Recombinant Mouse Dkk-2 Protein

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a competitive binding assay. When Recombinant Mouse Kremen‑1 (Catalog # 1647-KR) is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse Dkk-2 inhibits 50% binding of biotinylated Recombinant Human Dkk-1 (Catalog # 5439-DK) (200 ng/mL) at the concentration range of 0.25-1.5 µg/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived Ser26-Ile259, with Leu35Pro substitution and a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser26
  • Predicted Molecular Mass
    27 kDa
  • SDS-PAGE
    29-35 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2435-DKB
 
2435-DKB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
RecombinantMouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wntreporter activity in HEK293 cells (green circles). Recombinant Mouse DKK-2(Catalog # 2435-DKB) inhibits a constant dose of 100 ng/ml of Recombinant MouseWnt-3a. The ED50 for this effect is100-600 ng/ml (orange circles).
When Recombinant Mouse Kremen-1 (Catalog # 1647-KR) is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse Dkk-2 (Catalog #
2435-DKB) inhibits 50% binding of biotinylated Recombinant Human Dkk-1 (Catalog # 5439-DK) (200 ng/mL) at the concentration range of
0.25-1.5 µg/mL.
Background: Dkk-2

Dickkopf related protein 2 (Dkk-2) is a member of the Dickkopf family of secreted Wnt modulators (1-3). Dkk proteins contain a signal peptide and two conserved cysteine-rich domains that are separated by a linker region. The second cysteine-rich domain mediates Dkk-2 binding activities, and its interaction with LRP beta propellers has been mapped (2-4, 7). The 226 aa, ~35 kDa mature mouse Dkk-2 shares  99%, 96%, 96%, 96% and 94% aa identity with rat, human, dog, horse and cow Dkk-2, respectively, and can activate the canonical Wnt signaling pathway in Xenopus embryos (5). Dkk proteins modify Wnt engagement of a receptor complex composed of a Frizzled protein and a low-density lipoprotein receptor-related protein, either LRP5 or LRP6 (3). When LRP6 is over-expressed, direct high-affinity binding of Dkk-2 to LRP can enhance canonical Wnt signaling (6-8). However, when Dkk-2 and LRP6 form a ternary complex with Kremen2, Wnt signaling is inhibited due to internalization of Dkk-2/LRP6/Krm2 complexes (9, 10). Thus, depending on the cellular context, Dkk-2 can either activate or inhibit canonical Wnt signaling (3). In contrast, binding of Dkk-1 or Dkk-4 to LRP is consistently antagonistic (3). Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations. Dkk-2 expression has been studied most in bone and eye, although it is expressed as early as periimplantation in mice (11). Mouse Dkk-1 or Dkk-2 deficiencies have opposite effects on bone homeostasis, despite down-regulating Wnt antagonism in both cases (12, 13). Dkk-2 expression is induced by Wnts in bone, and is thought to enhance bone density by promoting terminal differentiation of osteoblasts and mineral deposition (12). In contrast, Dkk-1 negatively regulates late osteoblast proliferation, which limits bone density (13). Dkk-2-deficient mice are blind, exhibiting faulty differentiation of corneal epithelium and ectopic blood vessels in the periocular mesenchyme (14, 15).

  • References:
    1. Monaghan, A.P. et al. (1999) Mech. Dev. 87:45.
    2. Krupnik, V.E. et al. (1999) Gene 238:301.
    3. Niehrs, C. (2006) Oncogene 25:7469.
    4. Chen, L. et al. (2008) J. Biol. Chem. 283:23364.
    5. Wu, W. et al. (2000) Current Biol. 10:1611.
    6. Mao, B. et al. (2001) Nature 411:321.
    7. Li, L. et al. (2002) J. Biol. Chem. 277:5977.
    8. Brott, B. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
    9. Mao, B. et al. (2002) Nature 417:664.
    10. Mao, B. and C. Niehrs (2003) Gene 302:179.
    11. Zhang, Y. et al. (2009) J. Reprod. Dev. 55:17.
    12. Li, X. et al. (2005) Nat. Genet. 37:945.
    13. van der Horst, G. et al. (2005) J. Bone Miner. Res. 20:1867.
    14. Mukhopadhyay, M. et al. (2006) Development 133:2149.
    15. Gage, P.J. et al. (2008) Dev. Biol. 317:310.
  • Long Name:
    Dickkopf-2
  • Entrez Gene IDs:
    27123 (Human); 56811 (Mouse)
  • Alternate Names:
    dickkopf (Xenopus laevis) homolog 2; dickkopf 2; dickkopf homolog 2 (Xenopus laevis); dickkopf related protein-2; dickkopf-2; dickkopf-related protein 2; Dkk2; Dkk-2; hDkk-2
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Biotinylated Antibody

WB BAM050 3  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human Dkk-1 Protein, CF

BA, BA2 5439-DK/CF 34  
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Recombinant Human Dkk-1 Protein

BA2 5439-DK 34  
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Recombinant Mouse Kremen-1 Protein, CF

Bind 1647-KR/CF
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Recombinant Mouse Kremen-1 Protein

Bind 1647-KR
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