Reagent Diluent Concentrate 2

10X, 5 x 21 mL
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Reagent Diluent Concentrate 2
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Product Details
Citations (28)
Supplemental Products
Reviews (25)

Reagent Diluent Concentrate 2 Summary


Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.


Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.


For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.


  • The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information. 


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

Citations for Reagent Diluent Concentrate 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells
    Authors: F Zilli, P Marques Ra, P Auf der Ma, C Jehanno, A Sethi, MM Coissieux, T Eichlisber, L Sauteur, A Rouchon, L Bonapace, J Pinto Cout, R Rad, MR Jensen, A Banfi, MB Stadler, M Bentires-A
    Embo Molecular Medicine, 2021;0(0):e13162.  2021
  2. bFGF blockade reduces intraplaque angiogenesis and macrophage infiltration in atherosclerotic vein graft lesions in ApoE3*Leiden mice
    Authors: L Parma, HAB Peters, TJ Sluiter, KH Simons, P Lazzari, MR de Vries, PHA Quax
    Sci Rep, 2020;10(1):15968.  2020
  3. Differentially expressed circular RNAs in a murine asthma model
    Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
    Molecular Medicine Reports, 2020;22(6):5412-5422.  2020
  4. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019;14(0):6117-6131.  2019
  5. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019;8(7):.  2019
  6. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
    Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
    J Immunol Res, 2019;2019(0):1845128.  2019
  7. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018;8(1):10328.  2018
  8. Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
    Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
    Planta, 2018;0(0):.  2018
  9. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
    Cell Transplant, 2018;0(0):9636897187922.  2018
  10. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018;2018(0):8286067.  2018
  11. Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
    Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
    Oncol Lett, 2017;13(5):3269-3276.  2017
  12. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017;13(6):4995-5001.  2017
  13. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017;2(10):.  2017
  14. Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
    Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
    Nat Commun, 2014;5(0):3779.  2014
  15. Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
    Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
    Mol Neurodegener, 2014;9(0):22.  2014
  16. Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
    Authors: Litzlbauer P, Weber K, Mueller R
    Cytokine, 2013;66(1):54-9.  2013
  17. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036.  2012
  18. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011;187(11):5745-55.  2011
  19. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011;19(10):1793-801.  2011
  20. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246.  2010
  21. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2010;33(3):664-9.  2010
  22. Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
    Authors: Derycke MS, Pambuccian SE, Gilks CB
    Am. J. Clin. Pathol., 2010;134(5):835-45.  2010
  23. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010;85(7):487-93.  2010
  24. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41.  2008
  25. A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
    Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
    Clin. Exp. Allergy, 2008;38(4):594-601.  2008
  26. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007;67(15):7458-66.  2007
  27. Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
    Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
    J. Neurochem., 2006;96(6):1560-71.  2006
  28. Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains.
    Authors: Harlan S, Ostroski R, Coskun T, Yantis L, Breyer M, Heuer J
    Am J Physiol Regul Integr Comp Physiol, 0;309(5):R467-74.  0


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Reviews for Reagent Diluent Concentrate 2

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Reagent Diluent Concentrate 2
By Anonymous on 09/23/2021

Reagent Diluent Concentrate 2
By Anonymous on 01/31/2021

Effective blocking reagent for FACs binding assays. The multiple vials make them easy for one time use on days of assays without having to worry about contaminating a large stock.

Reagent Diluent Concentrate 2
By Anonymous on 12/01/2020

Reagent Diluent Concentrate 2
By Anonymous on 11/22/2020

Reagent Diluent Concentrate 2
By Anonymous on 09/14/2020

Always produces great results with every duoset I have used. For me, this is usually the only additional reagent I need outside the Ancillary Reagent Kit 2 that I usually purchase

Reagent Diluent Concentrate 2
By Anonymous on 08/17/2020

Reagent Diluent Concentrate 2
By Anonymous on 01/17/2020

Reagent Diluent Concentrate 2
By Anonymous on 01/10/2020

Reagent Diluent Concentrate 2
By Anonymous on 12/23/2019

I have been using the this for several ELISA assays and has been working with no problem

Reagent Diluent Concentrate 2
By Anonymous on 09/23/2019

excellent blocker, we used 7 kits so far. Works well for serum ELISA's

Reagent Diluent Concentrate 2
By Anonymous on 03/28/2019

I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate

Reagent Diluent Concentrate 2
By Anonymous on 11/20/2018

Gives really good linearity.

Reagent Diluent Concentrate 2
By Anonymous on 08/17/2018

Reagent Diluent Concentrate 2
By Anonymous on 07/17/2018

Great blocker

Reagent Diluent Concentrate 2
By Anonymous on 06/15/2018

Reagent Diluent Concentrate 2
By Anonymous on 04/30/2018

Reagent Diluent Concentrate 2
By Anonymous on 04/11/2018

Reagent Diluent Concentrate 2
By Anonymous on 04/03/2018

Reagent Diluent Concentrate 2
By Anonymous on 02/06/2018

Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018

I use RD to make standard curves

Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/19/2017

Used the reagent diluent for assay and it works perfect has very little to no background

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/14/2017

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/06/2017

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 03/14/2017

Used for Duoset ELISA