Recombinant Mouse Fas Ligand/TNFSF6 Protein

Newer Version Available: 6128-SA
A New rm FasL is Available! It has ~1000 fold better activity, lower endotoxin specification, and a HA tag!
Formulations:
Catalog # Availability Size / Price Qty
526-SA-050
Product Details
Citations (3)
FAQs
Reviews (1)

Recombinant Mouse Fas Ligand/TNFSF6 Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.25‑1.5 µg/mL in the presence of 10 µg/mL of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). This soluble Recombinant Mouse Fas Ligand/TNFSF6 has weak cytotoxic activity. It has no effects on A20 mouse B cell lymphoma cells which express mouse Fas. This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the products.
Source
Mouse myeloma cell line, NS0-derived mouse Fas Ligand/TNFSF6 protein
Pro132-Leu279, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18 kDa (monomer)
SDS-PAGE
27-33 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

526-SA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

526-SA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fas Ligand/TNFSF6

Fas ligand (FasL) is a 40 kDa type II membrane protein belonging to the TNF family. In the new TNF super family nomenclature, FasL is referred to as TNFSF6. The specific receptor for FasL is Fas (CD95, Apo-1), a 45 kDa type I transmembrane protein that is a member of the TNF receptor family. FasL is predominantly expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. The Fas/FasL system plays a crucial role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. FasL was reported to be a potent chemoattractant for neutrophils, suggesting a novel proinflammatory function of this molecule. Like other members of the TNF family, the membrane-bound FasL can be cleaved by metalloproteinase to generate the soluble Fas ligand (sFasL) which is mainly a non-covalently linked homotrimer. It has been shown that the membrane-bound TNF‑ alpha and FasL are primary activators of their receptors. In contrast to soluble TNF‑ alpha which has potent cytotoxicity, sFasL is much less cytotoxic. Studies have shown that sFasL may competitively inhibit the killing effect of membrane FasL indicating that the cleaving of membrane FasL might be a mechanism to down‑regulate their activities.

References
  1. Suda, T. et al. (1993) Cell 75:1169.
  2. Kägi, D. et al. (1994) Science 265:528.
  3. Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
  4. Seino, K. et al. (1998) J. Immunol. 161:4484.
Entrez Gene IDs
356 (Human); 14103 (Mouse); 25385 (Rat)
Alternate Names
apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6

Citations for Recombinant Mouse Fas Ligand/TNFSF6 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. FasL Modulates Expression of Mmp2 in Osteoblasts
    Authors: E Svandova, B Vesela, H Lesot, J Sadoine, A Poliard, E Matalova
    Front Physiol, 2018;9(0):1314.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
    Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
    J. Immunol., 2007;178(6):3379-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Tissue inhibitor of metalloproteinases-3 facilitates Fas-mediated neuronal cell death following mild ischemia.
    Authors: Wetzel M, Li L, Harms KM, Roitbak T, Ventura PB, Rosenberg GA, Khokha R, Cunningham LA
    Cell Death Differ., 2007;15(1):143-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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Reviews for Recombinant Mouse Fas Ligand/TNFSF6 Protein

Average Rating: 3 (Based on 1 Review)

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Recombinant Mouse Fas Ligand/TNFSF6 Protein
By Anonymous on 11/11/2018
Application: Apoptosis assay
Reason for Rating: Product worked to induce some cytotoxicity at 2 ug/mL, however effect size was far below cytotoxic t cell activity in vitro.

rmFas ligand added at 2 ug/mL for 2 day culture.