Recombinant Human Ret Fc Chimera Protein, CF

R&D Systems | Catalog # 9986-CR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Ret Fc Chimera Protein (9986-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P07949

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Ret Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Ret protein
Human Ret
(Leu29-Arg635)
Accession # P07949		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

94 kDa

SDS-PAGE

120 - 140 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Ret Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.02-0.12 μg/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR).

Scientific Data Images for Recombinant Human Ret Fc Chimera Protein, CF

Recombinant Human Ret Fc Chimera Protein Binding Activity

Recombinant Human Ret Fc Chimera Protein Binding Activity

When Recombinant Human Ret Fc Chimera (Catalog # 9986-CR) is immobilized at 1 µg/mL, Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.02-0.12 µg/mL in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera (Catalog # 714-GR).
Recombinant Human Ret Fc Chimera Protein SDS-PAGE

Recombinant Human Ret Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Ret Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-140 kDa and 240-280 kDa, respectively.

Formulation, Preparation, and Storage

9986-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and in the adult animal. The GDNF family members (GDNF, Neurturin, Artemin and Persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1-4). GFR alpha -1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, Neurturin, Artemin and Persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the Ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung's disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1-4).

References

  1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
  4. Baloh, R. et al. (1998) Neuron 21:1291.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Alternate Names

CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence

Entrez Gene IDs

5979 (Human); 19713 (Mouse)

Gene Symbol

RET

UniProt

P07949

Additional Ret Products

Product Documents for Recombinant Human Ret Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ret Fc Chimera Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers
GDNF Family

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