Human/Mouse SOCS-3 Antibody Summary
Accession # O14543
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse SOCS‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse SOCS-3 Monoclonal Antibody (Catalog # MAB5696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Suppressor of cytokine signaling 3 (SOCS-3), also known as CIS3, is a widely expressed 30 kDa protein that inhibits signal transduction induced by multiple cytokines including Erythropoietin, IL-6, LIF, Oncostatin M, and Leptin. It binds downstream signaling molecules such as JAK kinases and targets them for ubiquitin-mediated degradation, essential in a negative feedback control loop. Human SOCS-3 shares 96% aa sequence identity with mouse and rat SOCS-3.
Citations for Human/Mouse SOCS-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
J. Immunol, 2017;0(0):.
Sample Types: Tissue Homogenates
Applications: Western Blot
Dysregulation of the suppressor of cytokine signalling 3-signal transducer and activator of transcription-3 pathway in the aetiopathogenesis of Sjogren's syndrome.
Authors: Vartoukian S, Tilakaratne W, Seoudi N, Bombardieri M, Bergmeier L, Tappuni A, Fortune F
Clin Exp Immunol, 2014;177(3):618-29.
Sample Types: Cell Lysates
Applications: Western Blot
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