Recombinant Mouse CCL17/TARC Protein

Name: Recombinant Mouse CCL17/TARC Protein
Brand: R&D Systems
SKU: 529-TR
Price: 292 USD
Availability: InStock
Rating: 4 (1 reviews)

R&D Systems | Catalog # 529-TR

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Citations (17)

Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems E. coli-derived Recombinant Mouse CCL17/TARC Protein (529-TR)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q9WUZ6

Applications

Bioactivity

View all CCL17/TARC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse CCL17/TARC protein
Ala24-Pro93

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

7.9 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR4.
The ED₅₀ for this effect is 0.300-3.00 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 529-TR in the following applications:

Immunoassay Standard (1 Review)

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 529-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 529-TR/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CCL17/TARC

Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse TARC was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse TARC cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, TARC has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. The gene for human TARC has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse TARC is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant TARC has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, TARC was identified to be a specific functional ligand for CCR-4 and CCR-8, receptors that are selectively expressed on T cells.