Recombinant Mouse Dkk-1 (Histidine-tagged) Protein

Newer Version Available: 5897-DK-010
A New and Improved rm Dkk-1 is Available! It has a lower endotoxin specification, ~5 fold better activity, and no tag!
Formulations:
Catalog # Availability Size / Price Qty
1765-DK-010
Recombinant Mouse Dkk-1 (Histidine-tagged) Protein Bioactivity
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Product Details
Citations (7)
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Recombinant Mouse Dkk-1 (Histidine-tagged) Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 30-180 ng/mL. Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ND50 for this effect is 0.2-1 μg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Mouse myeloma cell line, NS0-derived mouse Dkk-1 protein
Ser30-His272, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Ser30
Predicted Molecular Mass
27.6 kDa
SDS-PAGE
38 kDa and 41 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1765-DK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1765-DK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Dkk-1 (Histidine-tagged) Protein Bioactivity View Larger

Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765‑DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is
30‑180 ng/mL (orange circles).

Reconstitution Calculator

Reconstitution Calculator

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Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

References
  1. Glinka, A. et al. (1998) Nature 391:357.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
Long Name
Dickkopf-1
Entrez Gene IDs
22943 (Human); 13380 (Mouse)
Alternate Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

Citations for Recombinant Mouse Dkk-1 (Histidine-tagged) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity.
    Authors: Trowbridge JJ, Guezguez B, Moon RT
    Stem Cells, 2010;28(8):1379-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
    Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
    Dev. Biol., 2009;330(2):286-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium.
    Authors: Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M
    FASEB J., 2009;23(1):114-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors.
    Authors: Kwon C, Arnold J, Hsiao EC, Taketo MM, Conklin BR, Srivastava D
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(26):10894-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Wnt3a binds to several sFRPs in the nanomolar range.
    Authors: Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L
    Biochem. Biophys. Res. Commun., 2007;357(4):1119-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Very low density lipoprotein receptor, a negative regulator of the wnt signaling pathway and choroidal neovascularization.
    Authors: Chen Y, Hu Y, Lu K, Flannery JG, Ma JX
    J. Biol. Chem., 2007;282(47):34420-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006;133(19):3787-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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