Recombinant Mouse CTLA-4 Fc Chimera Protein, CF

  (10 citations)     
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Citations (10)
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  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.1 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED50 for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1 Fc Chimera (Catalog # 140-B1) in the presence of PHA.
  • Source
    Mouse myeloma cell line, NS0-derived mouse CTLA-4 protein
    Mouse CTLA-4
    Ala37 - Phe162
    Accession #Q6GTR6
    IEGRMD Human IgG1
    (Pro100 - Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala37
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    41 kDa (monomer)
  • SDS-PAGE
    55 kDa, reducing conditions
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
434-CT/CF
 
434-CT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
 
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CTLA-4

CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41 ‑ 43 kDa (2) Within the ECD, mouse CTLA-4 shares 94% and 68 ‑ 71% aa sequence identity with rat and human/porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3 ‑ 5). Isoforms of 56 ‑ 79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11 ‑ 13).

  • References:
    1. Harper, K. et al. (1991) J. Immunol. 147:1037.
    2. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65.
    3. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596.
    4. Tector, M. et al. (2009) BMC Immunol. 10:51.
    5. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015.
    6. Vijayakrishnan, L. et al. (2004) Immunity 20:563.
    7. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
    8. Jansson, A. et al. (2005) J. Immunol 175:1575.
    9. Darlington, P.J. et al. (2005) J. Immunol. 175:996.
    10. Platt, A.M. et al. (2010) J. Immunol. 185:1558.
    11. Wing, K. et al. (2008) Science 322:271.
    12. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421.
    13. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1524.
  • Long Name:
    Cytotoxic T-lymphocyte-associated Molecule 4
  • Entrez Gene IDs:
    1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
  • Alternate Names:
    CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells.
    Authors: Stojanovic A, Fiegler N, Brunner-Weinzierl M, Cerwenka A
    J Immunol, 2014;192(9):4184-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Blocking
  2. Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3.
    Authors: Kowalczyk A, D'Souza C, Zhang L
    Eur J Immunol, 2014;44(4):1143-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Local CTLA4 blockade effectively restrains experimental pancreatic adenocarcinoma growth in vivo.
    Authors: Sandin L, Eriksson F, Ellmark P, Loskog A, Totterman T, Mangsbo S
    Oncoimmunology, 2014;3(1):e27614.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Standard
  4. CD86 is an activation receptor for NK cell cytotoxicity against tumor cells.
    Authors: Peng, Yanmeng, Luo, Gaoxing, Zhou, Junyi, Wang, Xiaojuan, Hu, Jie, Cui, Yanyan, Li, Xian C, Tan, Jianglin, Yang, Sisi, Zhan, Rixing, Yang, Junjie, He, Weifeng, Wu, Jun
    PLoS ONE, 2013;8(12):e83913.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  5. Dendritic cell modification as a route to inhibiting corneal graft rejection by the indirect pathway of allorecognition.
    Authors: Khan A, Fu H, Tan L, Harper J, Beutelspacher S, Larkin D, Lombardi G, McClure M, George A
    Eur J Immunol, 2013;43(3):734-46.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. TIM-3 does not act as a receptor for galectin-9.
    Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
    PLoS Pathog, 2013;9(3):e1003253.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
    Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
    Immunity, 2007;27(1):111-22.
    Species: N/A
    Sample Type: N/A
    Application: Bioassay
  8. Induction of CTLA-4-mediated anergy contributes to persistent colonization in the murine model of gastric Helicobacter pylori infection.
    Authors: Anderson KM, Czinn SJ, Redline RW, Blanchard TG
    J. Immunol., 2006;176(9):5306-13.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA.
    Authors: La Cava A, Ebling FM, Hahn BH
    J. Immunol., 2004;173(5):3542-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Marked prolongation of cardiac allograft survival by dendritic cells genetically engineered with NF-kappa B oligodeoxyribonucleotide decoys and adenoviral vectors encoding CTLA4-Ig.
    Authors: Bonham CA, Peng L, Liang X, Chen Z, Wang L, Ma L, Hackstein H, Robbins PD, Thomson AW, Fung JJ, Qian S, Lu L
    J. Immunol., 2002;169(6):3382-91.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Standard

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