Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress. PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2. Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by PINK-mediated phosphorylation of PARK2 at serine 65, and PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65). This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.
Recombinant Human Phospho-Ubiquitin (S65) Protein, CF
R&D Systems | Catalog # U-102
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human Phospho-Ubiquitin (S65) Protein (U-102)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived human Ubiquitin protein
Met1 - Gly76
Phosphorylated on Ser 65.
Met1 - Gly76
Phosphorylated on Ser 65.
Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
8.6 kDa
Activity
Reaction conditions will need to be optimized for each specific application.
Reviewed Applications
Read 1 review rated 5 using U-102 in the following applications:
Formulation, Preparation, and Storage
U-102
| Formulation | Supplied as a solution in HEPES. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Ubiquitin
References
- Matsuda N. et al. (2010) J. Cell Biol. 189: 211.
- Kane L.A. et al. (2014) J. Cell Biol. 205:143.
- Ordureau A. et al. (2014) Mol Cell. 56: 360.
- Vives-Bauza C. et al. (2010) Proc. Natl. Acad. Sci. 107: 378.
- Wall C.E. et al. (2019) Cell Reports 29: 3280.
- Wauer T. et al. (2015) EMBO J. 34: 307.
Alternate Names
UBB
Gene Symbol
UBB
UniProt
Additional Ubiquitin Products
Product Documents for Recombinant Human Phospho-Ubiquitin (S65) Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Phospho-Ubiquitin (S65) Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Phospho-Ubiquitin (S65) Protein, CF
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