Recombinant Human Wnt-4 Protein

Discontinued Product

6076-WN has been discontinued.
View all Wnt-4 products.
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)

Recombinant Human Wnt-4 Protein Summary

Product Specifications

>60%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The typical ED50 for this effect is 20-100 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human Wnt-4 protein
Accession #
N-terminal Sequence
Structure / Form
Predicted Molecular Mass
39.1 kDa
42-80 kDa, reducing conditions

Product Datasheets

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6076-WN (with carrier)

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6076-WN/CF (carrier free)

Discontinued Product


Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Wnt-4

Wnt-4 is a 38-42 kDa member of the Wnt family of secreted glycoproteins, which act as short-range signaling molecules via Frizzled receptors and a cascade of intracellular signals in vertebrate embryogenesis (1-2). Human Wnt-4 is synthesized as a 351 amino acid (aa) precursor with a 22 aa signal sequence and a 329 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. Relative to other members of the Wnt family, Wnt-4 contains 83 conserved aa, including 21 cysteines (1). Mature human Wnt-4 shares 99%, 98% and 99% aa sequence identity with mature mouse, rat and canine Wnt-4, respectively. Wnt-4 has been shown to play a critical role in the development of the reproductive system and in the formation of the kidneys, adrenals, pituitary gland, and mammary tissues (3-6). In the development of the reproductive system, Wnt-4 expression is down‑regulated in the developing gonad after E11.5, although it persists in the developing ovary (2, 6). Targeted deletion of Wnt-4 results in masculinization of XX mice, with rudimentary development of the masculine internal (Wolffian) ducts and degeneration of the female (Mullerian) reproductive tract (2, 6). In addition to its involvement in urogenital development, Wnt-4 is also expressed in the perichondrium of the long bones (7), and promotes osteoblast differentiation (8). Wnt-4 may also be associated with abnormal proliferation in human breast tissue (9). In humans, mutations in Wnt-4 are the cause of SERKAL syndrome, a syndrome consisting of female to male sex reversal, renal, adrenal, and lung dysgenesis, and developmental defects (3), and Rokitansky-Kuster-Hauser syndrome, which is characterized by utero-vaginal atresia in otherwise phenotypically normal females with normal 46, XX karyotype (10).

  1. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  2. Jordan, B.K. et al. (2001) Am. J. Hum. Genet. 68:1102.
  3. Mandel, H. et al. (2008) Am. J. Hum. Genet. 82:39.
  4. Bernard, P. and V.R. Harley (2007) Int. J. Biochem. Cell Biol. 39:31.
  5. Kuulasmaa, T. et al. (2008) Horm. Metab. Res. 40:668.
  6. Vainio, S. et al. (1999) Nature 397:405.
  7. Gao, X. (2004) Genes Dev. 18:2404.
  8. Chang, J. et al. (2007) J. Biol. Chem. 282:30938.
  9. Huguet, E.L. et al. (1994) Cancer Res. 54:2615.
  10. Philibert, P. et al. (2008) J. Clin. Endocrinol. Metab. 93:895.
Long Name
Wingless-type MMTV Integration Site Family, Member 4
Entrez Gene IDs
54361 (Human); 22417 (Mouse)
Alternate Names
protein Wnt-4; SERKAL; wingless-type MMTV integration site family, member 4; Wnt4; Wnt-4

Citations for Recombinant Human Wnt-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Wnt4 is heterogeneously activated in maturing beta-cells to control calcium signaling, metabolism and function
    Authors: K Katsumoto, S Yennek, C Chen, LFD Silva, S Traikov, D Sever, A Azad, J Shan, S Vainio, N Ninov, S Speier, A Grapin-Bot
    Nature Communications, 2022;13(1):6255.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo
  2. Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis
    Authors: N Yucer, R Ahdoot, MJ Workman, AH Laperle, MS Recouvreux, K Kurowski, DJ Naboulsi, V Liang, Y Qu, JT Plummer, SA Gayther, S Orsulic, BY Karlan, CN Svendsen
    Cell Reports, 2021;37(13):110146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium
    Authors: N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen
    Sci Rep, 2017;7(1):10741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Identification of proliferative and mature ?-cells in the islets of Langerhans
    Nature, 2016;535(7612):430-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
    Authors: Kilander M, Dahlstrom J, Schulte G
    Cell Signal, 2014;26(9):1943-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
    Authors: Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A
    BMC Cancer, 2013;13(0):557.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways.
    Authors: Thrasivoulou C, Millar M, Ahmed A
    J Biol Chem, 2013;288(50):35651-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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