Recombinant Human Parkin Protein, CF Summary
Reaction conditions will need to be optimized for each specific application. As supplied, Parkin has negligible E3 ligase activity as determined by the lack of autoubiquitination in an in vitro assay. Parkin ligase activity is greatly enhanced by phosphorylation with PINK1 kinase (AP-180) or by the addition of low concentrations of phosphorylated Ubiquitin (U-102).
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 25 mM Tris pH 8.5, 200 mM NaCl, 0.03% Brij35, 10% (v/v) Glycerol, 5 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene) is an essential part of the cellular machinery that participates in the removal of damaged mitochondria. Mutations in PARK2 are known to cause a form of Parkinson's disease known as autosomal recessive juvenile Parkinson's disease (AR-JP), and the mechanisms by which defective Parkin ligase contributes to the dopaminergic cell death in this disease is an area of intense investigation.
Reported substrates for Parkin include BCL2, GPR37, MIRO1, MFN1, MFN2, TOMM20, USP30, and many others. Parkin (an RBR-class Ubiquitin ligase) structures have recently been reported by multiple groups, and reveal that the ligase is folded upon itself to produce an auto-inhibited state. The auto-inhibition is relieved by interactions with PINK1 kinase (which can phosphorylate both Parkin and Ubiquitin at serine residue number 65) and pS65 phospho-Ubiquitin by mechanisms that are under investigation.
In vitro, Parkin may be activated by treatment with recombinant PINK1, or addition of low concentrations of pS65-phosphoubiquitin. Parkin has been reported to generate poly-Ubiquitin chains in K6, K11, K48, and K63 linkages both in vitro and in vivo. This recombinant protein is untagged.
Bingol, B. et al. (2014) Nature 510: 370
Ordureau, A. et al. (2014) Mol. Cell 56: 360
Riley, B.E. et al. (2013) Nat. Comm. 4: doi:10.1038/ncomms2982
Saraff, S.A. et al. (2013) Nature 496: 372
Spratt, D.E. et al. (2013) Nat. Comm. 4: doi:10.1038/ncomms2983
Trempe, J.F. et al. (2013) Science 340: 1451
Wauer T. et. al. (2015) Nature 524: 370
Wauer T. & Komander, D. (2013) EMBO J 32: 2099
Citation for Recombinant Human Parkin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Parkin is an E3 ligase for the ubiquitin-like modifier FAT10, which inhibits Parkin activation and mitophagy
Authors: ND Roverato, C Sailer, N Catone, A Aichem, F Stengel, M Groettrup
Cell Reports, 2021;34(11):108857.
Sample Types: Whole Cells
At what molecular weight does Recombinant Human Parkin Protein (Catalog # E3-160) run in SDS-PAGE?
The theoretical molecular weight for this protein is 52 kDa, however many RING-containing proteins (such as Parkin) run at a lower apparent molecular weight than expected. We have found that this protein runs around 40 kDa.
Reviews for Recombinant Human Parkin Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Parkin Protein, CF and earn rewards!
Have you used Recombinant Human Parkin Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image