Human/Mouse SOCS-3 Antibody

Catalog # Availability Size / Price Qty
MAB5696
MAB5696-SP

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Detection of Human and Mouse SOCS‑3 by Western Blot.
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Product Details
Citations (4)
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Reviews (1)

Human/Mouse SOCS-3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human or mouse SOCS-3 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 516919
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOCS-3
Met1-Leu225
Accession # O14543
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse SOCS‑3 by Western Blot. View Larger

Detection of Human and Mouse SOCS‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse SOCS-3 Monoclonal Antibody (Catalog # MAB5696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-3

Suppressor of cytokine signaling 3 (SOCS-3), also known as CIS3, is a widely expressed 30 kDa protein that inhibits signal transduction induced by multiple cytokines including Erythropoietin, IL-6, LIF, Oncostatin M, and Leptin. It binds downstream signaling molecules such as JAK kinases and targets them for ubiquitin-mediated degradation, essential in a negative feedback control loop. Human SOCS-3 shares 96% aa sequence identity with mouse and rat SOCS-3.

Long Name
Suppressor Of Cytokine Signaling 3
Entrez Gene IDs
9021 (Human); 12702 (Mouse); 89829 (Rat)
Alternate Names
ATOD4; CIS3; CIS3CIS-3; Cish3; cytokine-induced SH2 protein 3; Cytokine-inducible SH2 protein 3; SOCS3; SOCS-3; SOCS-3STAT-induced STAT inhibitor 3; SSI-3; SSI-3ATOD4; SSI3MGC71791; suppressor of cytokine signaling 3

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Citations for Human/Mouse SOCS-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation
    Authors: R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu
    International Journal of Molecular Sciences, 2021;22(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
    Authors: R Chakrabort, H Hu, C Darido, K Vickery, S Ranganatha
    International Journal of Molecular Sciences, 2021;22(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
    Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Dysregulation of the suppressor of cytokine signalling 3-signal transducer and activator of transcription-3 pathway in the aetiopathogenesis of Sjogren's syndrome.
    Authors: Vartoukian S, Tilakaratne W, Seoudi N, Bombardieri M, Bergmeier L, Tappuni A, Fortune F
    Clin Exp Immunol, 2014;177(3):618-29.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse SOCS-3 Antibody

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Human/Mouse SOCS-3 Antibody
By Anonymous on 12/23/2021
Application: WB Sample Tested: C2C12 mouse myoblast cell line Species: Mouse