Recombinant Mouse ALCAM His-tag Protein, CF

Catalog # Availability Size / Price Qty
10012-AL-100
Recombinant Mouse ALCAM His-tag Protein, CF Binding Activity
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Recombinant Mouse ALCAM His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse ALCAM/CD166 is coated at 1 µg/mL, Recombinant Biotinylated Human CD6 Fc Chimera binds with an ED50 of 6‑36 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse ALCAM/CD166 protein
Trp28-Lys527, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Trp28
Predicted Molecular Mass
57 kDa
SDS-PAGE
81-95 kDa, reducing conditions

Product Datasheets

10012-AL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Mouse ALCAM His-tag Protein, CF Binding Activity View Larger

When Recombinant Mouse ALCAM/CD166 is immobilized at 1 μg/mL, RecombinantBiotinylated Human CD6 Fc Chimera binds with an ED50 of 6-36 ng/mL.

SDS-PAGE Recombinant Mouse ALCAM His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse ALCAM/CD166 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 81-95  kDa.

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Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166 and also called MEMD and SC-1/DM-GRASP/BEN in the chicken, is a 100-110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte ­derived dendritic cells (1, 2). Mouse ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V-type and three C2-type Ig-like domains, transmembrane (22 aa) and cytoplasmic (34 aa) domains (1). Mouse ALCAM ECD shares 98%, 93%, and 92% aa sequence identity with rat, human/porcine and bovine/equine ALCAM, respectively. A secreted isoform in endothelial cells that is truncated at aa 133 (sALCAM) antagonizes full‑length ALCAM (3, 4). ALCAM mediates low affinity adhesion with itself or the cysteine-rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1-11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM-expressing antigen presenting cells and CD6-expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (2, 7, 8). High ALCAM expression at the blood brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on melanoma cell lines appears to be pro-metastatic, but anti-metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM-L1, both in the developing retina and invasive melanoma (3, 12). It may also play a role in stabilizing cell-cell interaction among lymphatic endothelial cells (LECs) and affecting the organization and function of the lymphatic vessel (LV) network (13).

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  3. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
  4. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
  5. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
  6. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
  7. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
  8. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  9. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  10. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  11. King, J.A. et al. (2010) Mol. Cancer 9:266.
  12. Buhusi, M. et al. (2009) J. Neurosci. 29:15630.
  13. Iolyeva M. et al. (2013) FASEB J. 27:978.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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