Recombinant Human Pentraxin 2/SAP His-tag Protein, CF

Catalog # Availability Size / Price Qty
1948-SAB-050
Recombinant Human Pentraxin 2/SAP His-tag Protein, CF Binding Activity
2 Images
Product Details
Citations (3)
FAQs
Reviews

Recombinant Human Pentraxin 2/SAP His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RllA/CD32a (R167) (Catalog # 1330-CD)  is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Pentraxin 2/SAP that produces 50% of the optimal binding response is 0.175-1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Pentraxin 2/SAP protein
His20-Val223, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
His20
Structure / Form
Noncovalently-linked homopentamer
Predicted Molecular Mass
25 kDa
SDS-PAGE
27-33 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1948-SAB

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl2 with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human Fc gamma  RIIA/CD32a (R167) (Catalog # 1330-CD) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human SAP binds with an ED50 of 0.175-1 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Pentraxin 2/SAP was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 27-33 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pentraxin 2/SAP

Pentraxin 2 (PTX2), also known as Serum Amyoid P Component (SAP), is a secreted serum glycoprotein that is a universal non-fibrillar component of amyloid deposits. These extracellular deposits of insoluble protein fibrils are the result of protein misfolding and can lead to tissue damage and disease (1, 2). PTX2 belongs to the pentaxin superfamily, whose members have the characteristic pentagonal discoid arrangement of five non-covalently bound subunits. Pentaxins bind to a variety of molecules in a calcium-dependent lectin-like manner through a pattern-recognition-binding site (1, 4, 5). Two subfamilies of pentaxins, the classical or short pentaxin subfamily that includes the serum C-reactive protein (CRP) and PTX2, and the fusion or long pentaxin subfamily whose members contain pentaxin-related
carboxyl-terminal halves, are known (1).

PTX2 and CRP share approximately 50% amino acid sequence identity (2, 5). They are produced and secreted by liver hepatocytes and circulates in plasma. Mouse PTX2 is a major acute-phase protein whose plasma concentrations increase dramatically during an acute phase response (2). In human where CRP is the major
acute-phase protein, the plasma concentration of human PTX2 remains relatively constant in response to tissue-damage (2, 5). The gene for PTX2 has been localized to human chromosome 1 where it is closely linked to the gene for CRP.

PTX2 associates ubiquitously with all amyloid deposits that are implicated in a diverse range of diseases including Alzheimer’s and prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). As a non-fibrillar component, PTX2 regulates the solubility of amyloid fibrils and protects them from degradation by proteolytic enzymes and phagocytic cells. In addition to its role in the pathogenesis of amyloidoses, PTX2 also has an important physiological function in innate immunity (8). It is an opsonin that interacts with all three types of human Fc gamma receptors that mediate phagocytosis by human polymorphonuclear leukocytes. It has been proposed that PTX2 may function as an opsonin for a variety of ligands including autoantigens, apoptotic cells, chromatin, DNA, and micro-organisms.

References
  1. Goodman, A. et al. (1996) Cytokine Growth Factor Rev. 7:191.
  2. Steel, D. and A. Whitehead (1994) Immunol. Today 15:81.
  3. Hirschfield, G.M. and P.N. Hawkins (2003) Int. J. Biochem. Cell Biol. 35:1608.
  4. Emsley, J. et al. (1994) Nature 367:338.
  5. Mantzouranis, E. et al. (1985) J. Biol. Chem. 260:7752.
  6. Botto, M. et al. (1997) Nature Medicine 3:855.
  7. Pepys, M. et al. (2002) Nature 417:254.
  8. Bharadwaj, D. et al. (2001) J. Immunol. 166:6735.
Long Name
Serum Amyloid P Component
Entrez Gene IDs
325 (Human); 20219 (Mouse); 29339 (Rat)
Alternate Names
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component

Citations for Recombinant Human Pentraxin 2/SAP His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1
    Authors: Nobutaka Wakamiya
    Biochim. Biophys. Acta, 2016;0(0):.
    Species: N/A
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Pentraxin-2 suppresses c-Jun/AP-1 signaling to inhibit progressive fibrotic disease
    JCI Insight, 2016;1(20):e87446.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Fc receptor engagement mediates differentiation of cardiac fibroblast precursor cells.
    Authors: Haudek SB, Trial J, Xia Y, Gupta D, Pilling D, Entman ML
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(29):10179-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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