Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF Summary
|Human TRAIL R2/TNFRSF10B|
Accession # AAH01281.1
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 10140-T2) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 0.75-6 ng/mL.
Background: TRAIL R2/TNFRSF10B
Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand) (1). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. There are no known rat or mouse homologs to TRAILR2. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis (1-2). The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis (1-2). Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported (3).
- Chaudhary, P.M. et al. (1997) Immunity 7:821.
- Walczak, H. et al. (1997) EMBO J. 16:5386.
- Golstein, P. (1997) Curr. Biol. 7:R750.
Citations for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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DR5-Selective TRAIL Variant DR5-B Functionalized with Tumor-Penetrating iRGD Peptide for Enhanced Antitumor Activity against Glioblastoma
Authors: AV Yagolovich, AA Isakova, AA Artykov, YV Vorontsova, DV Mazur, NV Antipova, MS Pavlyukov, MI Shakhparon, AM Gileva, EA Markvichev, EA Plotnikova, AA Pankratov, MP Kirpichnik, ME Gasparian, DA Dolgikh
International Journal of Molecular Sciences, 2022;23(20):.
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Dual role of ERK2/NF-kappaB signaling in TRAIL sensitivity
Authors: MW Lee, DS Kim, JE Eom, JW Lee, KW Sung, HH Koo, YB Hong, KH Yoo
American journal of cancer research, 2022;12(7):3373-3389.
Sample Types: Whole Cell
Applications: Cell Culture
TRAILshort Protects against CD4 T Cell Death during Acute HIV Infection
Authors: S Natesampil, AC Paim, NW Cummins, AP Chandrasek, GD Bren, SR Lewin, HP Kiem, AD Badley
J. Immunol., 2019;0(0):.
Sample Types: Cell Lysates
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