Recombinant Human Proprotein Convertase 9/PCSK9 Protein, CF

Catalog # Availability Size / Price Qty
3888-SE-010
Proprotein Convertase 9/PCSK9 Protein Bioactivity
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Product Details
Citations (1)
FAQs
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Reviews (1)

Recombinant Human Proprotein Convertase 9/PCSK9 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LDL R (Catalog # 2148-LD) is coated at 2 µg/mL, Recombinant Human Proprotein Convertase 9/PCSK9 binds with an ED50 of 70-420 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Proprotein Convertase 9/PCSK9 protein
Gln31-Gln152 & Ser153-Gln692, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Ser153 (mature form); No results obtained for pro domain, Gln31 inferred from enzymatic pyroglutamate treatment revealing Glu32
Structure / Form
Mature form & pro domain
Predicted Molecular Mass
14 kDa & 59 kDa
SDS-PAGE
19 kDa & 66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3888-SE

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Image

Binding Activity Binding Activity View Larger

When Recombinant Human LDL R (Catalog # 2148-LD) is coated at 2 μg/mL, Recombinant Human Proprotein Convertase9/
PCSK9 (Catalog # 3888-SE) binds with an ED50 of 70‑420 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: Proprotein Convertase 9/PCSK9

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (residues 1 to 30), a pro peptide (residue 31 to 152), and a mature chain (residues 153 to 692) that contains a serine protease domain (residues 161 to 431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non‑covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

References
  1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
  2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.
Entrez Gene IDs
255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)
Alternate Names
EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9

Citation for Recombinant Human Proprotein Convertase 9/PCSK9 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proprotein convertase subtilisin kexin type 9 promotes intestinal overproduction of triglyceride-rich apolipoprotein B lipoproteins through both low-density lipoprotein receptor-dependent and -independent mechanisms.
    Authors: Rashid S, Tavori H, Brown P, Linton M, He J, Giunzioni I, Fazio S
    Circulation, 2014;130(5):431-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Proprotein Convertase 9/PCSK9 Protein, CF
By Anonymous on 04/25/2019
Application: Binding assay/Protein-protein interaction