Recombinant Human PDGF-BB (CHO-expressed) Protein, CF

R&D Systems | Catalog # 11565-PB

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human PDGF-BB (CHO-expressed) Protein (11565-PB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P01127.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all PDGF-BB Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human PDGF-BB protein
Ser82-Thr190

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser 82

Predicted Molecular Mass

12 kDa

SDS-PAGE

11-13 kDa

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 2.50-25.0 ng/mL.

Scientific Data Images for Recombinant Human PDGF-BB (CHO-expressed) Protein, CF

Recombinant Human PDGF‑BB (CHO-expressed) Protein Bioactivity.

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 2.50‑25.0 ng/mL.

Recombinant Human PDGF‑BB (CHO-expressed) Protein SDS-PAGE.

2 μg/lane of Recombinant Human PDGF‑BB (CHO-expressed) Protein (Catalog # 11565-PB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 11- 13 kDa under reducing conditions.

Formulation, Preparation, and Storage

11565-PB
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in 4mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Entrez Gene IDs

5155 (Human); 24628 (Rat)

Gene Symbol

PDGFB

UniProt

P01127.1

Additional PDGF-BB Products

Product Documents for Recombinant Human PDGF-BB (CHO-expressed) Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human PDGF-BB (CHO-expressed) Protein, CF

For research use only

Related Research Areas

PDGF Family

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