Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF

Formulations:
Catalog # Availability Size / Price Qty
355-BEC-010/CF
355-BEC-025/CF
355-BEC-100/CF
Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF Data
2 Images
Product Details
Citations (6)
FAQs
Reviews

Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.08-0.48 µg/mL.
Source
E. coli-derived BMP-2 protein
Ala284-Arg396
This form is not detected using human BMP-2 Quantikine ELISA (Catalog # DBP200).
Accession #
N-terminal Sequence
Analysis
Ala284
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.8 kDa (monomer)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

355-BEC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

355-BEC

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BEC/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50for this effect is 0.08-0.48 μg/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human/Mouse/Rat BMP-2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BMP-2

Human BMP-2 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse and rat BMP-2 are 100% identical. BMP-2 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-2 is a disulfide-linked homodimer of the carboxy-terminal 114 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. One BMP type II and two BMP type I receptors have been identified. In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both BMP receptor type Is can bind BMP-2 with high-affinity in the absence of BMP receptor type II. 

References
  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49
  2. Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
  3. Reddi, A.H. (1998) Nature Biotechnology 16: 247.
Long Name
Bone Morphogenetic Protein 2
Entrez Gene IDs
650 (Human); 12156 (Mouse); 29373 (Rat)
Alternate Names
BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC

Citations for Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Vitamin D-regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification
    Authors: L Nguyen-Yam, KI Tanaka, R St-Arnaud, D Goltzman
    JCI Insight, 2019;4(13):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6
    Nat Commun, 2016;7(0):13247.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Quantitative Proteomics of the SMAD (Suppressor of Mothers against Decapentaplegic) Transcription Factor Family Identifies Importin 5 as a Bone Morphogenic Protein Receptor SMAD-specific Importin
    Authors: H Th Marc Timme
    J. Biol. Chem., 2016;291(46):24121-24132.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury.
    Authors: Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M
    Stem Cells Transl Med, 2014;3(3):334-45.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ,
    BMC Cell Biol., 2009;10(0):20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through hemojuvelin and BMP2/4.
    Authors: Lin L, Valore EV, Nemeth E, Goodnough JB, Gabayan V, Ganz T
    Blood, 2007;110(6):2182-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    • Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  2. What is the recommended method for reconstitution of a lyophilized protein or antibody?

    • Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.  

       

  3. Are R&D Systems recombinant proteins and antibodies sterile?

    • Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

  4. What is the difference between the two Recombinant Human/Mouse/Rat BMP-2 Proteins (Catalog # 355-BM and 355-BEC)?

    • Catalog # 355-BM is produced in a mammalian system using CHO cells and exhibits better activity in our routine QC bioassay, while Catalog # 355-BEC is produced in an E. coli expression system and is less expensive. Bioactivity of both Human/Mouse/Rat BMP-2 Proteins is measured in an alkaline phosphatase assay using by ATDC5 mouse chondrogenic cells. For 355-BM, the ED50 for this effect is 40-200 ng/mL, while for 355-BEC, the ED50 range is 80-480 ng/mL. We recommend reviewing our list of publications under the Citations tab for both 355-BM and 355-BEC to determine which of the two proteins is most suitable for your experiment.

  5. What is the sequence homology between human, mouse and rat BMP-2

    • Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2.

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF

There are currently no reviews for this product. Be the first to review Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF and earn rewards!

Have you used Recombinant Human/Mouse/Rat BMP-2 (E. coli-expressed), CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review