Recombinant Human SCF (Mammalian-expressed) Protein

Carrier Free

Catalog # Availability Size / Price Qty
7466-SC-010/CF
7466-SC-025/CF
7466-SC-100/CF

With Carrier

Catalog # Availability Size / Price Qty
7466-SC-010
7466-SC-500
7466-SC-01M
Recombinant Human SCF (Mammalian-expressed) Protein Bioactivity
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Product Details
Citations (3)
FAQs
Reviews

Recombinant Human SCF (Mammalian-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1-5 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human SCF/c-kit Ligand protein
Glu26-Ala189
Accession #
N-terminal Sequence
Analysis
Glu26
Predicted Molecular Mass
18.5 kDa (monomer)
SDS-PAGE
24-30 kDa, reducing conditions

Product Datasheets

7466-SC (with carrier)

7466-SC/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7466-SC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7466-SC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human SCF (Mammalian-expressed) Protein Bioactivity View Larger

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 1-5 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c‑kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O‑linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel‑dickie mutant mice (11, 12). An alternatively spliced isoform of human SCF exists that lacks 28 aa that encompass the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

References
  1. Ashman, L.K., 1999, Int. J. Biochem. Cell Biol. 31:1037.
  2. Sette, C. et al., 2000, Int. J. Dev. Biol. 44:599.
  3. Yoshida, H. et al., 2001, J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Erlandsson, A. et al., 2004, Exp. Cell Res. 301:201.
  5. Kapur, R. et al., 2002, Blood 100:1287.
  6. Wang, C.-H. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27:540.
  7. Bashamboo, A. et al., 2006, J. Cell Sci. 119:3039.
  8. Reber, L. et al., 2006, Eur. J. Pharmacol. 533:327.
  9. Martin, F.H. et al., 1990, Cell 63:203.
  10. Arakawa, T. et al., 1991, J. Biol. Chem. 266:18942.
  11. Majumdar, M.K. et al., 1994, J. Biol. Chem. 269:1237.
  12. Brannan, C.I. et al., 1991, Proc. Natl. Acad. Sci. USA 88:4671.
  13. 13. Anderson, D.M. et al., 1991, Cell Growth Differ. 2:373.
  14. 14. Lemmon, M.A. et al., 1997, J. Biol. Chem. 272:6311.
  15. 15. Kanellakis, P. et al., 2006, Cardiovasc. Res. 70:117.
Long Name
Stem Cell Factor
Entrez Gene IDs
4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names
c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

Citations for Recombinant Human SCF (Mammalian-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
    J. Clin. Invest., 2019;130(0):2555-2570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Neutralizing negative epigenetic regulation by HDAC5 enhances human haematopoietic stem cell homing and engraftment
    Authors: X Huang, B Guo, S Liu, J Wan, HE Broxmeyer
    Nat Commun, 2018;9(1):2741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment
    Authors: B Guo, X Huang, S Cooper, HE Broxmeyer
    Nat. Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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