Recombinant Mouse DPPIV/CD26 Protein, CF
Recombinant Mouse DPPIV/CD26 Protein, CF Summary
Ser29-His760, with a C-terminal 9-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in Tris and NaCl.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Assay Buffer: 25 mM Tris, pH 8.0
- Recombinant Mouse DPPIV/CD26 (rmCD26) (Catalog # 954-SE)
- Substrate: Gly-Pro-AMC (Bachem, Catalog # I-1225), 10 mM stock in DMSO.
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmCD26 to 0.2 ng/µL in Assay Buffer.
- Dilute Substrate to 40 µM in Assay Buffer.
- In a plate load 50 µL of 0.2 ng/µL rmCD26 and start the reaction by adding 50 µL of 40 µM Substrate. Include a Substrate blank containing 50 µL Assay Buffer and 50 µL of Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
Specific Activity (pmol/min/µg) =
|Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)|
|amount of enzyme (µg)|
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).
- rmCD26: 0.010 µg
- Substrate: 20 µM
DPPIV/CD26 (EC 184.108.40.206) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.
DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).
- Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
- Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
- Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
- Bernard, A.M. et al. (1994) Biochemistry 33:15204.
- Vivier, I. et al. (1991) J. Immunol. 147:447.
- Callebaut, C. et al. (1993) Science 262:2045.
- Kameoka, J. et al. (1993) Science 261:466.
- Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
- Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
- Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
- Wrenger, S. et al. (2000) FEBS Lett. 466:155.
Citations for Recombinant Mouse DPPIV/CD26 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration
Authors: TH Ambrosi, A Scialdone, A Graja, S Gohlke, AM Jank, C Bocian, L Woelk, H Fan, DW Logan, A Schürmann, LR Saraiva, TJ Schulz
Cell Stem Cell, 2017;20(6):771-784.e6.
Sample Types: Cell Culture Supernates
Elevated hepatic DPP4 activity promotes insulin resistance and non-alcoholic fatty liver disease
Authors: C Baumeier, L Schlüter, S Saussentha, T Laeger, M Rödiger, SA Alaze, L Fritsche, HU Häring, N Stefan, A Fritsche, RW Schwenk, A Schürmann
Mol Metab, 2017;6(10):1254-1263.
Sample Types: Whole Cells
Therapeutic vaccine against DPP4 improves glucose metabolism in mice.
Authors: Pang, Zhengda, Nakagami, Hironori, Osako, Mariana, Koriyama, Hiroshi, Nakagami, Futoshi, Tomioka, Hideki, Shimamura, Munehisa, Kurinami, Hitomi, Takami, Yoichi, Morishita, Ryuichi, Rakugi, Hiromi
Proc Natl Acad Sci U S A, 2014;111(13):E1256-63.
Sample Types: Serum
E3024, 3-but-2-ynyl-5-methyl-2-piperazin-1-yl-3,5-dihydro-4H-imidazo[4,5-d]pyridazin-4-one tosylate, is a novel, selective and competitive dipeptidyl peptidase-IV inhibitor.
Authors: Yasuda N, Nagakura T, Inoue T, Yamazaki K, Katsutani N, Takenaka O, Clark R, Matsuura F, Emori E, Yoshikawa S, Kira K, Ikuta H, Okada T, Saeki T, Asano O, Tanaka I
Eur. J. Pharmacol., 2006;548(1):181-7.
Sample Types: Pharmaceutical
Applications: Enzyme Assay
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