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Recombinant Human PDGF-BB Protein, CF

R&D Systems | Catalog # 220-BB

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human PDGF-BB Protein (220-BB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P01127.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all PDGF-BB Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human PDGF-BB protein
Ser82-Thr190

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser82

Predicted Molecular Mass

12.3 kDa (monomer)

SDS-PAGE

12 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 1.5-6 ng/mL.

Reviewed Applications

Read 14 reviews rated 4.4 using 220-BB in the following applications:

Scientific Data Images for Recombinant Human PDGF-BB Protein, CF

Recombinant Human PDGF-BB Protein Bioactivity

Recombinant Human PDGF-BB Protein Bioactivity

Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.
Recombinant Human PDGF-BB Protein SDS-PAGE

Recombinant Human PDGF-BB Protein SDS-PAGE

1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.

Formulation, Preparation, and Storage

220-BB
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution

Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Entrez Gene IDs

5155 (Human); 24628 (Rat)

Gene Symbol

PDGFB

UniProt

P01127.1

Additional PDGF-BB Products

Product Documents for Recombinant Human PDGF-BB Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human PDGF-BB Protein, CF

For research use only

Related Research Areas

PDGF Family

Citations for Recombinant Human PDGF-BB Protein, CF

Customer Reviews for Recombinant Human PDGF-BB Protein, CF (14)

4.4 out of 5
14 Customer Ratings
5 Stars
57%
4 Stars
29%
3 Stars
14%
2 Stars
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1 Stars
0%

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  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 12/11/2025
    Recombinant Human PDGF-BB Protein, CF 220-BB
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 11/12/2024
    Recombinant Human PDGF-BB Protein, CF 220-BB
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 06/09/2021
    Recombinant Human PDGF-BB Protein, CF 220-BB
  • Recombinant Human PDGF-BB Protein, CF
    Name: Steven Moore
    Application: Immunoassay Standard
    Verified Customer | Posted 10/13/2020
    HMW high intact molecular weight LMW Super low molecular weight
    HMW high intact molecular weight LMW Super low molecular weight
    Recombinant Human PDGF-BB Protein, CF 220-BB
  • Recombinant Human PDGF-BB Protein, CF
    Name: Steven Moore
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 10/13/2020
    Proliferating cell nuclear antigen
    Recombinant Human PDGF-BB Protein, CF 220-BB
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/19/2019
  • Recombinant Human PDGF-BB Protein, CF
    Name: Justin Provencher
    Application: Cell Proliferation
    Verified Customer | Posted 10/09/2019
    Recombinant Human PDGF-BB Protein, CF 220-BB
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/20/2019
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/15/2018
  • Recombinant Human PDGF-BB Protein, CF
    Name: Tauseef Ahmad
    Application: Proof concept for delivery as therapeutic in a controlled delivery system
    Verified Customer | Posted 02/12/2018
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 02/07/2018
    Recombinant Human PDGF-BB Protein, CF 220-BB
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 09/11/2017
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 09/07/2017
  • Recombinant Human PDGF-BB Protein, CF
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 07/22/2016
    Recombinant Human PDGF-BB Protein, CF 220-BB

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FAQs for Recombinant Human PDGF-BB Protein, CF

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  • Q: Can Recombinant Human PDGF-BB Protein, CF (Catalog # 220-BB) be reconstituted in a buffer containing BSA?

    A: BSA preparations may contain minor impurities that can impact assay results, and assays involving Recombinant Human PDGF-BB Protein, CF (Catalog # 220-BB) can be influenced by these minor impurities. For this reason, we recommend reconstituting 220-BB to 100 ug/mL in 4 mM HCl. Including BSA in a buffer may be beneficial when diluting proteins to working concentrations less than 10 ug/mL. If BSA is included in an experiment involving 220-BB, we would recommend running a BSA-only control.

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