Reagent Diluent Concentrate 2

10X, 5 x 21 mL
Catalog # Availability Size / Price Qty
DY995
Reagent Diluent Concentrate 2
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (19)

Reagent Diluent Concentrate 2 Summary

INTENDED USE & DESCRIPTION

Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.

REAGENT PREPARATION

For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

Data Example

Reagent Diluent Concentrate 2

Reagents Provided

Other Reagents Provided

Citations for Reagent Diluent Concentrate 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019;14(0):6117-6131.  2019
  2. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019;8(7):.  2019
  3. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
    Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
    J Immunol Res, 2019;2019(0):1845128.  2019
  4. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018;8(1):10328.  2018
  5. Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
    Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
    Planta, 2018;0(0):.  2018
  6. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
    Cell Transplant, 2018;0(0):9636897187922.  2018
  7. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018;2018(0):8286067.  2018
  8. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017;13(6):4995-5001.  2017
  9. Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
    Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
    Oncol Lett, 2017;13(5):3269-3276.  2017
  10. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017;2(10):.  2017
  11. Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
    Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
    Mol Neurodegener, 2014;9(0):22.  2014
  12. Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
    Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
    Nat Commun, 2014;5(0):3779.  2014
  13. Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
    Authors: Litzlbauer P, Weber K, Mueller R
    Cytokine, 2013;66(1):54-9.  2013
  14. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036.  2012
  15. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011;187(11):5745-55.  2011
  16. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011;19(10):1793-801.  2011
  17. Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
    Authors: Derycke MS, Pambuccian SE, Gilks CB
    Am. J. Clin. Pathol., 2010;134(5):835-45.  2010
  18. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246.  2010
  19. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2010;33(3):664-9.  2010
  20. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010;85(7):487-93.  2010
  21. A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
    Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
    Clin. Exp. Allergy, 2008;38(4):594-601.  2008
  22. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41.  2008
  23. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007;67(15):7458-66.  2007
  24. Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
    Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
    J. Neurochem., 2006;96(6):1560-71.  2006

FAQs

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Reviews for Reagent Diluent Concentrate 2

Average Rating: 4.9 (Based on 19 Reviews)

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Reagent Diluent Concentrate 2
By Anonymous on 01/17/2020
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Reagent Diluent Concentrate 2
By Anonymous on 01/10/2020
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Reagent Diluent Concentrate 2
By Anonymous on 12/23/2019
Application:

I have been using the this for several ELISA assays and has been working with no problem


Reagent Diluent Concentrate 2
By Anonymous on 09/23/2019
Application:

excellent blocker, we used 7 kits so far. Works well for serum ELISA's


Reagent Diluent Concentrate 2
By Anonymous on 03/28/2019
Application:

I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate


Reagent Diluent Concentrate 2
By Anonymous on 11/20/2018
Application:

Gives really good linearity.


Reagent Diluent Concentrate 2
By Anonymous on 08/17/2018
Application:

Reagent Diluent Concentrate 2
By Anonymous on 07/17/2018
Application:

Great blocker


Reagent Diluent Concentrate 2
By Anonymous on 06/15/2018
Application:

Reagent Diluent Concentrate 2
By Anonymous on 04/30/2018
Application:

Reagent Diluent Concentrate 2
By Anonymous on 04/11/2018
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Reagent Diluent Concentrate 2
By Anonymous on 04/03/2018
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Reagent Diluent Concentrate 2
By Anonymous on 02/06/2018
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Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018
Application:

I use RD to make standard curves


Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018
Application:

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/19/2017
Application:

Used the reagent diluent for assay and it works perfect has very little to no background


Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/14/2017
Application:

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/06/2017
Application:

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 03/14/2017
Application:

Used for Duoset ELISA