Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 516919
View all SOCS-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOCS-3
Met1-Leu225
Accession # O14543

Specificity

Detects human or mouse SOCS-3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse SOCS‑3 Antibody

Detection of Human and Mouse SOCS-3 antibody by Western Blot.

Detection of Human and Mouse SOCS‑3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse SOCS-3 Monoclonal Antibody (Catalog # MAB5696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SOCS-3 by Western Blot

Detection of SOCS-3 by Western Blot

Aspirin modulated the JAK/p-STAT3 signaling pathway in atypical hyperplastic intestinal mucosal cells of UC mice (n = 4 for each group). (A) Western blotting images for JAK/p-STAT3 signaling pathway-associated molecules expression. (B) Statistical analysis and comparison for p-STAT3 expression. (C) Statistical analysis and comparison for STAT3 expression. (D) Statistical analysis and comparison for cyclin D1 expression. (E) Statistical analysis and comparison for SOCS3 expression. *P <.05 versus control group. #P <.05 versus UC model group. JAK, Janus kinase; UC, ulcerative colitis; p-STAT3, phosphorylated-STAT3; STAT3, signal transducer and activator of transcription 3; SOCS3, suppressor of cytokine signaling 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35946886), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SOCS-3 by Western Blot

Detection of SOCS-3 by Western Blot

Aspirin modulated the JAK/p-STAT3 signaling pathway in atypical hyperplastic intestinal mucosal cells of UC mice (n = 4 for each group). (A) Western blotting images for JAK/p-STAT3 signaling pathway-associated molecules expression. (B) Statistical analysis and comparison for p-STAT3 expression. (C) Statistical analysis and comparison for STAT3 expression. (D) Statistical analysis and comparison for cyclin D1 expression. (E) Statistical analysis and comparison for SOCS3 expression. *P <.05 versus control group. #P <.05 versus UC model group. JAK, Janus kinase; UC, ulcerative colitis; p-STAT3, phosphorylated-STAT3; STAT3, signal transducer and activator of transcription 3; SOCS3, suppressor of cytokine signaling 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35946886), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse SOCS‑3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB5696 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOCS-3

Suppressor of cytokine signaling 3 (SOCS-3), also known as CIS3, is a widely expressed 30 kDa protein that inhibits signal transduction induced by multiple cytokines including Erythropoietin, IL-6, LIF, Oncostatin M, and Leptin. It binds downstream signaling molecules such as JAK kinases and targets them for ubiquitin-mediated degradation, essential in a negative feedback control loop. Human SOCS-3 shares 96% aa sequence identity with mouse and rat SOCS-3.

Long Name

Suppressor Of Cytokine Signaling 3

Alternate Names

ATOD4, CIS3, Cish3, SOCS3, SSI-3

Entrez Gene IDs

9021 (Human); 12702 (Mouse); 89829 (Rat)

Gene Symbol

SOCS3

UniProt

O14543

Additional SOCS-3 Products

Product Documents for Human/Mouse SOCS‑3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SOCS‑3 Antibody

For research use only

Citations for Human/Mouse SOCS‑3 Antibody

Customer Reviews for Human/Mouse SOCS‑3 Antibody (1)

5 out of 5
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  • Human/Mouse SOCS-3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: C2C12 mouse myoblast cell line
    Species: Mouse
    Verified Customer | Posted 12/23/2021
    Human/Mouse SOCS‑3 Antibody MAB5696

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