Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).
Recombinant Human Trypsin 2/PRSS2 Protein, CF
R&D Systems | Catalog # 3586-SE
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Key Product Details
- R&D Systems NS0-derived Recombinant Human Trypsin 2/PRSS2 Protein (3586-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro form
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Trypsin 2/PRSS2 protein
Ala16-Ser247, with a C-terminal 10-His tag
Ala16-Ser247, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala16
Predicted Molecular Mass
26 kDa
SDS-PAGE
34 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 3586-SE in the following applications:
Formulation, Preparation, and Storage
3586-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HCl and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Trypsin 2/PRSS2
References
- Emi, M. et al. (1986) Gene. 41:305.
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
- Rinderknecht, H. et al. (1979) Gut. 20:886.
Alternate Names
PRSS2, Tesp4, TRY8, Trypsinogen 20
Gene Symbol
PRSS2
UniProt
Additional Trypsin 2/PRSS2 Products
Product Documents for Recombinant Human Trypsin 2/PRSS2 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Trypsin 2/PRSS2 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Trypsin 2/PRSS2 Protein, CF
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Application: Immunoassay StandardVerified Customer | Posted 12/05/2018We started with only IRT-2 and then added a combination of IRT-1 + IRT-2. Both with excellent results.
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Protocols
View specific protocols for Recombinant Human Trypsin 2/PRSS2 Protein, CF (3586-SE):
Materials
- Activation Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
- Assay Buffer: 0.1 M Tris, 0.15 M NaCl, 10 mM CaCl2, 0.05% Brij-35 (w/v), pH 8.0
- Recombinant Human Enteropeptidase/Enterokinase (rhEnterokinase) (Catalog # 1585-SE)
- Recombinant Human Trypsin 2/PRSS2 (rhTrypsin 2) (Catalog # 3586-SE)
- Bacterial Thermolysin (Thermolysin) (Catalog # 3097-ZN)
- 1,10 Phenanthroline (Sigma, Catalog # 320056), 0.6 M stock in DMSO
- Substrate II: MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH2 (Catalog # ES002), 2 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
Activate rhEnterokinase with Thermolysin.
- Dilute rhEnterokinase to 100 µg/mL in Activation Buffer.
- Dilute Thermolysin to 3.16 µg/mL in Activation Buffer.
- Mix 50 µL of 100 µg/mL rhEnterokinase with 50 µL of 3.16 µg/mL Thermolysin.
- Incubate reaction at 37 °C for 30 minutes.
- Stop reaction by adding 100 µL of 20 mM 1, 10 Phenanthroline to the reaction.
Activate rhTrypsin 2 with activated rhEnterokinase.
- Dilute activated rhEnterokinase to 0.4 µg/mL in Assay Buffer.
- Dilute rhTrypsin 2 to 200 µg/mL in Assay Buffer.
- Mix 25 µL of 0.4 µg/mL rhEnterokinase with 25 µL of 200 µg/mL rhTrypsin 2.
- Incubate reaction at room temperature for 30 minutes.
Perform reaction.
- Dilute activated rhTrypsin 2 to 0.05 µg/mL in Assay Buffer.
- Dilute substrate to 20 µM in Assay Buffer.
- In a plate load 50 µL of 0.05 μg/mL of rhTrypsin 2 and include a Substrate Blank of 50 µL Assay Buffer.
- Start the reaction by adding 50 µL of 20 µM Substrate to wells.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:
- rhTrypsin 2: 0.0025
- Substrate: 10 μM
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