Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7-100: R&D Systems

Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

(7 citations)
(8 Reviews)
  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 2‑10 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human PD-L1
    (Phe19-Thr239)
    Accession # Q9NZQ7
    DIEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Phe19
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    52 kDa (monomer)
  • SDS-PAGE
    70-75 kDa, reducing conditions
156-B7
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human PD-L1/B7-H1 Fc Chimera (Catalog # 156-B7) inhibits anti-CD3antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 2-10 μg/mL.
Background: PD-L1/B7-H1
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on
inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).
  • References:
    1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
    2. Dong, H. et al. (1999) Nat. Med. 5:1365.
    3. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
    4. Tamura, H. et al. (2001) Blood 97:1809.
    5. Chen, L. et al. (2007) J. Immunol. 178:6634.
    6. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
    7. Tsushima, F. et al. (2007) Blood 110:180.
    8. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
    9. Cao, Y. et al. (2010) Cancer Res. 71:1235.
    10. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
    11. Dong, H. et al. (2002) Nat. Med. 8:793.
    12. Azuma, T. et al. (2008) Blood 111:3635.
    13. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
    14. Park, J.-J. et al. (2010) Blood 116:1291.
    15. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
    16. Herold, M. et al. (2015) J. Immunol. 195:3584.
  • Long Name:
    B7 Homolog 1
  • Entrez Gene IDs:
    29126 (Human); 60533 (Mouse); 499342 (Rat); 484186 (Canine); 102145573 (Cynomolgus Monkey)
  • Alternate Names:
    B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Characterization of the anti-PD-1 antibody REGN2810 and its antitumor activity in human PD-1 knock-in mice
    Authors: E Burova, A Hermann, J Waite, T Potocky, V Lai, S Hong, M Liu, O Allbritton, A Woodruff, Q Wu, A D'Orvillie, E Garnova, A Rafique, W Poueymirou, J Martin, T Huang, D Skokos, J Kantrowitz, J Popke, M Mohrs, D MacDonald, E Ioffe, W Olson, I Lowy, A Murphy, G Thurston
    Mol. Cancer Ther, 2017;0(0):.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  2. B7-H1 antibodies lose antitumor activity due to activation of p38 MAPK that leads to apoptosis of tumor-reactive CD8(+) T cells
    Sci Rep, 2016;6(0):36722.
    Species: Human
    Sample Type: Protein
    Application: ELISA Standard
  3. PD-1/SHP-2 inhibits Tc1/Th1 phenotypic responses and the activation of T cells in the tumor microenvironment.
    Authors: Li J, Jie H, Lei Y, Gildener-Leapman N, Trivedi S, Green T, Kane L, Ferris R
    Cancer Res, 2015;75(3):508-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. IDO-independent suppression of T cell effector function by IFN-gamma-licensed human mesenchymal stromal cells.
    Authors: Chinnadurai R, Copland I, Patel S, Galipeau J
    J Immunol, 2014;192(4):1491-501.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
    Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
    PLoS ONE, 2014;9(12):e113396.
    Species: Human
    Sample Type: Serum
    Application: ELISA Standard
  6. MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers.
    Authors: Steidl C, Shah SP, Woolcock BW
    Nature, 2011;471(7338):377-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
    Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
    J. Immunol., 2006;177(12):8844-50.
Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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Average Rating: 4.8 (Based on 8 reviews)

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We have 7 reviews tested in 3 applications: Binding assay/Protein-protein interaction, Cell Adhesion, SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody .
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  Average
 Binding assay/Protein-protein interaction Anonymous 12/06/2017
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ApplicationBinding assay/Protein-protein interaction
  Excellent
 Binding assay/Protein-protein interaction Anonymous 12/01/2017
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ApplicationBinding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
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 Binding assay/Protein-protein interaction Anonymous 11/27/2017
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Binding assay/Protein-protein interaction Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
Binding assay/Protein-protein interaction: Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF [156-B7]

Summary

ApplicationBinding assay/Protein-protein interaction
  Excellent
 Binding assay/Protein-protein interaction Anonymous 11/18/2017
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ApplicationBinding assay/Protein-protein interaction
  Excellent
 Binding assay/Protein-protein interaction Anonymous 07/20/2017
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ApplicationBinding assay/Protein-protein interaction
  Excellent
 Cell Adhesion Anonymous 07/20/2017
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ApplicationCell Adhesion
SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody  Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
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Excellent
 SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody Anonymous 07/14/2017
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SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody  Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody : Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF [156-B7]

Summary

ApplicationSPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody

Other Experimental Details

Other Experimental DetailsSPR experiment
 Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7-100
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  Anonymous 05/23/2017
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 Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7-100
: Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF [156-B7-100]

Other Experimental Details

Other Experimental DetailsUsing Octet, we tested the ability of B7-H1/PD-L1 to bind recombinant PD-1 (R&D 8986-PD-100) and found them to specifically bind to each other.

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