The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.
Recombinant Human TrkB Fc Chimera Protein, CF
R&D Systems | Catalog # 688-TK
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Key Product Details
- R&D Systems NS0-derived Recombinant Human TrkB Fc Chimera Protein (688-TK)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TrkB protein
| HumanTrkB (Cys32-His430) Accession # Q16620.1 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Cys32
Predicted Molecular Mass
71.7 kDa (monomer)
SDS-PAGE
120 kDa-130 kDa, reducing conditions
Activity
Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB.
The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.
The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.
Reviewed Applications
Read 3 reviews rated 4.7 using 688-TK in the following applications:
Scientific Data Images for Recombinant Human TrkB Fc Chimera Protein, CF
Recombinant Human TrkB Fc Chimera Protein Bioactivity
Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) inhibits BDNF-induced cell proliferation of the BaF‑TrkB‑BD mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.1-0.4 μg/mL in the presence of 16 ng/mL of recombinant human BDNF.
Recombinant Human TrkB Fc Chimera Protein SDS-PAGE
1 μg/lane of Recombinant Human TrkB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 120 kDa and 200 kDa, respectively.Formulation, Preparation, and Storage
688-TK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: TrkB
References
- Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
- Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
- Soppet, D. et al. (1991) Cell 65:895.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Alternate Names
NTRK2
Gene Symbol
NTRK2
UniProt
Additional TrkB Products
Product Documents for Recombinant Human TrkB Fc Chimera Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human TrkB Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human TrkB Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human TrkB Fc Chimera Protein, CF (3)
4.7 out of 5
3 Customer Ratings
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 09/07/2018Rat cortical neurons treated with 4ug/ml TrkB at 37C for 30min then with 5ng/ml Bdnf for 1h, and harvested for RT-PCR with primers specific for cFos.
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Application: In vivo studyVerified Customer | Posted 07/18/2016
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Application: Apoptosis assayVerified Customer | Posted 05/18/2016
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
