Recombinant Human PDGF-BB Protein, CF

  (95 citations)
(5 Reviews)
    
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Citations (95)
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  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
    The specific activity of Recombinant Human PDGF-BB is approximately 5.8 x 103 IU/μg, which is calibrated against recombinant human PDGF-BB WHO International Standard (NIBSC code: 94/728).
  • Source
    E. coli-derived human PDGF-BB protein
    Ser82-Thr190
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser82
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    12.3 kDa (monomer)
  • SDS-PAGE
    12 kDa, reducing conditions
Product Datasheets

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220-BB
 
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
 
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Images
Recombinant Human PDGF-BB (Catalog # 220-BB) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6 ng/mL.
1 μg/lane of Recombinant Human PDGF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.
Background: PDGF-BB
Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.
  • Long Name:
    Platelet-derived Growth Factor BB
  • Entrez Gene IDs:
    5155 (Human); 24628 (Rat)
  • Alternate Names:
    PDGFBB; PDGF-BB
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

95 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Diverse functions of clusterin promote and protect against the development of pulmonary fibrosis
    Authors: L Peix, IC Evans, DR Pearce, JK Simpson, TM Maher, RJ McAnulty
    Sci Rep, 2018;8(1):1906.
    Species: Human
    Sample Type: Whole Cells
  2. Methotrexate inhibits effects of platelet-derived growth factor and interleukin-1? on rheumatoid arthritis fibroblast-like synoviocytes
    Authors: B Bergström, H Carlsten, AH Ekwall
    Arthritis Res. Ther., 2018;20(1):49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. The chondroitin sulfate moiety mediates thrombomodulin-enhanced adhesion and migration of vascular smooth muscle cells
    Authors: VC Pai, IC Lo, YW Huang, IC Tsai, HP Cheng, GY Shi, HL Wu, MJ Jiang
    J. Biomed. Sci., 2018;25(1):14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation
    Authors: R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari
    BMC Cell Biol., 2017;18(1):21.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Src-homology protein tyrosine phosphatase-1 agonist, SC-43, reduces liver fibrosis
    Authors: TH Su, CW Shiau, P Jao, NJ Yang, WT Tai, CJ Liu, TC Tseng, HC Yang, CH Liu, KW Huang, TC Hu, YJ Huang, YM Wu, LJ Chen, PJ Chen, DS Chen, KF Chen, JH Kao
    Sci Rep, 2017;7(1):1728.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling
    Authors: D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó
    Kidney Blood Press. Res, 2017;42(1):16-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Urethral reconstruction with autologous urine-derived stem cells seeded in three-dimensional porous small intestinal submucosa in a rabbit model
    Authors: Y Liu, W Ma, B Liu, Y Wang, J Chu, G Xiong, L Shen, C Long, T Lin, D He, D Butnaru, L Alexey, Y Zhang, D Zhang, G Wei
    Stem Cell Res Ther, 2017;8(1):63.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
    Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
    PLoS Pathog., 2017;13(4):e1006281.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Combination therapy using molecular-targeted drugs modulates tumor microenvironment and impairs tumor growth in renal cell carcinoma
    Authors: H Kitano, Y Kitadai, J Teishima, R Yuge, S Shinmei, K Goto, S Inoue, T Hayashi, K Sentani, W Yasui, A Matsubara
    Cancer Med, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during H2O2 exposure
    Authors: M Dagnell, PE Pace, Q Cheng, J Frijhoff, A Östman, ESJ Arnér, MB Hampton, CC Winterbour
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. microRNA let-7g suppresses PDGF-induced conversion of vascular smooth muscle cell into the synthetic phenotype
    Authors: TM Wang, KC Chen, PY Hsu, HF Lin, YS Wang, CY Chen, YC Liao, SH Juo
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
    Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
    Sci Rep, 2017;7(1):16439.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  13. Folic acid inhibits dedifferentiation of PDGF-BB-induced vascular smooth muscle cells by suppressing mTOR/P70S6K signaling
    Authors: S Pan, H Lin, H Luo, F Gao, L Meng, C Zhou, C Jiang, Y Guo, Z Ji, J Chi, H Guo
    Am J Transl Res, 2017;9(3):1307-1316.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  14. Interferon-? blocks signalling through PDGFR? in human brain pericytes
    J Neuroinflammation, 2016;13(1):249.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Platelet-Derived Growth Factor in the Ovarian Follicle Attracts the Stromal Cells of the Fallopian Tube Fimbriae
    PLoS ONE, 2016;11(7):e0158266.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Mesenchymal stem cells from cortical bone demonstrate increased clonal incidence, potency, and developmental capacity compared to their bone marrow-derived counterparts
    J Tissue Eng, 2016;7(0):2041731416661.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  17. Integrative functional genomics identifies regulatory mechanisms at coronary artery disease loci
    Nat Commun, 2016;7(0):12092.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Mesenchymal Stem Cells Reshape and Provoke Proliferation of Articular Chondrocytes by Paracrine Secretion
    Sci Rep, 2016;6(0):32705.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Bioassay
  19. Pericyte-fibroblast transition promotes tumor growth and metastasis
    Proc Natl Acad Sci USA, 2016;113(38):E5618-E5627.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. miR-184 exhibits angiostatic properties via regulation of Akt and VEGF signaling pathways
    Authors: Robert M Lavker
    FASEB J., 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. c-Jun-N-terminal phosphorylation regulates DNMT1 expression and genome wide methylation in gliomas
    Authors: DH Heiland, R Ferrarese, R Claus, F Dai, AP Masilamani, E Kling, A Weyerbrock, T Kling, S Nelander, MS Carro
    Oncotarget, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Biological characteristics of embryonic epicardial cells in vitro correlate with embryonic day
    Authors: Xiaoming Wei
    Acta Biochim. Biophys. Sin. (Shanghai), 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Zedoarondiol Inhibits Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle Cells Proliferation via Regulating AMP-Activated Protein Kinase Signaling Pathway
    Authors: Huimin Mao
    Cell. Physiol. Biochem, 2016;40(6):1506-1520.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  24. Hyperplastic Growth of Pulmonary Artery Smooth Muscle Cells from Subjects with Pulmonary Arterial Hypertension Is Activated through JNK and p38 MAPK.
    Authors: Wilson J, Yu J, Taylor L, Polgar P
    , 2015;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Thrombomodulin promotes corneal epithelial wound healing.
    Authors: Huang, Yi-Hsun, I, Ching-Ch, Kuo, Cheng-Hs, Hsu, Yun-Yan, Lee, Fang-Tzu, Shi, Guey-Yue, Tseng, Sung-Hue, Wu, Hua-Lin
    PLoS ONE, 2015;10(3):e0122491.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Pyrroloquinoline-quinone suppresses liver fibrogenesis in mice.
    Authors: Jia D, Duan F, Peng P, Sun L, Ruan Y, Gu J
    PLoS ONE, 2015;10(3):e0121939.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  27. Fanconi Anemia Mesenchymal Stromal Cells-Derived Glycerophospholipids Skew Hematopoietic Stem Cell Differentiation Through Toll-Like Receptor Signaling.
    Authors: Amarachintha S, Sertorio M, Wilson A, Li X, Pang Q
    Stem Cells, 2015;33(11):3382-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.
    Authors: Bogershausen N, Tsai I, Pohl E, Kiper P, Beleggia F, Percin E, Keupp K, Matchan A, Milz E, Alanay Y, Kayserili H, Liu Y, Banka S, Kranz A, Zenker M, Wieczorek D, Elcioglu N, Prontera P, Lyonnet S, Meitinger T, Stewart A, Donnai D, Strom T, Boduroglu K, Yigit G, Li Y, Katsanis N, Wollnik B
    J Clin Invest, 2015;125(9):3585-99.
  29. Hsp90alpha and Hsp90beta together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing.
    Authors: Jayaprakash P, Dong H, Zou M, Bhatia A, O'Brien K, Chen M, Woodley D, Li W
    J Cell Sci, 2015;128(8):1475-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis.
    Authors: Hayes B, Riehle K, Shimizu-Albergine M, Bauer R, Hudkins K, Johansson F, Yeh M, Mahoney W, Yeung R, Campbell J
    PLoS ONE, 2014;9(3):e92925.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Pigment epithelium-derived factor 34-mer peptide prevents liver fibrosis and hepatic stellate cell activation through down-regulation of the PDGF receptor.
    Authors: Tsai T, Shih S, Ho T, Ma H, Liu M, Chen S, Tsao Y
    PLoS ONE, 2014;9(4):e95443.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  32. Imatinib mesylate exerts anti-proliferative effects on osteosarcoma cells and inhibits the tumour growth in immunocompetent murine models.
    Authors: Gobin B, Moriceau G, Ory B, Charrier C, Brion R, Blanchard F, Redini F, Heymann D
    PLoS ONE, 2014;9(3):e90795.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  33. Multiple receptor tyrosine kinases converge on microRNA-134 to control KRAS, STAT5B, and glioblastoma.
    Authors: Zhang, Y, Kim, J, Mueller, A C, Dey, B, Yang, Y, Lee, D-h, Hachmann, J, Finderle, S, Park, D M, Christensen, J, Schiff, D, Purow, B, Dutta, A, Abounader, R
    Cell Death Differ, 2014;21(5):720-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. Lutein inhibits the migration of retinal pigment epithelial cells via cytosolic and mitochondrial Akt pathways (lutein inhibits RPE cells migration).
    Authors: Su C, Chan C, Chen H, Wu C, Hsiao C, Lee P, Lin V, Hung C
    Int J Mol Sci, 2014;15(8):13755-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. Rho GTPases RhoA and Rac1 mediate effects of dietary folate on metastatic potential of A549 cancer cells through the control of cofilin phosphorylation.
    Authors: Oleinik N, Helke K, Kistner-Griffin E, Krupenko N, Krupenko S
    J Biol Chem, 2014;289(38):26383-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. Anti-fibrotic effects of nintedanib in lung fibroblasts derived from patients with idiopathic pulmonary fibrosis.
    Authors: Hostettler K, Zhong J, Papakonstantinou E, Karakiulakis G, Tamm M, Seidel P, Sun Q, Mandal J, Lardinois D, Lambers C, Roth M
    Respir Res, 2014;15(1):157.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. Human corneal stromal stem cells exhibit survival capacity following isolation from stored organ-culture corneas.
    Authors: Kureshi A, Funderburgh J, Daniels J
    Invest Ophthalmol Vis Sci, 2014;55(11):7583-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  38. Antifibrotic and anti-inflammatory activity of the tyrosine kinase inhibitor nintedanib in experimental models of lung fibrosis.
    Authors: Wollin L, Maillet I, Quesniaux V, Holweg A, Ryffel B
    J Pharmacol Exp Ther, 2014;349(2):209-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  39. Inhibition of receptor signaling and of glioblastoma-derived tumor growth by a novel PDGFRbeta aptamer.
    Authors: Camorani S, Esposito C, Rienzo A, Catuogno S, Iaboni M, Condorelli G, de Franciscis V, Cerchia L
    Mol Ther, 2014;22(4):828-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  40. TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
    Authors: Lo H, Lai T, Li C, Wu W
    Acta Pharmacol Sin, 2014;35(3):339-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  41. Coronary heart disease-associated variation in TCF21 disrupts a miR-224 binding site and miRNA-mediated regulation.
    Authors: Miller C, Haas U, Diaz R, Leeper N, Kundu R, Patlolla B, Assimes T, Kaiser F, Perisic L, Hedin U, Maegdefessel L, Schunkert H, Erdmann J, Quertermous T, Sczakiel G
    PLoS Genet, 2014;10(3):e1004263.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development.
    Authors: Kondo, Shunya, Bottos, Alessia, Allegood, Jeremy C, Masson, Regis, Maurer, Francisc, Genoud, Christel, Kaeser, Patrick, Huwiler, Andrea, Murakami, Masato, Spiegel, Sarah, Hynes, Nancy E
    PLoS ONE, 2014;9(4):e94114.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  43. Integration of proteomic and transcriptomic profiles identifies a novel PDGF-MYC network in human smooth muscle cells.
    Authors: Yang, Wei, Ramachandran, Aruna, You, Sungyong, Jeong, HyoBin, Morley, Samantha, Mulone, Michelle, Logvinenko, Tanya, Kim, Jayoung, Hwang, Daehee, Freeman, Michael, Adam, Rosalyn
    Cell Commun Signal, 2014;12(1):44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  44. Thrombin induces epithelial-mesenchymal transition and collagen production by retinal pigment epithelial cells via autocrine PDGF-receptor signaling.
    Authors: Bastiaans J, van Meurs J, van Holten-Neelen C, Nagtzaam N, van Hagen P, Chambers R, Hooijkaas H, Dik W
    Invest Ophthalmol Vis Sci, 2013;54(13):8306-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  45. Inactivation of nuclear factor-Y inhibits vascular smooth muscle cell proliferation and neointima formation.
    Authors: Silvestre-Roig C, Fernandez P, Esteban V, Pello O, Indolfi C, Rodriguez C, Rodriguez-Calvo R, Lopez-Maderuelo M, Bauriedel G, Hutter R, Fuster V, Ibanez B, Redondo J, Martinez-Gonzalez J, Andres V
    Arterioscler Thromb Vasc Biol, 2013;33(5):1036-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  46. Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells.
    Authors: Song S, Jung W, Kim K, Hong W, Kim H, Lee K, Lee K, Suh W
    Exp Cell Res, 2013;319(8):1136-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  47. Rapamycin inhibits smooth muscle cell proliferation and obstructive arteriopathy attributable to elastin deficiency.
    Authors: Li, Wei, Li, Qingle, Qin, Lingfeng, Ali, Rahmat, Qyang, Yibing, Tassabehji, May, Pober, Barbara, Sessa, William, Giordano, Frank J, Tellides, George
    Arterioscler Thromb Vasc Biol, 2013;33(5):1028-35.
    Species: Human
    Sample Type: Serum
    Application: Bioassay
  48. Novel role for p21-activated kinase 2 in thrombin-induced monocyte migration.
    Authors: Gadepalli R, Kotla S, Heckle M, Verma S, Singh N, Rao G
    J Biol Chem, 2013;288(43):30815-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  49. Src-mediated caveolin-1 phosphorylation affects the targeting of active Src to specific membrane sites.
    Authors: Gottlieb-Abraham, Efrat, Shvartsman, Dmitry E, Donaldson, John C, Ehrlich, Marcelo, Gutman, Orit, Martin, G Steven, Henis, Yoav I
    Mol Biol Cell, 2013;24(24):3881-95.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Type: Whole Cells
    Application: Bioassay
  50. ROS-mediated downregulation of MYPT1 in smooth muscle cells: a potential mechanism for the aberrant contractility in atherosclerosis.
    Authors: Cheng J, Cheng H, Tsai I, Jiang M
    Lab Invest, 2013;93(4):422-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  51. Cytotoxic effects of curcumin in human retinal pigment epithelial cells.
    Authors: Hollborn M, Chen R, Wiedemann P, Reichenbach A, Bringmann A, Kohen L
    PLoS ONE, 2013;8(3):e59603.
  52. Magnolol inhibits migration of vascular smooth muscle cells via cytoskeletal remodeling pathway to attenuate neointima formation.
    Authors: Karki R, Kim S, Kim D
    Exp Cell Res, 2013;319(20):3238-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  53. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012;30(7):1455-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  54. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  55. Neuropilin-1 signaling through p130Cas tyrosine phosphorylation is essential for growth factor-dependent migration of glioma and endothelial cells.
    Authors: Evans IM, Yamaji M, Britton G, Pellet-Many C, Lockie C, Zachary IC, Frankel P
    Mol. Cell. Biol., 2011;31(6):1174-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  56. Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4.
    Authors: Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A
    J. Biol. Chem., 2011;286(32):28097-110.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  57. Differentiation potential of human postnatal mesenchymal stem cells, mesoangioblasts, and multipotent adult progenitor cells reflected in their transcriptome and partially influenced by the culture conditions.
    Authors: Roobrouck VD, Clavel C, Jacobs SA, Ulloa-Montoya F, Crippa S, Sohni A, Roberts SJ, Luyten FP, Van Gool SW, Sampaolesi M, Delforge M, Luttun A, Verfaillie CM
    Stem Cells, 2011;29(5):871-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  58. Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
    Authors: Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV
    Oncogene, 2010;29(20):2938-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  59. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010;24(10):3782-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  60. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
    Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
    PLoS ONE, 2010;5(8):e12101.
    Species: Human
    Sample Type: Whole Cells
    Application: Expansion/Differentiation
  61. Thymic stromal lymphopoietin receptor-mediated IL-6 and CC/CXC chemokines expression in human airway smooth muscle cells: role of MAPKs (ERK1/2, p38, and JNK) and STAT3 pathways.
    Authors: Shan L, Redhu NS, Saleh A
    J. Immunol., 2010;184(12):7134-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  62. Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
    Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
    Biochem. J., 2010;427(1):29-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  63. KSHV-induced notch components render endothelial and mural cell characteristics and cell survival.
    Authors: Liu R, Li X, Tulpule A, Zhou Y, Scehnet JS, Zhang S, Lee JS, Chaudhary PM, Jung J, Gill PS
    Blood, 2010;115(4):887-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  64. Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.
    Authors: Wagsater D, Zhu C, Bjorck HM, Eriksson P
    Atherosclerosis, 2009;202(2):415-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  65. Imatinib mesylate and AMN107 inhibit PDGF-signaling in orbital fibroblasts: a potential treatment for Graves' ophthalmopathy.
    Authors: van Steensel L, Paridaens D, Schrijver B, Dingjan GM, van Daele PL, van Hagen PM, van den Bosch WA, Drexhage HA, Hooijkaas H, Dik WA
    Invest. Ophthalmol. Vis. Sci., 2009;50(7):3091-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  66. Transactivation of RON receptor tyrosine kinase by interaction with PDGF receptor beta during steady-state growth of human mesangial cells.
    Authors: Kobayashi T, Furukawa Y, Kikuchi J, Ito C, Miyata Y, Muto S, Tanaka A, Kusano E
    Kidney Int., 2009;75(11):1173-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  67. Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth.
    Authors: Tomanek RJ, Hansen HK, Christensen LP
    Arterioscler. Thromb. Vasc. Biol., 2008;28(7):1237-43.
    Species: Avian
    Sample Type: Whole Tissue
    Application: Bioassay
  68. Small molecule inhibitors of Hsp90 potently affect inflammatory disease pathways and exhibit activity in models of rheumatoid arthritis.
    Authors: Rice JW, Veal JM, Fadden RP, Barabasz AF, Partridge JM, Barta TE, Dubois LG, Huang KH, Mabbett SR, Silinski MA, Steed PM, Hall SE
    Arthritis Rheum., 2008;58(12):3765-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  69. Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage.
    Authors: Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC
    Exp. Cell Res., 2008;314(8):1721-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  70. Connective tissue growth factor as a mediator of intraocular fibrosis.
    Authors: He S, Chen Y, Khankan R, Barron E, Burton R, Zhu D, Ryan SJ, Oliver N, Hinton DR
    Invest. Ophthalmol. Vis. Sci., 2008;49(9):4078-88.
    Species: Rabbit
    Sample Type: In Vivo
    Application: In Vivo
  71. BIBF 1120: triple angiokinase inhibitor with sustained receptor blockade and good antitumor efficacy.
    Authors: Hilberg F, Roth GJ, Krssak M, Kautschitsch S, Sommergruber W, Tontsch-Grunt U, Garin-Chesa P, Bader G, Zoephel A, Quant J, Heckel A, Rettig WJ
    Cancer Res., 2008;68(12):4774-82.
  72. A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
    Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
    Exp. Cell Res., 2008;314(11):2176-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  73. Ang II-stimulated migration of vascular smooth muscle cells is dependent on LR11 in mice.
    Authors: Jiang M, Bujo H, Ohwaki K, Unoki H, Yamazaki H, Kanaki T, Shibasaki M, Azuma K, Harigaya K, Schneider WJ, Saito Y
    J. Clin. Invest., 2008;118(8):2733-46.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  74. Atrial-radiofrequency catheter ablation mediated targeting of mesenchymal stromal cells.
    Authors: Schweizer PA, Krause U, Becker R, Seckinger A, Bauer A, Hardt C, Eckstein V, Ho AD, Koenen M, Katus HA, Zehelein J
    Stem Cells, 2007;25(6):1546-51.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  75. C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
    Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
    Blood, 2007;109(10):4264-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  76. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  77. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  78. Bone morphogenetic protein 7 is elevated in patients with chronic liver disease and exerts fibrogenic effects on human hepatic stellate cells.
    Authors: Tacke F, Gabele E, Bataille F, Schwabe RF, Hellerbrand C, Klebl F, Straub RH, Luedde T, Manns MP, Trautwein C, Brenner DA, Scholmerich J, Schnabl B
    Dig. Dis. Sci., 2007;52(12):3404-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  79. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2006;107(5):1896-902.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  80. Multipotent adult progenitor cells from swine bone marrow.
    Authors: Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C
    Stem Cells, 2006;24(11):2355-66.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  81. Expression of the FUS-CHOP fusion protein in primary mesenchymal progenitor cells gives rise to a model of myxoid liposarcoma.
    Authors: Riggi N, Cironi L, Provero P, Suva ML, Stehle JC, Baumer K, Guillou L, Stamenkovic I
    Cancer Res., 2006;66(14):7016-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  82. Pitavastatin attenuates the PDGF-induced LR11/uPA receptor-mediated migration of smooth muscle cells.
    Authors: Jiang M, Bujo H, Zhu Y, Yamazaki H, Hirayama S, Kanaki T, Shibasaki M, Takahashi K, Schneider WJ, Saito Y
    Biochem. Biophys. Res. Commun., 2006;348(4):1367-77.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Bioassay
  83. Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways.
    Authors: Hunter I, Nixon GF
    J. Biol. Chem., 2006;281(45):34705-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  84. In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin.
    Authors: Wong AP, Nili N, Strauss BH
    Cardiovasc. Res., 2005;65(3):702-10.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Bioassay
  85. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  86. Differential gene expression and regulation of the bone morphogenetic protein antagonists follistatin and gremlin in normal and osteoarthritic human chondrocytes and synovial fibroblasts.
    Authors: Tardif G, Hum D, Pelletier JP, Boileau C, Ranger P, Martel-Pelletier J
    Arthritis Rheum., 2004;50(8):2521-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  87. Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads.
    Authors: Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI
    Am. J. Respir. Cell Mol. Biol., 2004;30(6):816-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  88. Dual roles of IL-4 in lung injury and fibrosis.
    Authors: Huaux F, Liu T, McGarry B, Ullenbruch M, Phan SH
    J. Immunol., 2003;170(4):2083-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  89. Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
    Am. J. Pathol., 2003;163(3):869-78.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Binding Assay
  90. Sef inhibits fibroblast growth factor signaling by inhibiting FGFR1 tyrosine phosphorylation and subsequent ERK activation.
    Authors: Kovalenko D, Yang X, Nadeau RJ, Harkins LK, Friesel R
    J. Biol. Chem., 2003;278(16):14087-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  91. Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release.
    Authors: Lacoste E, Martineau I, Gagnon G
    J. Periodontol., 2003;74(10):1498-507.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  92. A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
    Authors: Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
    J. Immunol., 2002;169(5):2653-61.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  93. Growth factors stimulate neointimal cells in vitro and increase the thickness of the neointima formed at the neck of porcine aneurysms treated by embolization.
    Authors: Desfaits AC, 48971, Raymond J, Muizelaar JP
    Cigarette smoke attenuates phagocytic ability of macrophages through down-regulating Milk fat globule-EGF factor 8 (MFG-E8) expressions, 2000;31(0):498-507.
    Species: Canine
    Sample Type: In Vivo
    Application: In Vivo
  94. Regulation of alpha(v)beta3 and alpha5beta1 integrin receptors by basic fibroblast growth factor and platelet-derived growth factor-BB in intrasynovial flexor tendon cells.
    Authors: Harwood FL, Goomer RS, Gelberman RH, Silva MJ, Amiel D
    Wound Repair Regen, 1999;7(5):381-8.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  95. Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.
    Authors: Akagi Y, Liu W, Zebrowski B, Xie K, Ellis LM
    Cancer Res., 1998;58(17):4008-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
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Animal-Free Recombinant Proteins
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Recombinant Human PDGF-BB, Animal-Free Protein

BA AFL220
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Average Rating: 4.4 (Based on 5 reviews)

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We have 5 reviews tested in 4 applications: Proof concept for delivery as therapeutic in a controlled delivery system, SDS-PAGE Control, Cell proliferation, Stem/Immune cell maintenance or differentiation .

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 Proof concept for delivery as therapeutic in a controlled delivery system Anonymous 02/12/2018
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Application Proof concept for delivery as therapeutic in a controlled delivery system
Reason for Rating Great for proof of concept, will see how it performs in a biological assay
SDS-PAGE Control Recombinant Human PDGF-BB Protein, CF 220-BB Array 220-BB
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 SDS-PAGE Control Anonymous 02/07/2018
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SDS-PAGE Control Recombinant Human PDGF-BB Protein, CF 220-BB
SDS-PAGE Control: Recombinant Human PDGF-BB Protein, CF [220-BB].

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 Cell proliferation Anonymous 09/11/2017
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 Stem/Immune cell maintenance or differentiation Anonymous 09/07/2017
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Application Stem/Immune cell maintenance or differentiation
Stem/Immune cell maintenance or differentiation  Recombinant Human PDGF-BB Protein, CF 220-BB Array 220-BB
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 Stem/Immune cell maintenance or differentiation Anonymous 07/22/2016
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Stem/Immune cell maintenance or differentiation  Recombinant Human PDGF-BB Protein, CF 220-BB
Stem/Immune cell maintenance or differentiation : Recombinant Human PDGF-BB Protein, CF [220-BB].

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Application Stem/Immune cell maintenance or differentiation

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