Recombinant Human Dkk-1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
5439-DK-010/CF
5439-DK-100/CF
5439-DK-01M/CF

With Carrier

Catalog # Availability Size / Price Qty
5439-DK-010
Recombinant Human Dkk-1 Protein Bioactivity
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Product Details
Citations (80)
FAQs
Reviews (5)

Recombinant Human Dkk-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Human Dkk-1 (Catalog # 5439-DK) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt‑3a (Catalog # 5036‑WN). The ED50 for this effect is 10-60 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Dkk-1 protein
Thr32-His266
Accession #
N-terminal Sequence
Analysis
Thr32
Predicted Molecular Mass
25.8 kDa
SDS-PAGE
33-38 kDa, reducing conditions

Product Datasheets

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5439-DK (with carrier)

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5439-DK/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5439-DK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5439-DK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Dkk-1 Protein Bioactivity View Larger

Recombinant Human Wnt-3a (Catalog # 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Human Dkk-1 (Catalog # 5439-DK) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a. The ED50 for this effect is 10-60 ng/mL (orange circles).

SDS-PAGE Recombinant Human Dkk-1 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 33-38 kDa. Multiple bands in gel are due to variable glycosylation.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

References
  1. Krupnik, V.E. et al. (1999) Gene 238:301.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
Long Name
Dickkopf-1
Entrez Gene IDs
22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
Alternate Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

Citations for Recombinant Human Dkk-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

80 Citations: Showing 1 - 10
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  1. miR-181-5p attenuates neutrophilic inflammation in asthma by targeting DEK
    Authors: Y Song, Z Wang, J Jiang, Y Piao, Q Bai, Q Piao, L Li, C Xu, H Liu, H Piao, L Li, G Yan
    International immunopharmacology, 2022;112(0):109243.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells
    Authors: C Kornsuthis, A Chansaenro, J Manokawinc, KA Tompkins, N Pirarat, T Osathanon
    Scientific Reports, 2022;12(1):7583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium
    Authors: TF Amaral, JGV de Grazia, LAG Martinhao, F De Col, LGB Siqueira, JHM Viana, PJ Hansen
    Scientific Reports, 2022;12(1):7503.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Epithelial plasticity in COPD results in cellular unjamming due to an increase in polymerized actin
    Authors: B Ghosh, K Nishida, L Chandrala, S Mahmud, S Thapa, C Swaby, S Chen, AA Khosla, J Katz, VK Sidhaye
    Journal of Cell Science, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  5. Effects of baicalin on the proliferation and expression of OPG and RANKL in human cementoblast-lineage cells
    Authors: R Kunimatsu, A Kimura, S Sakata, Y Tsuka, Y Yoshimi, T Abe, I Kado, Y Yashima, J Izumino, A Nakatani, M Kitagawa, M Miyauchi, T Takata, K Tanimoto
    Journal of dental sciences, 2022;17(1):162-169.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Dickkopf-1 Promotes Angiogenesis and is a Biomarker for Hepatic Stem Cell-like Hepatocellular Carcinoma
    Authors: T Suda, T Yamashita, H Sunagozaka, H Okada, K Nio, Y Sakai, T Yamashita, E Mizukoshi, M Honda, S Kaneko
    International Journal of Molecular Sciences, 2022;23(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Combination of Wnt/beta-Catenin Targets S100A4 and DKK1 Improves Prognosis of Human Colorectal Cancer
    Authors: M Dahlmann, A Monks, ED Harris, D Kobelt, M Osterland, F Khaireddin, P Herrmann, W Kemmner, S Burock, W Walther, RH Shoemaker, U Stein
    Cancers, 2021;14(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain
    Authors: M Kowalski-J, H Schihada, A Turku, T Huber, TP Sakmar, G Schulte
    Science Advances, 2021;7(46):eabj7917.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs
    Authors: L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine
    International Journal of Molecular Sciences, 2021;22(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Topological isolation of developmental regulators in mammalian genomes
    Authors: HJ Wu, A Landshamme, EK Stamenova, A Bolondi, H Kretzmer, A Meissner, F Michor
    Nature Communications, 2021;12(1):4897.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells
    Authors: D Baek, KH Park, KM Lee, S Jung, S Joung, J Kim, JW Lee
    Cell Death & Disease, 2021;12(3):238.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Wnt/&beta-catenin signaling mediates the abnormal osteogenic and adipogenic capabilities of bone marrow mesenchymal stem cells from chronic graft-versus-host disease patients
    Authors: HZ Qi, YL Ye, Y Suo, H Qu, HY Zhang, KB Yang, ZP Fan, F Huang, L Xuan, YQ Chen, H Jin, QF Liu
    Cell Death & Disease, 2021;12(4):308.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Overexpressed sFRP3 exerts an inhibitory effect on hepatocellular carcinoma via inactivation of the Wnt/&beta-catenin signaling pathway
    Authors: L Fang, C Gao, RX Bai, HF Wang, SY Du
    Cancer Gene Ther., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Recombinant Klotho protein enhances cholesterol efflux of THP-1 macrophage-derived foam cells via suppressing Wnt/&beta-catenin signaling pathway
    Authors: W Liu, X Chen, M Wu, L Li, J Liu, J Shi, T Hong
    BMC Cardiovasc Disord, 2020;20(1):120.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Norrin mediates tumor-promoting and -suppressive effects in glioblastoma via Notch and WNT
    Authors: A El-Sehemy, HJ Selvadurai, A Ortin-Mart, NT Pokrajac, Y Mamatjan, N Tachibana, KJ Rowland, L Lee, NI Park, KD Aldape, P Dirks, VA Wallace
    J. Clin. Invest., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  16. Wnt16 signaling promotes osteoblast differentiation of periosteal derived cells in vitro and in vivo
    Authors: Y Jin, X Sun, F Pei, Z Zhao, J Mao
    PeerJ, 2020;8(0):e10374.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  17. Elevated Dkk1 Mediates Downregulation of the Canonical Wnt Pathway and Lysosomal Loss in an iPSC Model of Neuronopathic Gaucher Disease
    Authors: MP Srikanth, RA Feldman
    Biomolecules, 2020;10(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Single cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors
    Authors: JM Segal, D Kent, DJ Wesche, SS Ng, M Serra, B Oulès, G Kar, G Emerton, SJI Blackford, S Darmanis, R Miquel, TV Luong, R Yamamoto, A Bonham, W Jassem, N Heaton, A Vigilante, A King, R Sancho, S Teichmann, SR Quake, H Nakauchi, ST Rashid
    Nat Commun, 2019;10(1):3350.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  19. The intracellular Ca2+ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient
    Authors: F Tsurumi, S Baba, D Yoshinaga, K Umeda, T Hirata, J Takita, T Heike
    PLoS ONE, 2019;14(3):e0213768.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett's associated esophageal adenocarcinoma
    Authors: O Lyros, AK Lamprecht, L Nie, R Thieme, K Götzel, M Gasparri, G Haasler, P Rafiee, R Shaker, I Gockel
    Am J Cancer Res, 2019;9(2):330-346.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue
    Authors: OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti
    Nat Protoc, 2019;14(6):1884-1925.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12
    Authors: S Pérez-Garc, M Carrión, R Villanueva, T Hermida-Gó, M Fernández-, M Mellado, FJ Blanco, Y Juarranz, RP Gomariz
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. δN-Tp63 Mediates Wnt/ β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia
    Authors: M Haas, JL Gómez Vázq, DI Sun, HT Tran, M Brislinger, A Tasca, O Shomroni, K Vleminckx, P Walentek
    Cell Rep, 2019;28(13):3338-3352.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. ECHO, the executable CHOndrocyte: A computational model to study articular chondrocytes in healthy and disease
    Authors: S Schivo, S Khurana, K Govindaraj, J Scholma, J Kerkhofs, L Zhong, X Huang, J van de Pol, R Langerak, AJ van Wijnen, L Geris, M Karperien, JN Post
    Cell. Signal., 2019;0(0):109471.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Apelin enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly through Wnt/?-catenin signaling pathway
    Authors: K Hang, C Ye, J Xu, E Chen, C Wang, W Zhang, L Ni, Z Kuang, L Ying, D Xue, Z Pan
    Stem Cell Res Ther, 2019;10(1):189.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling
    Authors: B Cha, X Geng, MR Mahamud, JY Zhang, L Chen, W Kim, EH Jho, Y Kim, D Choi, JB Dixon, H Chen, YK Hong, L Olson, TH Kim, BJ Merrill, MJ Davis, RS Srinivasan
    Cell Rep, 2018;25(3):571-584.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling
    Authors: S Kokabu, V Rosen
    FEBS Open Bio, 2018;8(2):168-176.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype
    Authors: M Amin, Y Kushida, S Wakao, M Kitada, K Tatsumi, M Dezawa
    Cell Transplant, 2018;27(2):285-298.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. VGF nerve growth factor inducible is involved in retinal ganglion cells death induced by optic nerve crush
    Authors: H Takeuchi, S Inagaki, W Morozumi, Y Nakano, Y Inoue, Y Kuse, T Mizoguchi, S Nakamura, M Funato, H Kaneko, H Hara, M Shimazawa
    Sci Rep, 2018;8(1):16443.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Involvement of the capsular GalXM-induced IL-17 cytokine in the control of Cryptococcus neoformans infection
    Authors: IF LaRocque-d, JDB Rocha, MP Nunes, PAV Oliveira, DO Nascimento, L Freire-de-, CM Takiya, A Morrot, D Decote-Ric, JO Previato, GA DosReis, L Mendonça-P, CG Freire-de-
    Sci Rep, 2018;8(1):16378.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. hPSC-Derived Striatal Cells Generated Using a Scalable 3D Hydrogel Promote Recovery in a Huntington Disease Mouse Model
    Authors: MM Adil, T Gaj, AT Rao, RU Kulkarni, CM Fuentes, GN Ramadoss, FK Ekman, EW Miller, DV Schaffer
    Stem Cell Reports, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Identification of DKK-1 as a novel mediator of statin effects in human endothelial cells
    Authors: M Pontremoli, M Brioschi, R Baetta, S Ghilardi, C Banfi
    Sci Rep, 2018;8(1):16671.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5a/?-Catenin Signaling Pathway
    Authors: YK Bae, JH Kwon, M Kim, GH Kim, SJ Choi, W Oh, YS Yang, HJ Jin, HB Jeon
    Stem Cells Int, 2018;2018(0):6545071.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA
    Authors: YC Chen, CL Tsai, YH Wei, YT Wu, WT Hsu, HC Lin, YC Hsu
    Cell Death Dis, 2018;9(4):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Wnt7a Inhibits IL-1? Induced Catabolic Gene Expression and Prevents Articular Cartilage Damage in Experimental Osteoarthritis
    Authors: AL Gibson, CK Hui Mingal, AT Foote, T Uchimura, M Zhang, L Zeng
    Sci Rep, 2017;7(0):41823.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Wnt3a Induces the Expression of Acetylcholinesterase during Osteoblast Differentiation via the Runx2 Transcription Factor
    Authors: ML Xu, CWC Bi, EYL Liu, TTX Dong, KWK Tsim
    J. Biol. Chem., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  37. WNT/?-Catenin signaling pathway regulates non-tumorigenesis of human embryonic stem cells co-cultured with human umbilical cord mesenchymal stem cells
    Authors: YH Chang, TY Chu, DC Ding
    Sci Rep, 2017;7(0):41913.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Novel regulatory mechanisms for the SoxC transcriptional network required for visual pathway development
    Authors: KC Chang, J Hertz, X Zhang, XL Jin, P Shaw, BA Derosa, JY Li, P Venugopala, DA Valenzuela, RD Patel, KR Russano, SA Alshamekh, C Sun, K Tenerelli, C Li, D Velmeshev, Y Cheng, TM Boyce, A Dreyfuss, MS Uddin, KJ Muller, DM Dykxhoorn, JL Goldberg
    J. Neurosci., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells
    Authors: F Ou, K Su, J Sun, W Liao, Y Yao, Y Zheng, Z Zhang
    Biochem. Biophys. Res. Commun., 2017;487(2):457-463.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Transplantation of Human Embryonic Stem Cell-Derived Retinal Cells into the Subretinal Space of a Non-Human Primate
    Authors: JR Chao, DA Lamba, TR Klesert, A Torre, A Hoshino, RJ Taylor, A Jayabalu, AL Engel, TH Khuu, RK Wang, M Neitz, J Neitz, TA Reh
    Transl Vis Sci Technol, 2017;6(3):4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Dickkopf-1 inhibits Wnt3a-induced migration and epithelial-mesenchymal transition of human lens epithelial cells
    Authors: T Liu, L Zhang, Y Wang, H Zhang, L Li, X Bao
    Exp. Eye Res., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Experimental Study of the Biological Properties of Human Embryonic Stem Cell-Derived Retinal Progenitor Cells
    Authors: J Shao, PY Zhou, GH Peng
    Sci Rep, 2017;7(0):42363.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Human MSCs promotes colorectal cancer epithelial-mesenchymal transition and progression via CCL5/?-catenin/Slug pathway
    Authors: K Chen, Q Liu, LL Tsang, Q Ye, HC Chan, Y Sun, X Jiang
    Cell Death Dis, 2017;8(5):e2819.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway
    Authors: Y Gao, A Cai, H Xi, J Li, W Xu, Y Zhang, K Zhang, J Cui, X Wu, B Wei, L Chen
    Stem Cell Res Ther, 2017;8(1):98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Autocrine WNT2 signaling in fibroblasts promotes colorectal cancer progression
    Authors: N Kramer, J Schmöllerl, C Unger, H Nivarthi, A Rudisch, D Unterleuth, M Scherzer, A Riedl, M Artaker, I Crncec, D Lenhardt, T Schwarz, B Prieler, X Han, M Hengstschl, J Schüler, R Eferl, R Moriggl, W Sommergrub, H Dolznig
    Oncogene, 2017;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  46. Attenuation of Dickkopf 1-Induced Hair Growth Inhibition in Cultured Human Hair Follicles by Tianeptine
    Authors: HS Jeong, MH Kwack, MK Kim, JC Kim, YK Sung
    Ann Dermatol, 2017;29(1):102-105.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  47. Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. COX-2 Induces Breast Cancer Stem Cells via EP4/PI3K/AKT/NOTCH/WNT Axis
    Authors: Mousumi Majumder
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Endothelial cells regulate ?-catenin activity in adrenocortical cells via secretion of basic fibroblast growth factor
    Authors: Holger S Willenberg
    Mol. Cell. Endocrinol., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts
    Authors: Elena Neumann
    Matrix Biol., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
    Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
    Nat Commun, 2015;6(0):6156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Influence of Sex on Basal and Dickkopf-1 Regulated Gene Expression in the Bovine Morula.
    Authors: Denicol A, Leao B, Dobbs K, Mingoti G, Hansen P
    PLoS ONE, 2015;10(7):e0133587.
    Species: Bovine
    Sample Types: Whole Tissue
    Applications: Bioassay
  53. Isolation and characterization of embryonic stem cell-derived cardiac Purkinje cells.
    Authors: Maass K, Shekhar A, Lu J, Kang G, See F, Kim E, Delgado C, Shen S, Cohen L, Fishman G
    Stem Cells, 2015;33(4):1102-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  54. A protein kinase A (PKA)/beta-catenin pathway sustains the BMP2/DLX3-induced osteogenic differentiation in dental follicle cells (DFCs).
    Authors: Viale-Bouroncle S, Klingelhoffer C, Ettl T, Reichert T, Morsczeck C
    Cell Signal, 2015;27(3):598-605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Wnt/beta-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway.
    Authors: Peng X, Yang L, Chang H, Dai G, Wang F, Duan X, Guo L, Zhang Y, Chen G
    PLoS ONE, 2014;9(5):e97283.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Inner ear hair cell-like cells from human embryonic stem cells.
    Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
    Stem Cells Dev, 2014;23(11):1275-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. FGF16 promotes invasive behavior of SKOV-3 ovarian cancer cells through activation of mitogen-activated protein kinase (MAPK) signaling pathway.
    Authors: Basu M, Mukhopadhyay S, Chatterjee U, Roy S
    J Biol Chem, 2014;289(3):1415-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype.
    Authors: Burnight E, Wiley L, Drack A, Braun T, Anfinson K, Kaalberg E, Halder J, Affatigato L, Mullins R, Stone E, Tucker B
    Gene Ther, 2014;21(7):662-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
    Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
    PLoS ONE, 2014;9(8):e104937.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Elevated levels of Dickkopf-1 are associated with beta-catenin accumulation and poor prognosis in patients with chondrosarcoma.
    Authors: Chen C, Zhou H, Zhang X, Ma X, Liu Z, Liu X
    PLoS ONE, 2014;9(8):e105414.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer.
    Authors: Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W
    J Biol Chem, 2013;288(36):26301-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Unraveling the signaling pathways promoting fibrosis in Dupuytren's disease reveals TNF as a therapeutic target.
    Authors: Verjee L, Verhoekx J, Chan J, Krausgruber T, Nicolaidou V, Izadi D, Davidson D, Feldmann M, Midwood K, Nanchahal J
    Proc Natl Acad Sci U S A, 2013;110(10):E928-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Screening drug-induced arrhythmia [corrected] using human induced pluripotent stem cell-derived cardiomyocytes and low-impedance microelectrode arrays.
    Authors: Navarrete E, Liang P, Lan F, Sanchez-Freire V, Simmons C, Gong T, Sharma A, Burridge P, Patlolla B, Lee A, Wu H, Beygui R, Wu S, Robbins R, Bers D, Wu J
    Circulation, 2013;128(11):S3-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.
    Authors: Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, Liu B, Goulburn A, Stanley E, Elefanty A, Widmer H, Eggan K, Goldstein P, Anderson S, Studer L
    Cell Stem Cell, 2013;12(5):559-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa.
    Authors: Tucker, Budd A, Mullins, Robert F, Streb, Luan M, Anfinson, Kristin, Eyestone, Mari E, Kaalberg, Emily, Riker, Megan J, Drack, Arlene V, Braun, Terry A, Stone, Edwin M
    Elife, 2013;2(0):e00824.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Drug screening using a library of human induced pluripotent stem cell-derived cardiomyocytes reveals disease-specific patterns of cardiotoxicity.
    Authors: Liang, Ping, Lan, Feng, Lee, Andrew S, Gong, Tingyu, Sanchez-Freire, Veronica, Wang, Yongming, Diecke, Sebastia, Sallam, Karim, Knowles, Joshua W, Wang, Paul J, Nguyen, Patricia, Bers, Donald M, Robbins, Robert C, Wu, Joseph C
    Circulation, 2013;127(16):1677-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium.
    Authors: Buchholz, David E, Pennington, Britney, Croze, Roxanne, Hinman, Cassidy, Coffey, Peter J, Clegg, Dennis O
    Stem Cells Transl Med, 2013;2(5):384-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  68. Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells.
    Authors: Singh A, Chappell J, Trost R, Lin L, Wang T, Tang J, Matlock B, Weller K, Wu H, Zhao S, Jin P, Dalton S
    Stem Cell Reports, 2013;1(6):532-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer.
    Authors: Tahir S, Yang G, Goltsov A, Song K, Ren C, Wang J, Chang W, Thompson T
    Cancer Res, 2013;73(6):1900-11.
  70. Dickkopf 1 promotes regression of hair follicles.
    Authors: Kwack MH, Kim MK, Kim JC, Sung YK
    J. Invest. Dermatol., 2012;132(6):1554-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  71. Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
    Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
    PLoS ONE, 2011;6(1):e16366.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  72. The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring.
    Authors: Chua AW, Ma D, Gan SU, Fu Z, Han HC, Song C, Sabapathy K, Phan TT
    J. Invest. Dermatol., 2011;131(3):644-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  73. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast.
    Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
    Cell, 2011;145(6):926-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  74. Neuroblastoma and bone metastases: clinical significance and prognostic value of Dickkopf 1 plasma levels.
    Authors: Granchi D, Corrias MV, Garaventa A, Baglio SR, Cangemi G, Carlini B, Paolucci P, Giunti A, Baldini N
    Bone, 2011;48(1):152-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  75. Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals.
    Authors: Zhang Q, Jiang J, Han P, Yuan Q, Zhang J, Zhang X, Xu Y, Cao H, Meng Q, Chen L, Tian T, Wang X, Li P, Hescheler J, Ji G, Ma Y
    Cell Res., 2011;21(4):579-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  76. Efficient derivation of functional floor plate tissue from human embryonic stem cells.
    Authors: Fasano CA, Chambers SM, Lee G, Tomishima MJ, Studer L
    Cell Stem Cell, 2010;6(4):336-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells.
    Authors: Koch S, Capaldo CT, Samarin S, Nava P, Neumaier I, Skerra A, Sacks DB, Parkos CA, Nusrat A
    Mol. Biol. Cell, 2009;20(22):4816-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  78. Dkk-3 is elevated in CSF and plasma of Alzheimer's disease patients.
    Authors: Zenzmaier C, Marksteiner J, Kiefer A, Berger P, Humpel C
    J. Neurochem., 2009;110(2):653-61.
    Species: N/A
    Sample Types: N/A
    Applications: Antibody Absorption
  79. Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells.
    Authors: Cho SW, Yang JY, Sun HJ, Jung JY, Her SJ, Cho HY, Choi HJ, Kim SW, Kim SY, Shin CS
    Bone, 2009;44(6):1069-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  80. Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-beta Signaling.
    Authors: Carthy J, Engstrom U, Heldin C, Moustakas A
    J Cell Biochem, 0;117(4):938-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Dkk-1 Protein
By Anonymous on 07/16/2021
Application: In vitro bioactivity in cell culture
Reason for Rating: Works good for cell assay

Recombinant Human Dkk-1 Protein
By Anonymous on 12/17/2020
Application: ELISA
Reason for Rating: Using the DKK-1 protein as standard for ELISA.

Recombinant Human Dkk-1 Protein
By Anonymous on 10/27/2018
Application: In vitro bioactivity in cell culture
Reason for Rating: Recombinant product worked excellently to block Wnt3a mediated Wnt signaling in breast cancer cells

Recombinant Wnt3a (100 ng/mL) was added to cells containing a Wnt reporter (measured in eGFP) with or without RnD Dkk1 addition at 1 ug/mL


Recombinant Human Dkk-1 Protein
By Anonymous on 12/06/2017
Application: In vitro bioactivity in cell culture

Recombinant Human Dkk-1 Protein
By Anonymous on 08/18/2017
Application: In vitro bioactivity in cell culture