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Recombinant Human PD-1 Fc Chimera Protein, CF

  (10 citations)
(4 Reviews)     
Datasheet / CoA / SDS
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Citations (10)
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Reviews
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 Fc Chimera is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) binds with a typical ED50 of 0.15-0.75 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived human PD-1 protein
    Human PD-1
    (Leu25-Gln167)
    Accession # Q8IX89    		 IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
    Q8IX89
  • N-terminal Sequence
    Analysis
    Leu25
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    42.6 kDa (monomer)
  • SDS-PAGE
    60-70 kDa, reducing conditions
Product Datasheets
Product Datasheet

COA

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SDS
1086-PD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
  
Data Image
Bioactivity

Bioactivity PD-1 [Unconjugated]
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When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD)is coated at 0.1 μg/mL, Recombinant Human B7-H1/PD‑L1 Fc Chimera (Catalog # 156-B7) binds with a typical ED50 of 0.15-0.75 μg/mL.
Background: PD-1
Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces
co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).
  • References:
    1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
    2. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
    3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
    4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
    5. Watanabe, N et al. (2003) Nat. Immunol. 4:670.
    6. Zhang, X. et al. (2004) Immunity 20:337.
    7. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
    8. Nishimura, H et al. (1996) Int. Immunol. 8:773.
    9. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
    10. Butte, M.J. et al. (2007) Immunity 27:111.
    11. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
    12. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
    13. Nogrady, B. (2014) Nature 513:S10.
  • Long Name:
    Programmed Death-1
  • Entrez Gene IDs:
    5133 (Human); 18566 (Mouse); 301626 (Rat); 486213 (Canine); 102123659 (Cynomolgus Monkey)
  • Alternate Names:
    CD279 antigen; CD279; hPD-1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
 Applications
 Sample Type
  1. Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity
    Nat Commun, 2016;7(0):12632.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Aberrant PD-L1 expression through 3'-UTR disruption in multiple cancers
    Nature, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Rapid PD-L1 detection in tumors with PET using a highly specific peptide
    Authors: Samit Chatterjee
    Biochem. Biophys. Res. Commun, 2016;0(0):.
    Application: Bioassay
  4. B7-H1 enhances proliferation ability of gastric cancer stem-like cells as a receptor.
    Authors: Yang Y, Wu K, Zhao E, Li W, Shi L, Xie G, Jiang B, Wang Y, Li R, Zhang P, Shuai X, Wang G, Tao K
    Oncol Lett, 2015;9(4):1833-1838.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
    Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
    PLoS ONE, 2014;9(12):e113396.
    Species: Human
    Sample Type: Serum
    Application: ELISA Standard
  6. MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers.
    Authors: Steidl C, Shah SP, Woolcock BW
    Nature, 2011;471(7338):377-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Cross-linking of B7-H1 on EBV-transformed B cells induces apoptosis through reactive oxygen species production, JNK signaling activation, and fasL expression.
    Authors: Kim YS, Park GB, Lee HK, Song H, Choi IH, Lee WJ, Hur DY
    J. Immunol., 2008;181(9):6158-69.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
    Authors: Sakthivel P, Ramanujam R, Wang XB, Pirskanen R, Lefvert AK
    J. Neuroimmunol., 2007;193(1):149-55.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  9. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
    Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
    J. Immunol., 2006;177(12):8844-50.
  10. Phase I study of single-agent anti-programmed death-1 (MDX-1106) in refractory solid tumors: safety, clinical activity, pharmacodynamics, and immunologic correlates.
    Authors: Brahmer J, Drake C, Wollner I, Powderly J, Picus J, Sharfman W, Stankevich E, Pons A, Salay T, McMiller T, Gilson M, Wang C, Selby M, Taube J, Anders R, Chen L, Korman A, Pardoll D, Lowy I, Topalian S
    J Clin Oncol, 0;28(19):3167-75.
    Species: Human
    Sample Type: Serum
    Application: ELISA capture

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Primary Antibodies
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Human IgG1 Antibody

 WB , ELISA MAB9894  
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Recombinant Proteins
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Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

 BA 156-B7 9  
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Recombinant Human IgG1 Fc Protein, CF

 BA 110-HG 70
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

 RB01
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Average Rating: 4.3 (Based on 4 reviews)

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  Excellent
  Binding assay/Protein-protein interaction Anonymous 12/08/2017
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Application Binding assay/Protein-protein interaction
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Other Experimental Details

Other Experimental Details Sorry that I just rate the catalog based on my alike recombinant PD1 peptide. I have one question for the peptide. Owing the Ig like peptide with Fc fragment. So when we detect it binding to PDL1 on cell assay, it is difficult to get definite conclusion if it is PDL1 binding or others.My protocol is as below:-Cells with PDL1 high level was treated by PD1 like peptide,-4degree 1hr-collected and lysis-WB by anti-IgG to Fc recognization.Result: weak signal. you don't know if it is non-specific binding R&D Systems response: Thank you so much for taking the time to share your experience using R&D Systems Proteins. We value your opinion and use our online reviews as testimonials from our customers of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when our customers are unhappy. We have reached out to offer additional support with this product and are unable to reach you. Please contact a technical service representative at techsupport@bio-techne.com and allow us to further troubleshoot some of the concerns you have mentioned in your online review. We look forward to hearing from you.

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