Recombinant Human TNF RI/TNFRSF1A Fc Chimera Protein

Carrier Free

Catalog # Availability Size / Price Qty
372-RI-050/CF

With Carrier

Catalog # Availability Size / Price Qty
372-RI-050
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Citations (11)
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Recombinant Human TNF RI/TNFRSF1A Fc Chimera Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.4‑2 ng/mL in the presence of 0.25 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA).
Source
Mouse myeloma cell line, NS0-derived human TNF RI/TNFRSF1A protein
Human TNF RI
(Leu30-Thr211) &
(Asp41-Thr211)
Accession # P19438
IEGRMD Human IgG1
(Pro100-Lys330)
6 His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu30 & Asp41
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48 kDa (monomer)
SDS-PAGE
60-66 kDa, reducing conditions

Product Datasheets

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372-RI (with carrier)

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372-RI/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

372-RI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

372-RI/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence and 190 aa ECD with a PLAD (pre-ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of human TNF RI shows 70%, 69%, 80%, 80%, and 73% aa identity with mouse, rat, canine, feline and porcine TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro‑inflammatory stimuli (7). TNF-alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8, 9, 16). Release of the 28-34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10-12). Full-length TNF RI may also be released in exosome-like vesicles (13). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR-associated periodic syndrome) (7).

References
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Chan, F.K. et al. (2000) Science 288:2351.
  4. Schall, T.J. et al. (1990) Cell 61:361.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  6. Banner, D.W et al. (1993) Cell 73: 431.
  7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
  8. Legler, D.F. et al. (2003) Immunity 18:655.
  9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  11. Jin, L. et al. (2000) J. Immunol. 165:5153.
  12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  15. McDermott, M.F. et al. (1999) Cell 97:133.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

Citations for Recombinant Human TNF RI/TNFRSF1A Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Bispecific soluble cytokine receptor-nanobody fusions inhibit Interleukin (IL-)6 trans-signaling and IL-12/23 or tumor necrosis factor (TNF) signaling
    Authors: Gesiorowski, A;Ettich, J;Werner, J;Wittich, C;Pieper, S;Padrini, G;Behnke, K;Floss, DM;Lang, PA;Moll, JM;Scheller, J;
    The Journal of biological chemistry
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF
    Authors: DJ Lightwood, RJ Munro, J Porter, D McMillan, B Carrington, A Turner, A Scott-Tuck, ES Hickford, A Schmidt, D Fox, A Maloney, T Ceska, T Bourne, J O'Connell, ADG Lawson
    Nature Communications, 2021-01-25;12(1):583.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  3. Defective Regulation of Membrane TNF&alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells
    Authors: T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh
    Int J Mol Sci, 2020-04-21;21(8):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  4. A trimeric structural fusion of an antagonistic tumor necrosis factor-? mutant enhances molecular stability and enables facile modification
    Authors: M Inoue, D Ando, H Kamada, S Taki, M Niiyama, Y Mukai, T Tadokoro, K Maenaka, T Nakayama, Y Kado, T Inoue, Y Tsutsumi, SI Tsunoda
    J. Biol. Chem, 2017-02-24;0(0):.
    Applications: Bioassay
  5. The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells.
    Authors: Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T
    J Biol Chem, 2014-02-18;289(14):10029-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Cell Culture
  6. Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
    Authors: Moh M, Lorenzini P, Gullo C, Schwarz H
    FASEB J, 2013-04-25;27(8):2957-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection.
    Authors: Cosgrove C, Ussher J, Rauch A, Gartner K, Kurioka A, Huhn M, Adelmann K, Kang Y, Fergusson J, Simmonds P, Goulder P, Hansen T, Fox J, Gunthard H, Khanna N, Powrie F, Steel A, Gazzard B, Phillips R, Frater J, Uhlig H, Klenerman P
    Blood, 2012-12-18;121(6):951-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008-06-09;76(8):3530-8.
    Species: Bacteria
    Sample Types: Trypanosoma
    Applications: PcIP
  9. Role of interleukin-1beta and tumor necrosis factor-alpha-dependent expression of cyclooxygenase-2 mRNA in thermal hyperalgesia induced by chronic inflammation in mice.
    Authors: Narita M, Shimamura M, Imai S, Kubota C, Yajima Y, Takagi T, Shiokawa M, Inoue T, Suzuki M, Suzuki T
    Neuroscience, 2007-11-12;152(2):477-86.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression.
    Authors: Hu P, Han Z, Couvillon AD, Kaufman RJ, Exton JH
    Mol. Cell. Biol., 2006-04-01;26(8):3071-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Inflammatory cytokine regulation of TRAIL-mediated apoptosis in thyroid epithelial cells.
    Authors: Bretz JD, Mezosi E, Giordano TJ, Gauger PG, Thompson NW, Baker JR
    Cell Death Differ., 2002-03-01;9(3):274-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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