Recombinant Mouse CD19 Fc Chimera Protein, CF

R&D Systems | Catalog # 9730-CD

R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse CD19 Fc Chimera Protein (9730-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P25918

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD19 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CD19 protein
Mouse CD19
(Arg19-Gly287)
Accession # P25918		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg19 and Ser68

Predicted Molecular Mass

57 kDa

SDS-PAGE

78-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD19 Fc Chimera is immobilized at 2 µg/mL,100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED50 of 7-35 μg/mL

Reviewed Applications

Read 3 reviews rated 5 using 9730-CD in the following applications:

Formulation, Preparation, and Storage

9730-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker (1). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (4, 5). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13, (6-9). These associations enable CD19 to amplify B cell signaling (7, 9-12) and reduce the threshold for antigen stimulation through the BCR (13). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers. The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials. Mature human CD19 consists of a 272 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 22 aa transmembrane segment, and a 243 aa cytoplasmic domain (3). Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19.

References

  1. Wang, K. et al. (2012) Exp. Hematol. Oncol. 1:36.
  2. Tedder, T.F. et al. (1997) Immunity 6:107.
  3. Tedder, T.F. and C.M. Isaacs (1989) J. Immunol. 143:712.
  4. Rickert, R.C. et al. (1995) Nature 376:352.
  5. Engel, P. et al. (1995) Immunity 3:39.
  6. Vences-Catalan, F. et al. (2012) J. Immunol. 137:48.
  7. Deaglio, S. et al. (2007) Blood 109:5390.
  8. Bradbury, L. et al. (1992) J. Immunol. 149:2841.
  9. Krop, I. et al. (1996) J. Immunol. 157:48.
  10. O'Rourke, L.M. et al. (1998) Immunity 8:635.
  11. Buhl, A.M. et al. (1997) J. Exp. Med. 186:1897.
  12. Sato, S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11558.
  13. Carter, R.H. and D.T. Fearon (1992) Science 256:105.

Alternate Names

CD19, CVID3, Leu-12

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Gene Symbol

CD19

UniProt

P25918

Additional CD19 Products

Product Documents for Recombinant Mouse CD19 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse CD19 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse CD19 Fc Chimera Protein, CF

Customer Reviews for Recombinant Mouse CD19 Fc Chimera Protein, CF (3)

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  • Recombinant Mouse CD19 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/07/2025
  • Recombinant Mouse CD19 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/24/2020
  • Recombinant Mouse CD19 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Cell Proliferation
    Verified Customer | Posted 05/18/2020

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