Parkin in Human Brain. Parkin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Goat Anti-Human Parkin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1438) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Parkin is a 465 amino acid protein with an N-terminal ubiquitin-like domain, a linker region, a C-terminal TRIAD domain consisting of two RING finger motifs flanking a cysteine-rich IBR (in between RING fingers) motif. Mutations in Parkin is a major cause of autosomal recessive juvenile parkinsonism. Parkin-2 lacks exon 5 which encodes amino acid residues 179-206 of Parkin.
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