Recombinant Human Slit2 (aa1122-1529) Protein, CF Summary
Thr1122-Ser1529, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human Slit2 (Catalog # 9379-SL) Induces Cortical Neurite Outgrowth. A) Untreated E16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 625 ng/mL of Recombinant Human Slit2.
Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is widely expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including seven EGF-like domains, twenty Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and four N-terminal and four C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature human Slit2 shares 96% amino acid sequence identity with the mouse and rat orthologs. Slit2 has been shown to have various important functions in the nervous system. Slit2 induces growth cone collapse, inhibits oligodendrocyte precursor cell migration, and promotes axon elongation, branch formation, and fasciculation (5-9). Slit2 C-terminal fragment can mediate axon guidance through binding to Plexin A1 receptor (10). Slit2-C has also been shown to bind to Glypican-1 and promote motor axon migration (5). Outside the nervous system, C-terminal fragment of Slit2 activates a thermogenic PKA pathway in adipocytes and improves glucose homeostasis (11).
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- Liu, X. et al. (2012) J. Biol. Chem. 287:17503.
- Jaworski, A. and Tessier-Lavigne, M. (2012) Nat. Neurosci. 15:367.
- Delloye-Bourgeois, C. et.al. (2014) Nat. Neurosci. 18(1):36.
- Svensson, K.J. et al. (2016) Cell Metabolism. 23:1.
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