Recombinant Human SOCS-3 His-tag Protein, CF

R&D Systems | Catalog # 11817-S3

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Key Product Details

Source

E. coli

Accession Number

O14543.1

Applications

Bioactivity
View all SOCS-3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human SOCS-3 protein
Val2-Leu255, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val 2

Predicted Molecular Mass

25.5 kDa

SDS-PAGE

26-31 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human SOCS‑3 His-tag binds to Human/Mouse SOCS‑3 Antibody (Catalog # MAB5696) with an ED50 of 0.150-1.50 ng/mL.

Scientific Data Images for Recombinant Human SOCS-3 His-tag Protein, CF

Recombinant Human SOCS‑3 His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human SOCS‑3 His-tag Protein (Catalog # 11817-S3) binds to Human/Mouse SOCS-3 Antibody (MAB5696) with an ED50 of 0.150-1.50 ng/mL.

Recombinant Human SOCS‑3 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human SOCS‑3 His-tag Protein (Catalog # 11817-S3) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 26-31 kDa.

Formulation, Preparation, and Storage

11817-S3
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP and Glycerol with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: SOCS-3

Suppressor of Cytokine Signaling 3 (SOCS-3) is a key intracellular negative‑feedback regulator of cytokine signaling, functioning primarily within the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway. SOCS-3 is rapidly induced following cytokine stimulation and acts to constrain the intensity and duration of signaling downstream of multiple inflammatory mediators, particularly those utilizing gp130‑associated receptors, including interleukin‑6 (IL‑6) family cytokines (1, 2). Structurally, SOCS-3 contains an SH2 domain, a C‑terminal SOCS box, and an N‑terminal kinase inhibitory region (KIR). Mechanistic studies have demonstrated that SOCS-3 directly binds to JAK1, JAK2, and TYK2 while simultaneously engaging specific cytokine receptor phosphotyrosine motifs, conferring receptor‑restricted inhibition of JAK catalytic activity. This dual binding explains the selectivity of SOCS-3 for a subset of cytokine receptors, distinguishing it functionally from SOCS-1 (1, 3). SOCS-3 plays a critical role in immune homeostasis and inflammation, where it limits STAT-3‑dependent transcriptional programs and prevents pathological cytokine amplification. Genetic and conditional deletion studies have shown that SOCS-3 is essential for regulating inflammatory responses in myeloid and epithelial compartments, as well as for maintaining balanced signaling downstream of IL‑6, G‑CSF, leptin, and IL‑12 receptors (2, 4). In cancer and chronic inflammatory disease, SOCS-3 exhibits context‑dependent functions. Reduced SOCS-3 expression has been associated with sustained STAT3 activation, tumor‑promoting inflammation, and oncogenic signaling, while elevated SOCS-3 levels can contribute to immune evasion by dampening anti‑tumor cytokine responses. Consequently, SOCS-3 is increasingly recognized both as a disease biomarker and as a potential target for therapeutic modulation in cancer and inflammatory disorders (5, 6). Recombinant human SOCS-3 is therefore a valuable research tool for studying cytokine signal attenuation, JAK/STAT pathway specificity, gp130‑mediated signaling, inflammation, and tumor‑associated immune regulation, as well as for evaluating SOCS‑based therapeutic strategies.

References

  1. Babon, J. J. et al. (2012) Immunity 36:239.
  2. Mahony, R. et al. (2016) Cell. Mol. Life Sci. 73:3323.
  3. White, C. A. et al. (2013) JAK‑STAT 2:e25045.
  4. Carow, B. & Rottenberg, M. E. (2014) Front. Immunol. 5:58.
  5. Zhang, Y. et al. (2025) Biol. Proced. Online 27:36.
  6. Morelli, M. et al. (2024) Front. Immunol. 15:1393799.

Long Name

Suppressor Of Cytokine Signaling 3

Alternate Names

ATOD4, CIS3, Cish3, SOCS3, SSI-3

Entrez Gene IDs

9021 (Human); 12702 (Mouse); 89829 (Rat)

Gene Symbol

SOCS3

UniProt

O14543.1

Additional SOCS-3 Products

Product Documents for Recombinant Human SOCS-3 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human SOCS-3 His-tag Protein, CF

For research use only

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