # 10429-T4) that produces 50% of the optimal binding response is approximately 0.8-5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human LILRB4/CD85k/ILT3 protein
Human LILRB4/CD85k/ILT3
(Gly24-Glu259)
Accession # NP_001265355.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53 kDa
SDS-PAGE
55-75 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10429-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

When Recombinant Human Apolipoprotein E3 (Catalog # 4144-AE) is immobilized at 5 µg/mL, 100 µL/well, the concentration of Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera (Catalog #10429-T4) that produces 50% of the optimal binding response is approximately 0.8-5 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein (Catalog # 10429-T4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB4/CD85k/ILT3

ILT3, also known as LILRB4, CD85k, and LIR5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature human ILT3 consists of an extracellular domain (ECD) with two Ig-like domains, a transmembrane segment, and a cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). The mature ECD of human ILT3 shares 44% amino acid identity with mouse ILT3. Alternative splicing of human ILT3 generates an isoform that lacks the first ITIM and a secreted isoform that circulates in the serum of cancer patients (3, 4). ILT3 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (2, 5, 6). Ligation of ILT3 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (2, 4, 7, 8). Soluble ILT3 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (4, 7, 9). In turn, CD8+ Ts cells induce ILT3 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (5, 6, 8, 10, 11). Recently, a novel anti-LILRB4 CAR-T Cell was been used to treat monocytic acute myeloid leukemia in humanized hematopoietic-reconstituted mice models (12).

References
  1. Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
  2. Cella, M. et al. (1997) J. Exp. Med. 185:1743.
  3. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
  4. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
  5. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
  6. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
  7. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
  8. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  9. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
  10. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
  11. Brenk, M. et al. (2009) J. Immunol. 183:145.
  12. John, S. et al. (2018) Mol. Ther. 26:2487.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs
11006 (Human); 14728 (Mouse)
Alternate Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4

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