Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen (1). Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).
Recombinant Human Trypsin 1/PRSS1 Protein, CF
R&D Systems | Catalog # 3848-SE
Loading...
Key Product Details
- R&D Systems NS0-derived Recombinant Human Trypsin 1/PRSS1 Protein (3848-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro form
Applications
Enzyme Activity
Loading...
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Trypsin 1/PRSS1 protein
Ala16-Ser247, with a C-terminal 10-His tag
Ala16-Ser247, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala16
Predicted Molecular Mass
26 kDa
SDS-PAGE
32 kDa and 36 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
3848-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HCl and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Trypsin 1/PRSS1
References
- Emi, M. et al. (1986) Gene 41:305.
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
- Teich, N. et al. (2006) Hum. Mutat. 27:721.
Alternate Names
PRSS1, PTRYI, TRY4, Trygn16, Trypsinogen 1
Gene Symbol
PRSS1
UniProt
Additional Trypsin 1/PRSS1 Products
Product Documents for Recombinant Human Trypsin 1/PRSS1 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Trypsin 1/PRSS1 Protein, CF
For research use only
Related Research Areas
Customer Reviews for Recombinant Human Trypsin 1/PRSS1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Trypsin 1/PRSS1 Protein, CF and earn rewards!
Have you used Recombinant Human Trypsin 1/PRSS1 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
View specific protocols for Recombinant Human Trypsin 1/PRSS1 Protein, CF (3848-SE):
Materials
- Activation Buffer: 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
- Assay Buffer: 100 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 8.0
- Recombinant Human Trypsin 1/PRSS1 (rhTrypsin 1) (Catalog # 3848-SE)
- Recombinant Human Enteropeptidase/Enterokinase (rhEnterokinase) (Catalog # 1585-SE)
- Bacterial Thermolysin (Thermolysin) (Catalog # 3097-ZN)
- Substrate: MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 (Catalog # ES002) 2 mM stock in DMSO
- 1,10 Phenanthroline (Sigma, Catalog # 320056), 0.6 M in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Activate rhEnterokinase (see also R&D Systems, Catalog # 1585-SE).
- Activate rhEnterokinase at 50 µg/mL with 1.58 µg/mL Thermolysin in Activation Buffer.
- Incubate at 37 °C for 30 minutes.
- Stop the reaction with an equal volume of 20 mM of 1,10 Phenanthroline for a final concentration of 10 mM.
- Activate rhTrypsin 1 at 100 µg/mL with activated rhEnterokinase at 0.4 µg/mL.
- Dilute the activated rhEnterokinase to 0.8 µg/mL in Assay Buffer.
- Dilute rhTrypsin 1 to 200 µg/mL in Assay Buffer.
- Mix equal volumes of 0.8 µg/mL rhEnterokinase with 200 µg/mL rhTrypsin 1.
- Incubate reaction at room temperature for 15 minutes.
- Dilute activated rhTrypsin 1 to 0.1 µg/mL in Assay Buffer.
- Dilute Substrate to 20 µM in Assay Buffer.
- In a plate load 50 µL of 0.1 µg/mL rhTrypsin 1 to wells, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:
- rhTrypsin 1: 0.005 µg
- Substrate: 10 µM
Loading...