Recombinant Human TrkB Fc Chimera Protein, CF

(31 citations)
(2 Reviews)
  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.
  • Source
    Mouse myeloma cell line, NS0-derived
    HumanTrkB
    (Cys32-His430)
    Accession # Q16620.1
    DIEGRMD Human IgG1
    (Pro100-Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Cys32
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    71.7 kDa (monomer)
  • SDS-PAGE
    120 kDa-130 kDa, reducing conditions
688-TK
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) inhibits BDNF-induced cell proliferation of the BaF‑TrkB‑BD mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.1-0.4 μg/mL in the presence of 16 ng/mL of recombinant human BDNF.
1 μg/lane of Recombinant Human TrkB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 120 kDa and 200 kDa, respectively.
Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

  • References:
    1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
    2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
    3. Soppet, D. et al. (1991) Cell 65:895.
  • Long Name:
    Neurotrophic Tyrosine Kinase Receptor B
  • Entrez Gene IDs:
    4915 (Human); 18212 (Mouse); 25054 (Rat)
  • Alternate Names:
    BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons
    Authors: M Sharma, T Dierkes, S Sajikumar
    Aging Cell, 2017;0(0):.
    Species: N/A
    Sample Type: Proteins
    Application: Bioassay
  2. TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus
    Authors: JI Hurtado-Za, B Ramachandr, S Ahmed, R Halder, C Bolleyer, A Awasthi, MA Stahlberg, RJ Wagener, K Anderson, RM Drenan, HA Lester, JM Miwa, JF Staiger, A Fischer, C Dean
    Nat Commun, 2017;8(0):15878.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
    Authors: LF Martins, RO Costa, JR Pedro, P Aguiar, SC Serra, FG Teixeira, N Sousa, AJ Salgado, RD Almeida
    Sci Rep, 2017;7(1):4153.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations
    Authors: JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  5. Potentiation of Synaptic GluN2B NMDAR Currents by Fyn Kinase Is Gated through BDNF-Mediated Disinhibition in Spinal Pain Processing
    Cell Rep, 2016;17(10):2753-2765.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  6. BK channels in microglia are required for morphine-induced hyperalgesia
    Authors: Yoshinori Hayashi
    Nat Commun, 2016;7(0):11697.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  7. Different patterns of electrical activity lead to long-term potentiation by activating different intracellular pathways.
    Authors: Zhu G, Liu Y, Wang Y, Bi X, Baudry M
    J Neurosci, 2015;35(2):621-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  8. The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
    Authors: Obis T, Besalduch N, Hurtado E, Nadal L, Santafe M, Garcia N, Tomas M, Priego M, Lanuza M, Tomas J
    Mol Brain, 2015;8(0):8.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Bioassay
  9. Different immune cells mediate mechanical pain hypersensitivity in male and female mice.
    Authors: Sorge R, Mapplebeck J, Rosen S, Beggs S, Taves S, Alexander J, Martin L, Austin J, Sotocinal S, Chen D, Yang M, Shi X, Huang H, Pillon N, Bilan P, Tu Y, Klip A, Ji R, Zhang J, Salter M, Mogil J
    Nat Neurosci, 2015;18(8):1081-3.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  10. Dynorphin up-regulation in the dentate granule cell mossy fiber pathway following chronic inhibition of GluN2B-containing NMDAR is associated with increased CREB (Ser 133) phosphorylation, but is independent of BDNF/TrkB signaling pathways.
    Authors: Rittase, W Bradle, Dong, Yu, Barksdale, DaRel, Galdzicki, Zygmunt, Bausch, Suzanne
    Mol Cell Neurosci, 2014;60(0):63-71.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Bioassay
  11. Heterogeneous intracellular trafficking dynamics of brain-derived neurotrophic factor complexes in the neuronal soma revealed by single quantum dot tracking.
    Authors: Vermehren-Schmaedick, Anke, Krueger, Wesley, Jacob, Thomas, Ramunno-Johnson, Damien, Balkowiec, Agnieszk, Lidke, Keith A, Vu, Tania Q
    PLoS ONE, 2014;9(4):e95113.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  12. PAR2-mediated upregulation of BDNF contributes to central sensitization in bone cancer pain.
    Authors: Bao Y, Hou W, Liu R, Gao Y, Kong X, Yang L, Shi Z, Li W, Zheng H, Jiang S, Li C, Qin Y, Hua B
    Mol Pain, 2014;10(0):28.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  13. A positive autoregulatory BDNF feedback loop via C/EBPbeta mediates hippocampal memory consolidation.
    Authors: Bambah-Mukku D, Travaglia A, Chen D, Pollonini G, Alberini C
    J Neurosci, 2014;34(37):12547-59.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  14. BDNF regulates atypical PKC at spinal synapses to initiate and maintain a centralized chronic pain state.
    Authors: Melemedjian O, Tillu D, Asiedu M, Mandell E, Moy J, Blute V, Taylor C, Ghosh S, Price T
    Mol Pain, 2013;9(0):12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  15. Glucocorticoid receptors recruit the CaMKIIalpha-BDNF-CREB pathways to mediate memory consolidation.
    Authors: Chen D, Bambah-Mukku D, Pollonini G, Alberini C
    Nat Neurosci, 2012;15(12):1707-14.
    Species: Rat
    Sample Type: In Vivo
    Application: Bioassay
  16. Inhibition of uterine sarcoma cell growth through suppression of endogenous tyrosine kinase B signaling.
    Authors: Makino, Kenichi, Kawamura, Kazuhiro, Sato, Wataru, Kawamura, Nanami, Fujimoto, Toshio, Terada, Yukihiro
    PLoS ONE, 2012;7(7):e41049.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Brain-derived neurotrophic factor ameliorates brain stem cardiovascular dysregulation during experimental temporal lobe status epilepticus.
    Authors: Tsai CY, Chan JY, Hsu KS
    PLoS ONE, 2012;7(3):e33527.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  18. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: N/A
    Sample Type: Protein
    Application: Surface Plasmon Resonance
  19. Brain-derived neurotrophic factor modulates antiretroviral-induced mechanical allodynia in the mouse.
    Authors: Renn CL, Leitch CC, Lessans S, Rhee P, McGuire WC, Smith BA, Traub RJ, Dorsey SG
    J. Neurosci. Res., 2011;89(10):1551-65.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  20. Peptides derived from the solvent-exposed loops 3 and 4 of BDNF bind TrkB and p75(NTR) receptors and stimulate neurite outgrowth and survival.
    Authors: Fobian K, Owczarek S, Budtz C
    J. Neurosci. Res., 2010;88(6):1170-81.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  21. BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
    Int. J. Oncol., 2010;36(6):1469-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Early exercise in spinal cord injured rats induces allodynia through TrkB signaling.
    Authors: Endo T, Ajiki T, Inoue H, Kikuchi M, Yashiro T, Nakama S, Hoshino Y, Murakami T, Kobayashi E
    Biochem. Biophys. Res. Commun., 2009;381(3):339-44.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  23. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008;76(8):3530-8.
    Species: Bacteria
    Sample Type: Trypanosoma
    Application: PcIP
  24. Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions.
    Authors: Fitzgerald M, Buckley A, Lukehurst SS, Dunlop SA, Beazley LD, Rodger J
    Biochem. Biophys. Res. Commun., 2008;374(4):625-30.
  25. Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity: a role for microglia.
    Authors: Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO
    Eur. J. Neurosci., 2008;27(8):2089-102.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. BDNF-exercise interactions in the recovery of symmetrical stepping after a cervical hemisection in rats.
    Authors: Ying Z, Roy RR, Zhong H, Zdunowski S, Edgerton VR, Gomez-Pinilla F
    Neuroscience, 2008;155(4):1070-8.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  27. Brain-derived neurotrophic factor functions as a metabotrophin to mediate the effects of exercise on cognition.
    Authors: Gomez-Pinilla F, Vaynman S, Ying Z
    Eur. J. Neurosci., 2008;28(11):2278-87.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  28. BDNF and learning: Evidence that instrumental training promotes learning within the spinal cord by up-regulating BDNF expression.
    Authors: Gomez-Pinilla F, Huie JR, Ying Z, Ferguson AR, Crown ED, Baumbauer KM, Edgerton VR, Grau JW
    Neuroscience, 2007;148(4):893-906.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  29. Exercise normalizes levels of MAG and Nogo-A growth inhibitors after brain trauma.
    Authors: Chytrova G, Ying Z, Gomez-Pinilla F
    Eur. J. Neurosci., 2007;27(1):1-11.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  30. Exercise differentially regulates synaptic proteins associated to the function of BDNF.
    Authors: Vaynman SS, Ying Z, Yin D, Gomez-Pinilla F
    Brain Res., 2006;1070(1):124-30.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  31. Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
    Authors: Esper RM, Loeb JA
    J. Neurosci., 2004;24(27):6218-27.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 31 Citations
Primary Antibodies
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His Tag Antibody

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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 2 applications: In vivo study, Apoptosis assay.
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 In vivo study Anonymous 07/18/2016
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ApplicationIn vivo study
  Very Good
 Apoptosis assay Anonymous 05/18/2016
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ApplicationApoptosis assay
Reason for Ratingit blocks the activation of TrkB via BDNF.

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