Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
9049-B7-100
Graph showing bioactivity of Human PD-L1 protein (B7-H1 protein)
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (12)

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF Summary

Why choose R&D Systems PD-L1 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

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Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L1/B7-H1 is immobilized at 0.025 µg/mL (100 µL/well), the concentration of Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human PD-L1/B7-H1 protein
Phe19-Thr239, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Phe19
Predicted Molecular Mass
26 kDa
SDS-PAGE
31-41 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9049-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of PD-L1 Protein View Larger

When Recombinant Human PD-L1 / B7-H1 is immobilized at 0.025 µg/mL, it binds to Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) with an ED50 of 0.05-0.3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).

References
  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Dong, H. et al. (1999) Nat. Med. 5:1365.
  3. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
  4. Tamura, H. et al. (2001) Blood 97:1809.
  5. Chen, L. et al. (2007) J. Immunol. 178:6634.
  6. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  7. Tsushima, F. et al. (2007) Blood 110:180.
  8. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  9. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  10. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  11. Dong, H. et al. (2002) Nat. Med. 8:793.
  12. Azuma, T. et al. (2008) Blood 111:3635.
  13. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  14. Park, J.-J. et al. (2010) Blood 116:1291.
  15. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  16. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Citation for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Rapid PD-L1 detection in tumors with PET using a highly specific peptide
    Authors: Samit Chatterjee
    Biochem. Biophys. Res. Commun, 2016;0(0):.
    Applications: Bioassay

FAQs

  1. The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    • Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  2. What is the recommended method for reconstitution of a lyophilized protein or antibody?

    • Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.  

       

  3. Are R&D Systems recombinant proteins and antibodies sterile?

    • Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

  4. Is there a linker between the Fc and the His region of Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7)?

    • The sequence for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) is Phe19-Thr239 (Accession # Q9NZQ7) fused directly to a C-terminal 6-His tag. This protein does not contain a linker sequence.

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Reviews for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

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Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 10/24/2019
Application: In vitro bioactivity in cell culture

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 01/11/2019
Application: In vivo study

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 12/21/2018
Application: In vitro bioactivity in cell culture

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 10/10/2018
Application: Immunoassay Standard

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 08/26/2018
Application: Immunoassay Standard

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 04/12/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 03/30/2018
Application: SDS-PAGE Control

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 03/15/2018
Application: SDS-PAGE Control

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 03/11/2018
Application: SDS-PAGE Control

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 12/18/2017
Application: SDS-PAGE Control

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 11/27/2017
Application: Binding assay/Protein-protein interaction

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 11/18/2017
Application: Binding assay/Protein-protein interaction