Reagent Diluent Concentrate 2
10X, 5 x 21 mL
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Reagent Diluent Concentrate 2 Summary
INTENDED USE & DESCRIPTION
Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.
REAGENT PREPARATION
For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
- The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information.
Specifications
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA
Product Datasheets
Citations for Reagent Diluent Concentrate 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells
Authors: Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H;
Cells 2023-08-19
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Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
Authors: MJ Rodrigo, I Bravo-Osun, M Subias, A Montolío, J Cegoñino, T Martinez-R, S Mendez-Mar, A Aragón-Nav, D Garcia-Her, LE Pablo, R Herrero-Va, AP Del Paloma, E Garcia-Mar
Scientific Reports, 2022-11-30;12(1):20622. 2022-11-30
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Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
Authors: H Kim, B Jang, YJ Kim, JC Choi
International Journal of Molecular Sciences, 2022-09-11;23(18):. 2022-09-11
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Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
Nature, 2021-11-03;0(0):. 2021-11-03
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Topological isolation of developmental regulators in mammalian genomes
Authors: HJ Wu, A Landshamme, EK Stamenova, A Bolondi, H Kretzmer, A Meissner, F Michor
Nature Communications, 2021-08-12;12(1):4897. 2021-08-12
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The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells
Authors: F Zilli, P Marques Ra, P Auf der Ma, C Jehanno, A Sethi, MM Coissieux, T Eichlisber, L Sauteur, A Rouchon, L Bonapace, J Pinto Cout, R Rad, MR Jensen, A Banfi, MB Stadler, M Bentires-A
Embo Molecular Medicine, 2021-03-10;0(0):e13162. 2021-03-10
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Detection of Plasmodium falciparum malaria in 1�h using a simplified enzyme-linked immunosorbent assay
Authors: E de la Sern, K Arias-Alpí, LN Borgheti-C, A Sanchez-Ca, E Sulleiro, F Zarzuela, P Bosch-Nico, F Salvador, I Molina, M Ramírez, X Fernàndez-, A Sánchez-Mo, E Baldrich
Analytica chimica acta, 2021-01-30;1152(0):338254. 2021-01-30
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Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
Authors: L Kang, S Smith, C Wang
Journal of colloid and interface science, 2020-12-24;588(0):101-109. 2020-12-24
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Differentially expressed circular RNAs in a murine asthma model
Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
Molecular Medicine Reports, 2020-10-20;22(6):5412-5422. 2020-10-20
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bFGF blockade reduces intraplaque angiogenesis and macrophage infiltration in atherosclerotic vein graft lesions in ApoE3*Leiden mice
Authors: L Parma, HAB Peters, TJ Sluiter, KH Simons, P Lazzari, MR de Vries, PHA Quax
Sci Rep, 2020-09-29;10(1):15968. 2020-09-29
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Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis
Authors: J Vullings, JP Vago, CEJ Waterborg, RM Thurlings, MI Koenders, PLEM van Lent, PM van der Kr, FA Amaral, FAJ van de Loo
J Immunol Res, 2020-09-11;2020(0):9690832. 2020-09-11
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BMP9 signaling promotes the normalization of tumor blood vessels
Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
Oncogene, 2020-02-10;0(0):. 2020-02-10
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Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
Int J Nanomedicine, 2019-08-01;14(0):6117-6131. 2019-08-01
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Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
J Clin Med, 2019-07-15;8(7):. 2019-07-15
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Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
J Immunol Res, 2019-04-11;2019(0):1845128. 2019-04-11
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Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
Cell Transplant, 2018-09-25;0(0):9636897187922. 2018-09-25
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Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
Sci Rep, 2018-07-09;8(1):10328. 2018-07-09
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Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
Authors: M Oleszowsky, MF Seidel
Biomed Res Int, 2018-02-01;2018(0):8286067. 2018-02-01
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Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
Planta, 2018-01-08;0(0):. 2018-01-08
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IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
JCI Insight, 2017-05-18;2(10):. 2017-05-18
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Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
Oncol Lett, 2017-04-21;13(6):4995-5001. 2017-04-21
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Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
Oncol Lett, 2017-03-13;13(5):3269-3276. 2017-03-13
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Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains.
Authors: Harlan S, Ostroski R, Coskun T, Yantis L, Breyer M, Heuer J
Am J Physiol Regul Integr Comp Physiol, 2015-06-24;309(5):R467-74. 2015-06-24
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Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
Mol Neurodegener, 2014-06-06;9(0):22. 2014-06-06
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Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
Nat Commun, 2014-04-30;5(0):3779. 2014-04-30
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Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
Authors: Litzlbauer P, Weber K, Mueller R
Cytokine, 2014-01-14;66(1):54-9. 2014-01-14
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A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
PLoS ONE, 2012-06-20;7(6):e39036. 2012-06-20
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Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
Authors: Reddy PB, Sehrawat S, Suryawanshi A
J. Immunol., 2011-10-21;187(11):5745-55. 2011-10-21
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Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
Authors: King GD, Muhammad AK, Larocque D
Mol. Ther., 2011-04-19;19(10):1793-801. 2011-04-19
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Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
Authors: Derycke MS, Pambuccian SE, Gilks CB
Am. J. Clin. Pathol., 2010-11-01;134(5):835-45. 2010-11-01
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Normal ranges of angiogenesis regulatory proteins in human platelets.
Authors: Peterson JE, Zurakowski D, Italiano JE
Am. J. Hematol., 2010-07-01;85(7):487-93. 2010-07-01
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Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010-02-16;5(2):e9246. 2010-02-16
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First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2009-12-10;33(3):664-9. 2009-12-10
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A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
Clin. Exp. Allergy, 2008-02-25;38(4):594-601. 2008-02-25
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Nanobacteria are mineralo fetuin complexes.
Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
PLoS Pathog., 2008-02-08;4(2):e41. 2008-02-08
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Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
Cancer Res., 2007-08-01;67(15):7458-66. 2007-08-01
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Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
J. Neurochem., 2006-02-08;96(6):1560-71. 2006-02-08
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Reviews for Reagent Diluent Concentrate 2
Average Rating: 5 (Based on 26 Reviews)
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Reagent Diluent Concentrate 2
By Anonymous
on 05/23/2022
Great for use in ELISAs. Consistent product and convenient to use.
Reagent Diluent Concentrate 2
By Anonymous
on 09/23/2021
Reagent Diluent Concentrate 2
By Anonymous
on 01/31/2021
Effective blocking reagent for FACs binding assays. The multiple vials make them easy for one time use on days of assays without having to worry about contaminating a large stock.
Reagent Diluent Concentrate 2
By Hon Wai Koon
on 12/01/2020
Reagent Diluent Concentrate 2
By Anonymous
on 11/22/2020
Reagent Diluent Concentrate 2
By Anonymous
on 09/14/2020
Always produces great results with every duoset I have used. For me, this is usually the only additional reagent I need outside the Ancillary Reagent Kit 2 that I usually purchase
Reagent Diluent Concentrate 2
By Anonymous
on 08/17/2020
Reagent Diluent Concentrate 2
By Anonymous
on 01/17/2020
Reagent Diluent Concentrate 2
By Anonymous
on 01/10/2020
Reagent Diluent Concentrate 2
By Tahmineh Safaie
on 12/23/2019
I have been using the this for several ELISA assays and has been working with no problem
Reagent Diluent Concentrate 2
By Anonymous
on 09/23/2019
excellent blocker, we used 7 kits so far. Works well for serum ELISA's
Reagent Diluent Concentrate 2
By Anonymous
on 03/28/2019
I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate
Reagent Diluent Concentrate 2
By Marry Tahani
on 11/20/2018
Gives really good linearity.
Reagent Diluent Concentrate 2
By Anonymous
on 08/17/2018
Reagent Diluent Concentrate 2
By Anonymous
on 07/17/2018
Great blocker
Reagent Diluent Concentrate 2
By Anonymous
on 06/15/2018
Reagent Diluent Concentrate 2
By Anonymous
on 04/30/2018
Reagent Diluent Concentrate 2
By Anonymous
on 04/11/2018
Reagent Diluent Concentrate 2
By Leslie Priddy
on 04/03/2018
Reagent Diluent Concentrate 2
By Anonymous
on 02/06/2018
Reagent Diluent Concentrate 2
By Anonymous
on 01/19/2018
I use RD to make standard curves
Reagent Diluent Concentrate 2
By Anonymous
on 01/19/2018
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous
on 07/19/2017
Used the reagent diluent for assay and it works perfect has very little to no background
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Shubhanchi Nigam
on 07/14/2017
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous
on 07/06/2017
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous
on 03/14/2017
Used for Duoset ELISA
