Recombinant Human TfR Protein, CF

Catalog # Availability Size / Price Qty
2474-TR-050
Graph showing dose-dependent bioactivity of Human TfR protein
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Recombinant Human TfR Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When human Holo-Transferrin (Catalog # 2914-HT) is coated at 0.5 μg/mL (100 μL/well), the concentration of rhTfR that produces 50% of the optimal binding response is found to be approximately 3-12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human TfR (Transferrin R) protein
Cys89-Phe760, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
76 kDa
SDS-PAGE
76-85 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2474-TR

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Human TfR Protein View Larger

When human Holo-Transferrin (2914-HT) is coated at 0.5 μg/mL (100 μL/well), the concentration of recombinant human TfR (2474-TR) that produces 50% of the optimal binding response is found to be approximately 3-12 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TfR (Transferrin R)

The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1 - 4). As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome (2, 5). The human TfR cDNA encodes 760 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 672 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site (5). Human TfR ECD shares 75 - 80% aa identity with mouse, rat, feline, canine, equine, porcine and bovine TfR. A 679 aa alternately spliced form begins at aa 82 and is presumably secreted, while in an 804 aa form, 44 aa are inserted at aa 518 within the peptidase region (6). Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR (3). sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency (3). Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production (3). Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue (2, 4). Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis (2, 4). The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling (2, 5). TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses (2, 4, 7).

References
  1. Schneider, C. et al. (1984) Nature 311:675.
  2. Daniels, T.R. et al. (2006) Clin. Immunol. 121:144.
  3. Skikne, B.S. (2008) Am. J. Hematol. 83:872.
  4. Macedo, M.F. and M. deSousa (2008) Inflamm. Allergy Drug Targets 7:41.
  5. Aisen, P. (2004) Int. J. Biochem. Cell Biol. 36:2137.
  6. Entrez protein Accession # EAW53671, EAW53672.
  7. Radoshitzky, S.R. et al. (2007) Nature 446:92.
Long Name
Transferrin Receptor
Entrez Gene IDs
7037 (Human); 22042 (Mouse); 64678 (Rat)
Alternate Names
CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR

Citations for Recombinant Human TfR Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Generation of an iPSC line (SDQLCHi015-A) from peripheral blood mononuclear cells of a patient with mental retardation type 15 carrying c.1007_1011del, p.(Ile336fs) in CUL4B gene
    Authors: J Guan, X Liu, H Zhang, Y Lv, X Wang, X Yang, Y Ma, Q Liu, Y Liu, W Sun
    Stem Cell Res, 2019;41(0):101628.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. The impact of maternal obesity on iron status, placental transferrin receptor expression and hepcidin expression in human pregnancy.
    Authors: Garcia-Valdes L, Campoy C, Hayes H, Florido J, Rusanova I, Miranda M, McArdle H
    Int J Obes (Lond), 2015;39(4):571-8.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Standard)
  3. A human blood-brain barrier transcytosis assay reveals antibody transcytosis influenced by pH-dependent receptor binding.
    Authors: Sade H, Baumgartner C, Hugenmatter A, Moessner E, Freskgard P, Niewoehner J
    PLoS ONE, 2014;9(4):e96340.
    Species: Human
    Sample Types: Antibody
    Applications: Enzyme Assay

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Reviews for Recombinant Human TfR Protein, CF

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Recombinant Human TfR Protein, CF
By Anonymous on 10/11/2020
Application: Enzymatic activity in vitro

Recombinant Human TfR Protein, CF
By Anonymous on 01/27/2016
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