Recombinant Human TrkB Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
688-TK-100
Recombinant Human TrkB Fc Chimera Protein, CF Bioactivity
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Product Details
Citations (42)
FAQs
Supplemental Products
Reviews (3)

Recombinant Human TrkB Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.
Source
Mouse myeloma cell line, NS0-derived human TrkB protein
HumanTrkB
(Cys32-His430)
Accession # Q16620.1
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Cys32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
71.7 kDa (monomer)
SDS-PAGE
120 kDa-130 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

688-TK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) inhibits BDNF-induced cell proliferation of the BaF‑TrkB‑BD mouse pro-B cell line transfected with TrkB. The ED50for this effect is 0.1-0.4 μg/mL in the presence of 16 ng/mL of recombinant human BDNF.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human TrkB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 120 kDa and 200 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

References
  1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  3. Soppet, D. et al. (1991) Cell 65:895.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs
4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B

Citations for Recombinant Human TrkB Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Exercise training modulates glutamic acid decarboxylase-65/67 expression through TrkB signaling to ameliorate neuropathic pain in rats with spinal cord injury
    Authors: X Li, Q Wang, J Ding, S Wang, C Dong, Q Wu
    Mol Pain, 2020;16(0):1744806920924.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. Loss of TrkB Signaling Due to Status Epilepticus Induces a proBDNF-Dependent Cell Death
    Authors: LE Montroull, V Danelon, AB Cragnolini, DH Mascó
    Front Cell Neurosci, 2019;13(0):4.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Antidepressant-like effect of losartan involves TRKB transactivation from angiotensin receptor type 2 (AGTR2) and recruitment of FYN
    Authors: CRAF Diniz, PC Casarotto, SM Fred, C Biojone, E Castrén, SRL Joca
    Neuropharmacology, 2018;135(0):163-171.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Modulation of AMPA receptor surface diffusion restores hippocampal plasticity and memory in Huntington's disease models
    Authors: H Zhang, C Zhang, J Vincent, D Zala, C Benstaali, M Sainlos, D Grillo-Bos, S Daburon, F Coussen, Y Cho, DJ David, F Saudou, Y Humeau, D Choquet
    Nat Commun, 2018;9(1):4272.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Nerve Growth Factor Is Responsible for Exercise-Induced Recovery of Septohippocampal Cholinergic Structure and Function
    Authors: JM Hall, F Gomez-Pini, LM Savage
    Front Neurosci, 2018;12(0):773.
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo
  6. Human menstrual blood-derived stem cells promote functional recovery in a rat spinal cord hemisection model
    Authors: Q Wu, Q Wang, Z Li, X Li, J Zang, Z Wang, C Xu, Y Gong, J Cheng, H Li, G Shen, C Dong
    Cell Death Dis, 2018;9(9):882.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. Blocking of BDNF-TrkB signaling inhibits the promotion effect of neurological function recovery after treadmill training in rats with spinal cord injury
    Authors: X Li, Q Wu, C Xie, C Wang, Q Wang, C Dong, L Fang, J Ding, T Wang
    Spinal Cord, 2018;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. BDNF/NT3 Regulate AIS Location and Affect Neuronal Excitability in Cultured Hippocampal Neurons
    Authors: Y Guo, ZJ Su, YK Chen, Z Chai
    J. Neurochem., 2017;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  9. Dynamic Arc SUMOylation and Selective Interaction with F-Actin-Binding Protein Drebrin A in LTP Consolidation In Vivo
    Authors: RR Nair, S Patil, A Tiron, T Kanhema, D Panja, L Schiro, K Parobczak, G Wilczynski, CR Bramham
    Front Synaptic Neurosci, 2017;9(0):8.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  10. TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus
    Authors: JI Hurtado-Za, B Ramachandr, S Ahmed, R Halder, C Bolleyer, A Awasthi, MA Stahlberg, RJ Wagener, K Anderson, RM Drenan, HA Lester, JM Miwa, JF Staiger, A Fischer, C Dean
    Nat Commun, 2017;8(0):15878.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons
    Authors: M Sharma, T Dierkes, S Sajikumar
    Aging Cell, 2017;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  12. Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth
    Authors: A Suzuki, D Matsuura, H Kanatani, S Yano, M Tsunakawa, S Matsuyama, H Shigemori
    Biol. Pharm. Bull., 2017;40(10):1784-1788.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  13. Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations
    Authors: JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  14. Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
    Authors: LF Martins, RO Costa, JR Pedro, P Aguiar, SC Serra, FG Teixeira, N Sousa, AJ Salgado, RD Almeida
    Sci Rep, 2017;7(1):4153.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. BK channels in microglia are required for morphine-induced hyperalgesia
    Authors: Yoshinori Hayashi
    Nat Commun, 2016;7(0):11697.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Complete spinal cord injury (SCI) transforms how brain derived neurotrophic factor (BDNF) affects nociceptive sensitization
    Authors: James W Grau
    Exp. Neurol., 2016;288(0):38-50.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  17. Potentiation of Synaptic GluN2B NMDAR Currents by Fyn Kinase Is Gated through BDNF-Mediated Disinhibition in Spinal Pain Processing
    Cell Rep, 2016;17(10):2753-2765.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Different patterns of electrical activity lead to long-term potentiation by activating different intracellular pathways.
    Authors: Zhu G, Liu Y, Wang Y, Bi X, Baudry M
    J Neurosci, 2015;35(2):621-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  19. Different immune cells mediate mechanical pain hypersensitivity in male and female mice.
    Authors: Sorge R, Mapplebeck J, Rosen S, Beggs S, Taves S, Alexander J, Martin L, Austin J, Sotocinal S, Chen D, Yang M, Shi X, Huang H, Pillon N, Bilan P, Tu Y, Klip A, Ji R, Zhang J, Salter M, Mogil J
    Nat Neurosci, 2015;18(8):1081-3.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
    Authors: Obis T, Besalduch N, Hurtado E, Nadal L, Santafe M, Garcia N, Tomas M, Priego M, Lanuza M, Tomas J
    Mol Brain, 2015;8(0):8.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  21. PAR2-mediated upregulation of BDNF contributes to central sensitization in bone cancer pain.
    Authors: Bao Y, Hou W, Liu R, Gao Y, Kong X, Yang L, Shi Z, Li W, Zheng H, Jiang S, Li C, Qin Y, Hua B
    Mol Pain, 2014;10(0):28.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  22. A positive autoregulatory BDNF feedback loop via C/EBPbeta mediates hippocampal memory consolidation.
    Authors: Bambah-Mukku D, Travaglia A, Chen D, Pollonini G, Alberini C
    J Neurosci, 2014;34(37):12547-59.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  23. Dynorphin up-regulation in the dentate granule cell mossy fiber pathway following chronic inhibition of GluN2B-containing NMDAR is associated with increased CREB (Ser 133) phosphorylation, but is independent of BDNF/TrkB signaling pathways.
    Authors: Rittase, W Bradle, Dong, Yu, Barksdale, DaRel, Galdzicki, Zygmunt, Bausch, Suzanne
    Mol Cell Neurosci, 2014;60(0):63-71.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  24. Heterogeneous intracellular trafficking dynamics of brain-derived neurotrophic factor complexes in the neuronal soma revealed by single quantum dot tracking.
    Authors: Vermehren-Schmaedick, Anke, Krueger, Wesley, Jacob, Thomas, Ramunno-Johnson, Damien, Balkowiec, Agnieszk, Lidke, Keith A, Vu, Tania Q
    PLoS ONE, 2014;9(4):e95113.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  25. BDNF regulates atypical PKC at spinal synapses to initiate and maintain a centralized chronic pain state.
    Authors: Melemedjian O, Tillu D, Asiedu M, Mandell E, Moy J, Blute V, Taylor C, Ghosh S, Price T
    Mol Pain, 2013;9(0):12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. Glucocorticoid receptors recruit the CaMKIIalpha-BDNF-CREB pathways to mediate memory consolidation.
    Authors: Chen D, Bambah-Mukku D, Pollonini G, Alberini C
    Nat Neurosci, 2012;15(12):1707-14.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  27. Inhibition of uterine sarcoma cell growth through suppression of endogenous tyrosine kinase B signaling.
    Authors: Makino, Kenichi, Kawamura, Kazuhiro, Sato, Wataru, Kawamura, Nanami, Fujimoto, Toshio, Terada, Yukihiro
    PLoS ONE, 2012;7(7):e41049.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Brain-derived neurotrophic factor ameliorates brain stem cardiovascular dysregulation during experimental temporal lobe status epilepticus.
    Authors: Tsai CY, Chan JY, Hsu KS
    PLoS ONE, 2012;7(3):e33527.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  29. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: N/A
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  30. Brain-derived neurotrophic factor modulates antiretroviral-induced mechanical allodynia in the mouse.
    Authors: Renn CL, Leitch CC, Lessans S, Rhee P, McGuire WC, Smith BA, Traub RJ, Dorsey SG
    J. Neurosci. Res., 2011;89(10):1551-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  31. Peptides derived from the solvent-exposed loops 3 and 4 of BDNF bind TrkB and p75(NTR) receptors and stimulate neurite outgrowth and survival.
    Authors: Fobian K, Owczarek S, Budtz C
    J. Neurosci. Res., 2010;88(6):1170-81.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  32. BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
    Int. J. Oncol., 2010;36(6):1469-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Early exercise in spinal cord injured rats induces allodynia through TrkB signaling.
    Authors: Endo T, Ajiki T, Inoue H, Kikuchi M, Yashiro T, Nakama S, Hoshino Y, Murakami T, Kobayashi E
    Biochem. Biophys. Res. Commun., 2009;381(3):339-44.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  34. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008;76(8):3530-8.
    Species: Bacteria
    Sample Types: Trypanosoma
    Applications: PcIP
  35. BDNF-exercise interactions in the recovery of symmetrical stepping after a cervical hemisection in rats.
    Authors: Ying Z, Roy RR, Zhong H, Zdunowski S, Edgerton VR, Gomez-Pinilla F
    Neuroscience, 2008;155(4):1070-8.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  36. Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity: a role for microglia.
    Authors: Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO
    Eur. J. Neurosci., 2008;27(8):2089-102.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Brain-derived neurotrophic factor functions as a metabotrophin to mediate the effects of exercise on cognition.
    Authors: Gomez-Pinilla F, Vaynman S, Ying Z
    Eur. J. Neurosci., 2008;28(11):2278-87.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  38. Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions.
    Authors: Fitzgerald M, Buckley A, Lukehurst SS, Dunlop SA, Beazley LD, Rodger J
    Biochem. Biophys. Res. Commun., 2008;374(4):625-30.
  39. BDNF and learning: Evidence that instrumental training promotes learning within the spinal cord by up-regulating BDNF expression.
    Authors: Gomez-Pinilla F, Huie JR, Ying Z, Ferguson AR, Crown ED, Baumbauer KM, Edgerton VR, Grau JW
    Neuroscience, 2007;148(4):893-906.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  40. Exercise normalizes levels of MAG and Nogo-A growth inhibitors after brain trauma.
    Authors: Chytrova G, Ying Z, Gomez-Pinilla F
    Eur. J. Neurosci., 2007;27(1):1-11.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  41. Exercise differentially regulates synaptic proteins associated to the function of BDNF.
    Authors: Vaynman SS, Ying Z, Yin D, Gomez-Pinilla F
    Brain Res., 2006;1070(1):124-30.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  42. Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
    Authors: Esper RM, Loeb JA
    J. Neurosci., 2004;24(27):6218-27.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human TrkB Fc Chimera Protein, CF

Average Rating: 4.7 (Based on 3 Reviews)

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Recombinant Human TrkB Fc Chimera Protein, CF
By Anonymous on 09/07/2018
Application: In vitro bioactivity in cell culture

Rat cortical neurons treated with 4ug/ml TrkB at 37C for 30min then
with 5ng/ml Bdnf for 1h, and harvested for RT-PCR with primers specific for cFos.


Recombinant Human TrkB Fc Chimera Protein, CF
By Anonymous on 07/18/2016
Application: In vivo study

Recombinant Human TrkB Fc Chimera Protein, CF
By Anonymous on 05/18/2016
Application: Apoptosis assay
Reason for Rating: it blocks the activation of TrkB via BDNF.