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Recombinant Human PD-1 Fc Chimera Protein, CF

R&D Systems | Catalog # 1086-PD

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human PD-1 Fc Chimera Protein (1086-PD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q15116.3

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
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Why choose R&D Systems PD-1 Protein, Fc Chimera?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PD-1 protein
Human PD-1
(Leu25-Gln167)
Accession # Q15116.3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

42.6 kDa (monomer)

SDS-PAGE

60 - 70 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PD-1 Fc Chimera is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) binds with a typical ED50 of 0.15-0.75 μg/mL.

Reviewed Applications

Read 10 reviews rated 4.7 using 1086-PD in the following applications:

Scientific Data Images for Recombinant Human PD-1 Fc Chimera Protein, CF

Recombinant Human PD‑1 Fc Chimera Protein SEC-MALS.

Recombinant human PD-1/Fc (Catalog # 1086-PD) has a molecular weight (MW) of 125.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Graph showing bioactivity of Human PD-1 protein

Bioactivity of Human PD-1 Protein

When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 0.1 µg/mL, Recombinant Human B7-H1/PD-L1 Fc Chimera (156-B7) binds with a typical ED50 of 0.15-0.75 µg/mL.

Formulation, Preparation, and Storage

1086-PD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-1

PD-1 (Programmed Death-1 receptor), also known as CD279, is a receptor expressed on T cells responsible for modulating T cell activation. Like CTLA‑4, PD-1 is classified as an immune checkpoint inhibitory receptor. When PD-1 protein binds to PD-L1, it initiates a negative signaling cascade inhibiting activation of T cells. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. Normally, PD-1 helps keep T cells from attacking other cells in the body. However, many cancers take advantage of this by expressing high amounts of PD-L1 allowing cancer cells to evade the body's own immune response. Blocking the PD-1:PD-L1 interaction has proven successful in treating many different cancer types.

PD-1 protein is type I transmembrane receptor belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA‑4, ICOS, and BTLA (2-5). Mature human PD-1 consists of an extracellular region (ECD) with one immunoglobulin-like V‑type domain, a transmembrane domain, and a cytoplasmic region. The mature ECD of human PD-1 shares 61% amino acid sequence identity with mouse PD-1 ECD. PD-1 protein acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11).

References

  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  5. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
  6. Zhang, X. et al. (2004) Immunity 20:337.
  7. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
  8. Nishimura, H. et al. (1996) Int. Immunol. 8:773.
  9. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
  10. Butte, M.J. et al. (2007) Immunity 27:111.
  11. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
  12. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99:12293.
  13. Nogrady, B. (2014) Nature 513:S10.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Entrez Gene IDs

5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)

Gene Symbol

PDCD1

UniProt

Q15116.3

Additional PD-1 Products

Product Documents for Recombinant Human PD-1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human PD-1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human PD-1 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human PD-1 Fc Chimera Protein, CF (10)

4.7 out of 5
10 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 07/13/2024
    Recombinant Human PD-1 Fc Chimera Protein, CF 1086-PD
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/26/2020
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/15/2020
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/12/2020
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/22/2019
    Cells expressing a negative control (left) or hPD-L1 mCherry (right) were treated with 0.25 ug of hPD-1 hIgG1. Cells were analyzed by flow cytometry with binding detected using an anti-human 488 secondary antibody.
    Recombinant Human PD-1 Fc Chimera Protein, CF 1086-PD
  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/20/2018
  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/08/2017
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/03/2017
  • Name: Sam Mullapudi
    Verified Customer | Posted 04/21/2017
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Songbo Qiu
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/08/2016
    Sorry that I just rate the catalog based on my alike recombinant PD1 peptide. I have one question for the peptide. Owing the Ig like peptide with Fc fragment. So when we detect it binding to PDL1 on cell assay, it is difficult to get definite conclusion if it is PDL1 binding or others. My protocol is as below: -Cells with PDL1 high level was treated by PD1 like peptide, -4degree 1hr -collected and lysis -WB by anti-IgG to Fc recognization. Result: weak signal. you don't know if it is non-specific binding
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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