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Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

R&D Systems | Catalog # 9049-B7

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human PD-L1/B7-H1 His-tag Protein (9049-B7)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q9NZQ7

Applications

Binding Activity
View all PD-L1/B7-H1 Proteins and Enzymes »

Why choose R&D Systems PD-L1 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Find the Fc chimera PD-L1 protein here PD-L1 Fc

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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PD-L1/B7-H1 protein
Phe19-Thr239, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

26 kDa

SDS-PAGE

31-41 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PD-L1/B7-H1 is immobilized at 0.025 µg/mL (100 µL/well), the concentration of Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

Reviewed Applications

Read 12 reviews rated 5 using 9049-B7 in the following applications:

Scientific Data Images for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Graph showing bioactivity of Human PD-L1 protein (B7-H1 protein)

Bioactivity of PD-L1 Protein

When Recombinant Human PD-L1 / B7-H1 is immobilized at 0.025 µg/mL, it binds to Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) with an ED50 of 0.05-0.3 µg/mL.

Formulation, Preparation, and Storage

9049-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Dong, H. et al. (1999) Nat. Med. 5:1365.
  3. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
  4. Tamura, H. et al. (2001) Blood 97:1809.
  5. Chen, L. et al. (2007) J. Immunol. 178:6634.
  6. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  7. Tsushima, F. et al. (2007) Blood 110:180.
  8. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  9. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  10. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  11. Dong, H. et al. (2002) Nat. Med. 8:793.
  12. Azuma, T. et al. (2008) Blood 111:3635.
  13. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  14. Park, J.-J. et al. (2010) Blood 116:1291.
  15. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  16. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9NZQ7

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

For research use only

Citations for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Customer Reviews for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (12)

5 out of 5
12 Customer Ratings
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  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/24/2019
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Jenna Nguyen
    Application: In vivo study
    Verified Customer | Posted 01/11/2019
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/21/2018
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 10/10/2018
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 08/26/2018
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 04/12/2018
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/30/2018
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/15/2018
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/11/2018
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Amit Kumar
    Application: SDS-PAGE Control
    Verified Customer | Posted 12/18/2017
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/27/2017
    Recombinant Human PD-L1/B7-H1 His-tag Protein, CF 9049-B7
  • Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/18/2017

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FAQs for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

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  • Q: Is there a linker between the PD-L1/B7-H1 protein and the His tag of Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7)?

    A: The sequence for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) is Phe19-Thr239 (Accession # Q9NZQ7) fused directly to a C-terminal 6-His tag. This protein does not contain a linker sequence.

  • Q: What are the differences between Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) and Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7)?

    A: Recombinant Human PD-L1/B7-H1 Protein, CF (Catalog # 9049-B7) consists of (Phe19-Thr239) from Accession # Q9NZQ7. It has a His tag but no Fc chimera. Activity for 9049-B7 is measured in a binding assay with Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD). Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog 156-B7) consists of the same region (Phe19-Thr239), but instead of a His tag, it has a linker region and an Fc chimera. Activity for 156-B7 is assessed in a cell-based assay measuring IL-2 production by human T lymphocytes.

  • Q: Is there a linker between the PD-L1/B7-H1 protein and the His tag of Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7)?

    A: The sequence for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) is Phe19-Thr239 (Accession # Q9NZQ7) fused directly to a C-terminal 6-His tag. This protein does not contain a linker sequence.

  • Q: What are the differences between Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) and Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7)?

    A: Recombinant Human PD-L1/B7-H1 Protein, CF (Catalog # 9049-B7) consists of (Phe19-Thr239) from Accession # Q9NZQ7. It has a His tag but no Fc chimera. Activity for 9049-B7 is measured in a binding assay with Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD). Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog 156-B7) consists of the same region (Phe19-Thr239), but instead of a His tag, it has a linker region and an Fc chimera. Activity for 156-B7 is assessed in a cell-based assay measuring IL-2 production by human T lymphocytes.

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