Recombinant Mouse FABP4/A-FABP Protein, CF Summary
Accession # P04117
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MES and NaCl.|
|Reconstitution||Reconstitute at 1 mg/mL in water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.
Fatty acid binding protein-4 (FABP4; also named A- (adipocyte) FABP, ALBP, or adipocyte protein 2 (aP2)) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 5, 6). FABP4 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (Il-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Mouse A- FABP, the product of the FABP4 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta -strands and two alpha ‑helices (4, 8). FABP4 is able to bind long-chain fatty acids, retinoic acids or eicosanoids with one mol of ligand bound per mole of protein (1, 8, 9). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (4, 7, 9). Mouse FABP4 is 92%, 94% and 91% aa identical to human, rat and canine FABP4, respectively. It also shows 26% and 28% aa identity to mouse L-FABP and I‑FABP, respectively.
- Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
- Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
- Zimmerman, A.W. & Veerkamp, J.H. (2007) Protein Sci. 9:2042.
- Bernlohr, D. et al. (1997) Ann. Rev. of Nut. 17:277.
- Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
- Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
- Xu Z. et. al. (1992) Biochemistry. 31:3484.
- Matarese, V. & Bernlohr, D. (1988) J Biol Chem. 263:14544.
- Majava,V. et al. (2010) PLoS One. 5:e10300.
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