Recombinant Mouse DC-SIGN/CD209 Protein, CF

Name: Recombinant Mouse DC-SIGN/CD209 Protein, CF
Brand: R&D Systems
SKU: 8345-DC
Price: 454 USD
Availability: InStock

R&D Systems | Catalog # 8345-DC

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Mouse DC-SIGN/CD209 Protein (8345-DC)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q91ZX1

Applications

Bioactivity

View all DC-SIGN/CD209 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DC-SIGN/CD209 protein
Val73-Lys238, with an N-terminal HA tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr (HA tag)

Predicted Molecular Mass

20 kDa

SDS-PAGE

21-26 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse DC-SIGN is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICAM-5 Fc Chimera (Catalog # 1173-M5) that produces 50% optimal binding response is 0.8-4 μg/mL.

Formulation, Preparation, and Storage

8345-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).