Human Holo-Transferrin Protein, CF

Catalog # Availability Size / Price Qty
2914-HT-001G
2914-HT-100MG
Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (2)

Human Holo-Transferrin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. Taub, M. et al. (1979) PNAS 76:3338. The ED50 for this effect is 0.075-0.375 μg/mL.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Human plasma-derived Holo-Transferrin protein

The human plasma used for the isolation of this product were certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

SDS-PAGE
76-81 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2914-HT

Formulation Lyophilized from a 0.2 μm filtered solution in NH4HCO3.
Reconstitution Reconstitute at 20 mg/mL in sterile, deionized water.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Holo-Transferrin

Human Transferrin (Tf) is a single chain, 80 kDa member of the anion-binding superfamily of proteins (1 - 5). It is a bilobed molecule that is the product of an ancient gene duplication event (1, 6). Transferrin is synthesized as a 698 amino acid (aa) precursor that is divided into a 19 aa signal sequence plus a 679 aa mature segment that contains 19 intrachain disulfide bonds. The crystal structure of Tf reveals a protein with two flanking 340 aa globular domains. Each are composed of a beta -sheet surrounded by series of alpha -helices (1, 7). The N- and C-terminal flanking regions (or domains) will bind ferric iron through the interaction of an obligate anion (usually bicarbonate) and four amino acids (His, Asp, and two Tyr) (7, 8). Apotransferrin (or iron‑free) will initially bind one atom of iron at the C-terminus, and this is followed by subsequent iron binding by the N‑terminus to form holotransferrin (diferric Tf) (8, 9). Through its C-terminal iron‑binding domain, holotransferrin will interact with the type I Tf receptor (TfR) on the surface of cells where it is internalized into acidified endosomes. Iron dissociates from the Tf molecule within these endosomes, and is transported into the cytosol as ferrous iron. At physiological pH, iron‑free apotransferrin is not bound by TfR. But at acidic pH, such as exists in the endosome, apotransferrin has considerable affinity for TfR. Thus, it remains bound to TfR and is recycled back to the cell surface where a neutral pH environment dissociates ligand from receptor. Each Tf molecule recycles 100 - 150 times during its lifetime (8 - 11). In addition to TfR, transferrin is reported to bind to cubulin, IGFBP3, microbial iron‑binding proteins and liver-specific TfR2 (7, 12, 13, 14). Transferrin is variably glycosylated and the degree of sialylation is suggestive of certain clinical conditions (15). Finally, Tf is highly allelic and the gene codominant, with many single aa changes noted. Three general forms are known, based on standard electrophoretic mobility. Fast Tf is known as transferrin B, slow transferrin is transferrin D, and the middle migrating transferrin is type/variant C, thre most common (16, 17). Mature human TF is 73% aa identical to both mouse and rat Tf, and 68% and 71% aa identical to bovine and equine Tf, respectively.

References
  1. Brus, C.M. et al. (2001) Nat. Struct. Biol. 4:919.
  2. Schaeffer, E. et al. (1987) Gene 56:109.
  3. MacGillivray, R.T.A. et al. (1983) J. Biol. Chem. 258:3543.
  4. Yang, F. et al. (1984) Proc. Natl. Acad. Sci. USA 81:2752.
  5. Uzan, G. et al. (1984) Biochem. Biophys. Res. Commun. 119:273.
  6. Zak, O. et al. (2002) Biochemistry 41:7416.
  7. Gomme, P.T. and K. B. McCann (2005) Drug Discov. Today 10:267.
  8. Liu, R. et al. (2003) Biochemistry 42:12447.
  9. Pakdaman, R. et al. (1999) J. Mol. Biol. 293:1273.
  10. Hemadi, M. et al. (2004) Biochemistry 43:1736.
  11. Aisen, P. et al. (2001) Int. J. Biochem. Cell Biol. 33:940.
  12. Kozyraki, R. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12941.
  13. Boulton, I.C. et al. (1998) Biochem. J. 334:269.
  14. Robb, A. and M. Wessling-Resnick (2004) Blood 104:4294.
  15. Landberg, E. et al. (1995) Biochem. Biophys. Res. Commun. 210:267.
  16. Gorg, A. et al. (1983) Hum. Genet. 64:222.
  17. Bean, P. and J.B. Peter (1994) Clin. Chem. 40:2078.
Entrez Gene IDs
7018 (Human)
Alternate Names
HoloTransferrin; Holo-Transferrin

Citations for Human Holo-Transferrin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene
    Authors: H Zhang, C Liu, Y Ma, L Lin, Y Lv, M Gao, Z Gai, Y Liu
    Stem Cell Research, 2021;53(0):102331.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene
    Authors: Z Li, X Zhang, X Li, Y Yang, H Xin, X Yang, N Liu, Z Gai, Y Liu
    Stem Cell Research, 2021;53(0):102313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Expression of South East Asian Ovalocytic Band 3 Disrupts Erythroblast Cytokinesis and Reticulocyte Maturation
    Authors: JF Flatt, CJ Stevens-He, NM Cogan, DJ Eggleston, NM Haines, KJ Heesom, V Picard, C Thomas, LJ Bruce
    Front Physiol, 2020;11(0):357.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Generation of patient-specific pluripotent induced stem cell line SDUBMSI002-A from a patient with X-linked mental retardation syndrome
    Authors: X Liu, X Yang, Y Li, X Wang, J Ma, W Jiang, Y Liu, W Sun, Y Gong
    Stem Cell Res, 2020;43(0):101724.
    Species: Human
    Sample Types: Whole Cells
  5. Generation of a Human iPSC line (SDQLCHi021-A) from a patient with methylmalonic acidemia cblC type carrying compound heterozygous mutations in MMAHC gene
    Authors: J Guan, Z Li, H Zhang, X Yang, Y Ma, Y Li, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2020;43(0):101709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Generation of a human induced pluripotent stem cell line (SDUBMSi001-A) from a hereditary spastic paraplegia patient carrying kif1a c.773C>T missense mutation
    Authors: W Xiaojing, M Yanyan, D Ruonan, L Xiaolin, Z Haiyan, M Jian, L Yi, S Wenjie, L Qiji
    Stem Cell Res, 2020;43(0):101727.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. An integration-free iPSC line (SDQLCHi017-A) derived from a patient with nemaline myopathy-2 disease carrying compound heterozygote mutations in NEB gene
    Authors: Y Ma, H Zhang, X Li, X Yang, Y Li, J Guan, Y Lv, Z Gai, Y Liu
    Stem Cell Res, 2020;43(0):101729.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Stage Specific Expression Pattern of Alpha-Hemoglobin-Stabilizing-Protein (AHSP) Portrayed in Erythroblast Chronology
    Authors: J Walczak, MD Camargo Jo, K Muthumalai
    Methods Protoc, 2020;3(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. An integration-free iPSC line SDQLCHi025-A from a girl with multiminicore disease carrying compound heterozygote mutations in RYR1 gene
    Authors: H Zhang, Y Ma, Y Lv, Y Wan, Q Zhao, Z Gai, Y Liu
    Stem Cell Res, 2020;45(0):101775.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Cystatin B is essential for proliferation and interneuron migration in individuals with EPM1 epilepsy
    Authors: F Di Matteo, F Pipicelli, C Kyrousi, I Tovecci, E Penna, M Crispino, A Chambery, R Russo, AC Ayo-Martin, M Giordano, A Hoffmann, E Ciusani, L Canafoglia, M Götz, R Di Giaimo, S Cappello
    EMBO Mol Med, 2020;0(0):e11419.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Using inducible lentiviral vectors to generate induced pluripotent stem cell line ZOCi001-A from peripheral blood cells of a patient with CRB1-/- retinitis pigmentosa
    Authors: X Tang, X Liu, Z Chen, L Luo, X Liu, J Deng, DW Li, Y Liu
    Stem Cell Res, 2020;45(0):101817.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  12. Generation of an induced pluripotent stem cell line (SDQLCHi009-A) from a patient with 47,XXY and ornithine transcarbamylase deficiency carrying a hemizygote mutation in OTC
    Authors: X Yang, B Yan, H Zhang, Y Ma, Q Zhou, Y Li, J Guan, D Wang, Y Liu, Z Gai
    Stem Cell Res, 2020;43(0):101704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene
    Authors: H Zhang, Y Ma, S Yu, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2019;41(0):101611.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  14. An integration-free iPSC line (SDQLCHi013-A) derived from a patient with maple syrup urine disease carrying compound heterozygote mutations in BCKDHA gene
    Authors: H Zhang, Y Ma, X Li, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2019;41(0):101585.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  15. Establishment of a human induced pluripotent stem cell line (SDQLCHi004-A) from a patient with nemaline myopathy-4 disease carrying heterozygous mutation in TPM2 gene
    Authors: Y Ma, H Zhang, X Yang, Y Li, J Guan, Y Lv, H Li, Y Liu, Z Gai
    Stem Cell Res, 2019;40(0):101559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
    Authors: Y Ma, H Zhang, S Zhang, R Dong, X Yang, Y Li, J Guan, Z Gai, Y Liu
    Stem Cell Res, 2019;41(0):101577.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  17. An induced pluripotent stem cell line (SDQLCHi006-A) derived from a patient with maple syrup urine disease type Ib carrying compound heterozygous mutations of p.R168C and p.T322I in BCKDHB gene
    Authors: Y Li, H Zhang, B Yan, Y Ma, X Yang, J Guan, Y Lv, M Gao, J Ma, Z Gai, Y Liu
    Stem Cell Res, 2019;40(0):101579.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  18. Highly Efficient and Marker-free Genome Editing of Human Pluripotent Stem Cells by CRISPR-Cas9 RNP and AAV6 Donor-Mediated Homologous Recombination
    Authors: RM Martin, K Ikeda, MK Cromer, N Uchida, T Nishimura, R Romano, AJ Tong, VT Lemgart, J Camarena, M Pavel-Dinu, C Sindhu, V Wiebking, S Vaidyanath, DP Dever, RO Bak, A Laustsen, BJ Lesch, MR Jakobsen, V Sebastiano, H Nakauchi, MH Porteus
    Cell Stem Cell, 2019;24(5):821-828.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  19. Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease
    Authors: K Denis, M Le Bris, L Le Guennec, JP Barnier, C Faure, A Gouge, H Bouzinba-S, A Jamet, D Euphrasie, B Durel, N Barois, P Pelissier, PC Morand, M Coureuil, F Lafont, O Join-Lambe, X Nassif, S Bourdoulou
    Nat Microbiol, 2019;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. Optimization of an erythroid culture system to reduce the cost of in vitro production of red blood cells
    Authors: S Poldee, C Metheetrai, S Nugoolsuks, J Frayne, K Trakarnsan
    MethodsX, 2018;5(0):1626-1632.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage
    Authors: BM Hopkinson, C Desler, M Kalisz, PS Vestentoft, L Juel Rasmu, HC Bisgaard
    Oxid Med Cell Longev, 2017;2017(0):5080128.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Experimental evidences of human coronary microvasculature and myocardial tissue bacterial colonization during meningococcemia
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation.
    Authors: Huang X, Wang Y, Yan W, Smith C, Ye Z, Wang J, Gao Y, Mendelsohn L, Cheng L
    Stem Cells, 2015;33(5):1470-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Systemic combinatorial peptide selection yields a non-canonical iron-mimicry mechanism for targeting tumors in a mouse model of human glioblastoma.
    Authors: Staquicini FI, Ozawa MG, Moya CA, Driessen WH, Barbu EM, Nishimori H, Soghomonyan S, Flores LG, Liang X, Paolillo V, Alauddin MM, Basilion JP, Furnari FB, Bogler O, Lang FF, Aldape KD, Fuller GN, Höök M, Gelovani JG, Sidman RL, Cavenee WK, Pasqualini R, Arap W
    J. Clin. Invest., 2011;121(1):161-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Human Holo-Transferrin Protein, CF

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Human Holo-Transferrin Protein, CF
By Anonymous on 12/15/2016
Application: Stem/Immune cell maintenance or differentiation

We use human Transferrin to supplement media for murine embryonic stem cells (ESC) differentiation in embryoid bodies (EBs) and further to hemogenic endothelium from which hematopoietic stem and progenitor cells emerge.


Human Holo-Transferrin Protein, CF
By Anonymous on 01/28/2016
Application: