Reagent Diluent Concentrate 2

10X, 5 x 21 mL
Catalog # Availability Size / Price Qty
DY995
Reagent Diluent Concentrate 2
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Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (13)

Reagent Diluent Concentrate 2 Summary

INTENDED USE & DESCRIPTION

Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.

REAGENT PREPARATION

For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information. 

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Reagent Diluent Concentrate 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018;2018(0):8286067.  2018
  2. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018;8(1):10328.  2018
  3. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017;13(6):4995-5001.  2017
  4. Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
    Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
    Oncol Lett, 2017;13(5):3269-3276.  2017
  5. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017;2(10):.  2017
  6. Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
    Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
    Nat Commun, 2014;5(0):3779.  2014
  7. Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
    Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
    Mol Neurodegener, 2014;9(0):22.  2014
  8. Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
    Authors: Litzlbauer P, Weber K, Mueller R
    Cytokine, 2013;66(1):54-9.  2013
  9. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036.  2012
  10. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011;19(10):1793-801.  2011
  11. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011;187(11):5745-55.  2011
  12. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2010;33(3):664-9.  2010
  13. Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
    Authors: Derycke MS, Pambuccian SE, Gilks CB
    Am. J. Clin. Pathol., 2010;134(5):835-45.  2010
  14. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246.  2010
  15. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010;85(7):487-93.  2010
  16. A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
    Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
    Clin. Exp. Allergy, 2008;38(4):594-601.  2008
  17. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41.  2008
  18. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007;67(15):7458-66.  2007
  19. Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
    Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
    J. Neurochem., 2006;96(6):1560-71.  2006

FAQs

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Reviews for Reagent Diluent Concentrate 2

Average Rating: 4.9 (Based on 13 Reviews)

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Reagent Diluent Concentrate 2
By Anonymous on 08/17/2018

Reagent Diluent Concentrate 2
By Anonymous on 07/17/2018

Great blocker


Reagent Diluent Concentrate 2
By Anonymous on 06/15/2018

Reagent Diluent Concentrate 2
By Anonymous on 04/30/2018

Reagent Diluent Concentrate 2
By Anonymous on 04/11/2018

Reagent Diluent Concentrate 2
By Anonymous on 04/03/2018

Reagent Diluent Concentrate 2
By Anonymous on 02/06/2018

Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018

I use RD to make standard curves


Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/19/2017

Used the reagent diluent for assay and it works perfect has very little to no background


Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/14/2017

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/06/2017

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 03/14/2017

Used for Duoset ELISA