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Reagent Diluent Concentrate 2

R&D Systems | Catalog # DY995

10X, 5 x 21 mL
R&D Systems
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Key Product Details

Applications

ELISA

Product Summary for Reagent Diluent Concentrate 2

INTENDED USE & DESCRIPTION

Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.

REAGENT PREPARATION

For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information. 

Product Documents for Reagent Diluent Concentrate 2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Reagent Diluent Concentrate 2

For research use only

Citations for Reagent Diluent Concentrate 2

Customer Reviews for Reagent Diluent Concentrate 2 (31)

4.9 out of 5
31 Customer Ratings
5 Stars
94%
4 Stars
6%
3 Stars
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1 Stars
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  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/02/2026
    Used in Angiogenin Duoset ELISA to test conditioned media of adipose tissue and cancer spheroids
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/02/2026
    MCF7 and adipose tissue conditioned media analysed with IL-8 ELISA Duoset
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 02/18/2025
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 07/01/2024
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 05/21/2024
    worked well with DY2780.
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 05/23/2022
    Great for use in ELISAs. Consistent product and convenient to use.
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 09/23/2021
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/31/2021
    Effective blocking reagent for FACs binding assays. The multiple vials make them easy for one time use on days of assays without having to worry about contaminating a large stock.
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Hon Wai Koon
    Verified Customer | Posted 12/01/2020
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 11/22/2020
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 09/14/2020
    Always produces great results with every duoset I have used. For me, this is usually the only additional reagent I need outside the Ancillary Reagent Kit 2 that I usually purchase
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 08/17/2020
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/17/2020
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/10/2020
  • Reagent Diluent Concentrate 2
    Name: Tahmineh Safaie
    Verified Customer | Posted 12/23/2019
    I have been using the this for several ELISA assays and has been working with no problem
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 09/23/2019
    excellent blocker, we used 7 kits so far. Works well for serum ELISA's
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 03/28/2019
    I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Marry Tahani
    Verified Customer | Posted 11/20/2018
    Gives really good linearity.
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 08/17/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 07/17/2018
    Great blocker
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 06/15/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 04/30/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 04/11/2018
  • Reagent Diluent Concentrate 2
    Name: Leslie Priddy
    Verified Customer | Posted 04/03/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 02/06/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/19/2018
    I use RD to make standard curves
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/19/2018
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Anonymous
    Verified Customer | Posted 07/19/2017
    Used the reagent diluent for assay and it works perfect has very little to no background
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Shubhanchi Nigam
    Verified Customer | Posted 07/14/2017
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Anonymous
    Verified Customer | Posted 07/06/2017
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Anonymous
    Verified Customer | Posted 03/14/2017
    Used for Duoset ELISA

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