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Reagent Diluent Concentrate 2

R&D Systems | Catalog # DY995

10X, 5 x 21 mL
R&D Systems
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Key Product Details

Applications

ELISA

Product Summary for Reagent Diluent Concentrate 2

INTENDED USE & DESCRIPTION

Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.

REAGENT PREPARATION

For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information. 

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for Reagent Diluent Concentrate 2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Reagent Diluent Concentrate 2

For research use only

Citations for Reagent Diluent Concentrate 2

Customer Reviews for Reagent Diluent Concentrate 2 (31)

4.9 out of 5
31 Customer Ratings
5 Stars
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4 Stars
6%
3 Stars
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  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/02/2026
    Used in Angiogenin Duoset ELISA to test conditioned media of adipose tissue and cancer spheroids
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/02/2026
    MCF7 and adipose tissue conditioned media analysed with IL-8 ELISA Duoset
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 02/18/2025
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 07/01/2024
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 05/21/2024
    worked well with DY2780.
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 05/23/2022
    Great for use in ELISAs. Consistent product and convenient to use.
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 09/23/2021
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/31/2021
    Effective blocking reagent for FACs binding assays. The multiple vials make them easy for one time use on days of assays without having to worry about contaminating a large stock.
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Hon Wai Koon
    Verified Customer | Posted 12/01/2020
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 11/22/2020
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 09/14/2020
    Always produces great results with every duoset I have used. For me, this is usually the only additional reagent I need outside the Ancillary Reagent Kit 2 that I usually purchase
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 08/17/2020
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/17/2020
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/10/2020
  • Reagent Diluent Concentrate 2
    Name: Tahmineh Safaie
    Verified Customer | Posted 12/23/2019
    I have been using the this for several ELISA assays and has been working with no problem
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 09/23/2019
    excellent blocker, we used 7 kits so far. Works well for serum ELISA's
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 03/28/2019
    I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Marry Tahani
    Verified Customer | Posted 11/20/2018
    Gives really good linearity.
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 08/17/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 07/17/2018
    Great blocker
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 06/15/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 04/30/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 04/11/2018
  • Reagent Diluent Concentrate 2
    Name: Leslie Priddy
    Verified Customer | Posted 04/03/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 02/06/2018
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/19/2018
    I use RD to make standard curves
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2
    Name: Anonymous
    Verified Customer | Posted 01/19/2018
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Anonymous
    Verified Customer | Posted 07/19/2017
    Used the reagent diluent for assay and it works perfect has very little to no background
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Shubhanchi Nigam
    Verified Customer | Posted 07/14/2017
    Reagent Diluent Concentrate 2 DY995
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Anonymous
    Verified Customer | Posted 07/06/2017
  • Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
    Name: Anonymous
    Verified Customer | Posted 03/14/2017
    Used for Duoset ELISA

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