Fas ligand (FasL) is a 40 kDa type II membrane protein belonging to the TNF family. In the new TNF super family nomenclature, FasL is referred to as TNFSF6. The specific receptor for FasL is Fas (CD95, Apo-1), a 45 kDa type I transmembrane protein that is a member of the TNF receptor family. FasL is predominantly expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. The Fas/FasL system plays a crucial role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. FasL was reported to be a potent chemoattractant for neutrophils, suggesting a novel proinflammatory function of this molecule. Like other members of the TNF family, the membrane-bound FasL can be cleaved by metalloproteinase to generate the soluble Fas ligand (sFasL) which is mainly a non-covalently linked homotrimer. It has been shown that the membrane-bound TNF‑ alpha and FasL are primary activators of their receptors. In contrast to soluble TNF‑ alpha which has potent cytotoxicity, sFasL is much less cytotoxic. Studies have shown that sFasL may competitively inhibit the killing effect of membrane FasL indicating that the cleaving of membrane FasL might be a mechanism to down‑regulate their activities.
Recombinant Mouse Fas Ligand/TNFSF6 Protein
R&D Systems | Catalog # 526-SA
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Fas Ligand/TNFSF6 Protein (526-SA)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Pro132-Leu279, with an N-terminal Met and 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells.
The ED50 for this effect is 0.25‑1.5 µg/mL in the presence of 10 µg/mL of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
This soluble Recombinant Mouse Fas Ligand/TNFSF6 has weak cytotoxic activity. It has no effects on A20 mouse B cell lymphoma cells which express mouse Fas.
This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the products.
Reviewed Applications
Read 1 review rated 3 using 526-SA in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 526-SA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 526-SA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Fas Ligand/TNFSF6
References
- Suda, T. et al. (1993) Cell 75:1169.
- Kägi, D. et al. (1994) Science 265:528.
- Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
- Seino, K. et al. (1998) J. Immunol. 161:4484.
Alternate Names
Gene Symbol
UniProt
Additional Fas Ligand/TNFSF6 Products
Product Documents for Recombinant Mouse Fas Ligand/TNFSF6 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Fas Ligand/TNFSF6 Protein
For research use only
Citations for Recombinant Mouse Fas Ligand/TNFSF6 Protein
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Application: Apoptosis assayVerified Customer | Posted 11/11/2018rmFas ligand added at 2 ug/mL for 2 day culture.
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FAQs for Recombinant Mouse Fas Ligand/TNFSF6 Protein
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Q: This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
Associated Pathways
IL-2 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-15 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
TNF Superfamily Pathway: Human Ligand-Receptor Interactions & their Associated Functions
