Reagent Diluent Concentrate 2 Summary
INTENDED USE & DESCRIPTION
Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.
For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
- The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information.
Citations for Reagent Diluent Concentrate 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells
Authors: F Zilli, P Marques Ra, P Auf der Ma, C Jehanno, A Sethi, MM Coissieux, T Eichlisber, L Sauteur, A Rouchon, L Bonapace, J Pinto Cout, R Rad, MR Jensen, A Banfi, MB Stadler, M Bentires-A
Embo Molecular Medicine, 2021;0(0):e13162. 2021
bFGF blockade reduces intraplaque angiogenesis and macrophage infiltration in atherosclerotic vein graft lesions in ApoE3*Leiden mice
Authors: L Parma, HAB Peters, TJ Sluiter, KH Simons, P Lazzari, MR de Vries, PHA Quax
Sci Rep, 2020;10(1):15968. 2020
Differentially expressed circular RNAs in a murine asthma model
Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
Molecular Medicine Reports, 2020;22(6):5412-5422. 2020
Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
Int J Nanomedicine, 2019;14(0):6117-6131. 2019
Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
J Clin Med, 2019;8(7):. 2019
Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
J Immunol Res, 2019;2019(0):1845128. 2019
Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
Sci Rep, 2018;8(1):10328. 2018
Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
Planta, 2018;0(0):. 2018
Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
Cell Transplant, 2018;0(0):9636897187922. 2018
Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
Authors: M Oleszowsky, MF Seidel
Biomed Res Int, 2018;2018(0):8286067. 2018
Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
Oncol Lett, 2017;13(5):3269-3276. 2017
Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
Oncol Lett, 2017;13(6):4995-5001. 2017
IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
JCI Insight, 2017;2(10):. 2017
Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
Nat Commun, 2014;5(0):3779. 2014
Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
Mol Neurodegener, 2014;9(0):22. 2014
Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
Authors: Litzlbauer P, Weber K, Mueller R
Cytokine, 2013;66(1):54-9. 2013
A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
PLoS ONE, 2012;7(6):e39036. 2012
Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
Authors: Reddy PB, Sehrawat S, Suryawanshi A
J. Immunol., 2011;187(11):5745-55. 2011
Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
Authors: King GD, Muhammad AK, Larocque D
Mol. Ther., 2011;19(10):1793-801. 2011
Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010;5(2):e9246. 2010
First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2010;33(3):664-9. 2010
Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
Authors: Derycke MS, Pambuccian SE, Gilks CB
Am. J. Clin. Pathol., 2010;134(5):835-45. 2010
Normal ranges of angiogenesis regulatory proteins in human platelets.
Authors: Peterson JE, Zurakowski D, Italiano JE
Am. J. Hematol., 2010;85(7):487-93. 2010
Nanobacteria are mineralo fetuin complexes.
Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
PLoS Pathog., 2008;4(2):e41. 2008
A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
Clin. Exp. Allergy, 2008;38(4):594-601. 2008
Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
Cancer Res., 2007;67(15):7458-66. 2007
Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
J. Neurochem., 2006;96(6):1560-71. 2006
Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains.
Authors: Harlan S, Ostroski R, Coskun T, Yantis L, Breyer M, Heuer J
Am J Physiol Regul Integr Comp Physiol, 0;309(5):R467-74. 0
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Reviews for Reagent Diluent Concentrate 2
Average Rating: 5 (Based on 25 Reviews)
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Effective blocking reagent for FACs binding assays. The multiple vials make them easy for one time use on days of assays without having to worry about contaminating a large stock.
Always produces great results with every duoset I have used. For me, this is usually the only additional reagent I need outside the Ancillary Reagent Kit 2 that I usually purchase
I have been using the this for several ELISA assays and has been working with no problem
excellent blocker, we used 7 kits so far. Works well for serum ELISA's
I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate
I use RD to make standard curves
Used the reagent diluent for assay and it works perfect has very little to no background
Used for Duoset ELISA