Recombinant Human PD-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1086-PD-050
1086-PD-01M
Recombinant Human PD-1 Fc Chimera Protein, CF Bioactivity
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Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (8)

Recombinant Human PD-1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 Fc Chimera is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) binds with a typical ED50 of 0.15-0.75 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human PD-1 protein
Human PD-1
(Leu25-Gln167)
Accession # Q15116
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42.6 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1086-PD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity View Larger

When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 0.1 μg/mL, Recombinant Human B7-H1/PD‑L1 Fc Chimera (Catalog # 156-B7) binds with a typical ED50 of 0.15-0.75 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces
co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).

References
  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  5. Watanabe, N et al. (2003) Nat. Immunol. 4:670.
  6. Zhang, X. et al. (2004) Immunity 20:337.
  7. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
  8. Nishimura, H et al. (1996) Int. Immunol. 8:773.
  9. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
  10. Butte, M.J. et al. (2007) Immunity 27:111.
  11. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
  12. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
  13. Nogrady, B. (2014) Nature 513:S10.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

Citations for Recombinant Human PD-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Novel Human Anti-PD-L1 mAbs Inhibit Immune-Independent Tumor Cell Growth and PD-L1 Associated Intracellular Signalling
    Authors: M Passariell, AM D'Alise, A Esposito, C Vetrei, G Froechlich, E Scarselli, A Nicosia, C De Lorenzo
    Sci Rep, 2019;9(1):13125.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Upregulation of PD-L1 via HMGB1-activated IRF3 and NF-kB contributes to UV radiation-induced immune suppression
    Authors: W Wang, NM Chapman, B Zhang, M Li, M Fan, RN Laribee, MR Zaidi, LM Pfeffer, H Chi, ZH Wu
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. SA-49, a novel aloperine derivative, induces MITF-dependent lysosomal degradation of PD-L1
    Authors: N Zhang, Y Dou, L Liu, X Zhang, X Liu, Q Zeng, Y Liu, M Yin, X Liu, H Deng, D Song
    EBioMedicine, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1
    Authors: CW Li, SO Lim, EM Chung, YS Kim, AH Park, J Yao, JH Cha, W Xia, LC Chan, T Kim, SS Chang, HH Lee, CK Chou, YL Liu, HC Yeh, EP Perillo, AK Dunn, CW Kuo, KH Khoo, JL Hsu, Y Wu, JM Hsu, H Yamaguchi, TH Huang, AA Sahin, GN Hortobagyi, SS Yoo, MC Hung
    Cancer Cell, 2018;33(2):187-201.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity
    Authors: H Wang, H Yao, C Li, H Shi, J Lan, Z Li, Y Zhang, L Liang, JY Fang, J Xu
    Nat. Chem. Biol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Clinical implications of monitoring nivolumab immunokinetics in non-small cell lung cancer patients
    Authors: A Osa, T Uenami, S Koyama, K Fujimoto, D Okuzaki, T Takimoto, H Hirata, Y Yano, S Yokota, Y Kinehara, Y Naito, T Otsuka, M Kanazu, M Kuroyama, M Hamaguchi, T Koba, Y Futami, M Ishijima, Y Suga, Y Akazawa, H Machiyama, K Iwahori, H Takamatsu, I Nagatomo, Y Takeda, H Kida, EA Akbay, PS Hammerman, KK Wong, G Dranoff, M Mori, T Kijima, A Kumanogoh
    JCI Insight, 2018;3(19):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Capture)
  7. Deglycosylation of PD-L1 by 2-deoxyglucose reverses PARP inhibitor-induced immunosuppression in triple-negative breast cancer
    Authors: B Shao, CW Li, SO Lim, L Sun, YJ Lai, J Hou, C Liu, CW Chang, Y Qiu, JM Hsu, LC Chan, Z Zha, H Li, MC Hung
    Am J Cancer Res, 2018;8(9):1837-1846.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Aberrant PD-L1 expression through 3'-UTR disruption in multiple cancers
    Nature, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity
    Nat Commun, 2016;7(0):12632.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Rapid PD-L1 detection in tumors with PET using a highly specific peptide
    Authors: Samit Chatterjee
    Biochem. Biophys. Res. Commun, 2016;0(0):.
    Applications: Bioassay
  11. B7-H1 enhances proliferation ability of gastric cancer stem-like cells as a receptor.
    Authors: Yang Y, Wu K, Zhao E, Li W, Shi L, Xie G, Jiang B, Wang Y, Li R, Zhang P, Shuai X, Wang G, Tao K
    Oncol Lett, 2015;9(4):1833-1838.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
    Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
    PLoS ONE, 2014;9(12):e113396.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Standard)
  13. MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers.
    Authors: Steidl C, Shah SP, Woolcock BW
    Nature, 2011;471(7338):377-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Cross-linking of B7-H1 on EBV-transformed B cells induces apoptosis through reactive oxygen species production, JNK signaling activation, and fasL expression.
    Authors: Kim YS, Park GB, Lee HK, Song H, Choi IH, Lee WJ, Hur DY
    J. Immunol., 2008;181(9):6158-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
    Authors: Sakthivel P, Ramanujam R, Wang XB, Pirskanen R, Lefvert AK
    J. Neuroimmunol., 2007;193(1):149-55.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  16. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
    Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
    J. Immunol., 2006;177(12):8844-50.
  17. Phase I study of single-agent anti-programmed death-1 (MDX-1106) in refractory solid tumors: safety, clinical activity, pharmacodynamics, and immunologic correlates.
    Authors: Brahmer J, Drake C, Wollner I, Powderly J, Picus J, Sharfman W, Stankevich E, Pons A, Salay T, McMiller T, Gilson M, Wang C, Selby M, Taube J, Anders R, Chen L, Korman A, Pardoll D, Lowy I, Topalian S
    J Clin Oncol, 0;28(19):3167-75.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)

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Reviews for Recombinant Human PD-1 Fc Chimera Protein, CF

Average Rating: 4.6 (Based on 8 Reviews)

5 Star
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4 Star
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3 Star
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Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 01/15/2020
Application: Binding assay/Protein-protein interaction

Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 01/12/2020
Application: Binding assay/Protein-protein interaction

Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 11/22/2019
Application: Binding assay/Protein-protein interaction

Cells expressing a negative control (left) or hPD-L1 mCherry (right) were treated with 0.25 ug of hPD-1 hIgG1. Cells were analyzed by flow cytometry with binding detected using an anti-human 488 secondary antibody.


Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 08/20/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 12/08/2017
Application: Binding assay/Protein-protein interaction

Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 11/03/2017
Application: Binding assay/Protein-protein interaction

Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 04/21/2017

Recombinant Human PD-1 Fc Chimera Protein, CF
By Anonymous on 07/08/2016
Application: Binding assay/Protein-protein interaction

Sorry that I just rate the catalog based on my alike recombinant PD1 peptide. I have one question for the peptide. Owing the Ig like peptide with Fc fragment. So when we detect it binding to PDL1 on cell assay, it is difficult to get definite conclusion if it is PDL1 binding or others.
My protocol is as below:
-Cells with PDL1 high level was treated by PD1 like peptide,
-4degree 1hr
-collected and lysis
-WB by anti-IgG to Fc recognization.
Result: weak signal. you don't know if it is non-specific binding

R&D Systems response: Thank you so much for taking the time to share your experience using R&D Systems Proteins. We value your opinion and use our online reviews as testimonials from our customers of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when our customers are unhappy. We have reached out to offer additional support with this product and are unable to reach you. Please contact a technical service representative at techsupport@bio-techne.com and allow us to further troubleshoot some of the concerns you have mentioned in your online review. We look forward to hearing from you.