Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

  (1 citations)
(7 Reviews)
    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L1/B7-H1 is immobilized at 0.025 µg/mL (100 µL/well), the concentration of Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived human PD-L1/B7-H1 protein
    Phe19-Thr239, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Phe19
  • Predicted Molecular Mass
    26 kDa
  • SDS-PAGE
    31-41 kDa, reducing conditions
Product Datasheets

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9049-B7
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 500 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
WhenRecombinant Human PD-L1/B7-H1 is immobilized at 0.025 µg/mL,it binds to Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD) with an ED50 of 0.05-0.3 μg/mL.
Background: PD-L1/B7-H1
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).
  • References:
    1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
    2. Dong, H. et al. (1999) Nat. Med. 5:1365.
    3. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
    4. Tamura, H. et al. (2001) Blood 97:1809.
    5. Chen, L. et al. (2007) J. Immunol. 178:6634.
    6. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
    7. Tsushima, F. et al. (2007) Blood 110:180.
    8. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
    9. Cao, Y. et al. (2010) Cancer Res. 71:1235.
    10. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
    11. Dong, H. et al. (2002) Nat. Med. 8:793.
    12. Azuma, T. et al. (2008) Blood 111:3635.
    13. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
    14. Park, J.-J. et al. (2010) Blood 116:1291.
    15. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
    16. Herold, M. et al. (2015) J. Immunol. 195:3584.
  • Long Name:
    B7 Homolog 1
  • Entrez Gene IDs:
    29126 (Human); 60533 (Mouse); 499342 (Rat); 484186 (Canine); 102145573 (Cynomolgus Monkey)
  • Alternate Names:
    B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Rapid PD-L1 detection in tumors with PET using a highly specific peptide
    Authors: Samit Chatterjee
    Biochem. Biophys. Res. Commun, 2016;0(0):.
    Application: Bioassay

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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Average Rating: 5 (Based on 7 reviews)

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Binding assay/Protein-protein interaction Recombinant Human PD-L1/B7-H1 His-tag Protein, CF 9049-B7 Array 9049-B7
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Binding assay/Protein-protein interaction Recombinant Human PD-L1/B7-H1 His-tag Protein, CF 9049-B7
Binding assay/Protein-protein interaction: Recombinant Human PD-L1/B7-H1 His-tag Protein, CF [9049-B7].

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