Recombinant Mouse Flt-3 Ligand Protein, CF

(42 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.4-2.4 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Gly27-Arg188
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly27
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    Multiple bands between 18-32 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
427-FL/CF
 
427-FL
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 µg/mL in sterile PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL/CF) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.4-2.4 ng/mL.

1 μg/lane of Recombinant Mouse Flt‑3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands between 18-32 kDa. The multiple bands are due to variable glycosylation of the protein.

Background: Flt-3 Ligand

Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature mouse Flt‑3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4-6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. Mouse and human Flt-3 Ligand show cross-species activity (4, 5, 7). Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O‑linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine domain (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3 - 6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). It synergizes with IL-3, GM‑CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). It cooperates with IL‑2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and ‑11 to induce terminal B cell maturation (1, 11). Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation (12).

  • References:
    1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
    2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
    3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
    4. Hannum, C. et al. (1994) Nature 368:643.
    5. Lyman, S.D. et al. (1993) Cell 75:1157.
    6. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
    7. Lyman, S.D. et al. (1994) Blood 83:2795.
    8. McClanahan, T. et al. (1996) Blood 88:3371.
    9. Lyman, S.D. et al. (1995) Oncogene 10:149.
    10. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
    11. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
    12. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.
  • Long Name:
    fms-like Tyrosine Kinase 3 Ligand
  • Entrez Gene IDs:
    2323 (Human); 14256 (Mouse); 493796 (Feline)
  • Alternate Names:
    FL; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease
    Authors: J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin
    Mol Ther Methods Clin Dev, 2017;5(0):241-258.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. T cell progenitor therapy-facilitated thymopoiesis depends upon thymic input and continued thymic microenvironment interaction
    Authors: MJ Smith, DK Reichenbac, SL Parker, MJ Riddle, J Mitchell, KC Osum, M Mohtashami, HE Stefanski, BT Fife, A Bhandoola, KA Hogquist, GA Holländer, JC Zúñiga-Pfl, J Tolar, BR Blazar
    JCI Insight, 2017;2(10):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1
    Authors: S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra
    Nat. Methods, 2017;14(5):531-538.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Alpha 1 Antitrypsin Inhibits Dendritic Cell Activation and Attenuates Nephritis in a Mouse Model of Lupus
    Authors: Ahmed S Elshikha
    PLoS ONE, 2016;11(5):e0156583.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins
    Nat. Med., 2016;22(6):672-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
    Nat Commun, 2016;7(0):13396.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. The non-canonical Wnt receptor Ryk regulates hematopoietic stem cell repopulation in part by controlling proliferation and apoptosis
    Cell Death Dis, 2016;7(11):e2479.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Hepatitis C Virus Stimulates Murine CD8?-Like Dendritic Cells to Produce Type I Interferon in a TRIF-Dependent Manner
    PLoS Pathog, 2016;12(7):e1005736.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis
    PLoS ONE, 2016;11(5):e0154630.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages.
    Authors: Lee S, Park H, Jeon S, Lee C, Seong R, Park S, Hong S
    PLoS ONE, 2015;10(7):e0132329.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Lignin-rich enzyme lignin (LREL), a cellulase-treated lignin-carbohydrate derived from plants, activates myeloid dendritic cells via Toll-like receptor 4 (TLR4).
    J Biol Chem, 2015;290(0):4410-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. FcgammaRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
    Authors: Flores M, Chew C, Tyan K, Huang W, Salem A, Clynes R
    J Immunol, 2015;194(9):4240-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Guanine-modified inhibitory oligonucleotides efficiently impair TLR7- and TLR9-mediated immune responses of human immune cells.
    Authors: Rommler F, Hammel M, Waldhuber A, Muller T, Jurk M, Uhlmann E, Wagner H, Vollmer J, Miethke T
    PLoS ONE, 2015;10(2):e0116703.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  15. Batf3-dependent CD103+ dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice.
    Authors: Martinez-Lopez M, Iborra S, Conde-Garrosa R, Sancho D
    Eur J Immunol, 2015;45(1):119-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Selective Expression of the MAPK Phosphatase Dusp9/MKP-4 in Mouse Plasmacytoid Dendritic Cells and Regulation of IFN-beta Production.
    Authors: Niedzielska M, Raffi F, Tel J, Muench S, Jozefowski K, Alati N, Lahl K, Mages J, Billmeier U, Schiemann M, Appelt U, Wirtz S, Sparwasser T, Hochrein H, Figdor C, Keyse S, Lang R
    J Immunol, 2015;195(4):1753-62.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  17. Sphingosine kinase 2 promotes acute lymphoblastic leukemia by enhancing MYC expression.
    Authors: Wallington-Beddoe C, Powell J, Tong D, Pitson S, Bradstock K, Bendall L
    Cancer Res, 2014;74(10):2803-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Ectopic TLX1 expression accelerates malignancies in mice deficient in DNA-PK.
    Authors: Krutikov K, Zheng Y, Chesney A, Huang X, Vaags A, Evdokimova V, Hough M, Chen E
    PLoS ONE, 2014;9(2):e89649.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Leukotriene B4 amplifies eosinophil accumulation in response to nematodes.
    Authors: Patnode M, Bando J, Krummel M, Locksley R, Rosen S
    J Exp Med, 2014;211(7):1281-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014;124(9):1393-403.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. The TCR ligand-inducible expression of CD73 marks gammadelta lineage commitment and a metastable intermediate in effector specification.
    Authors: Coffey F, Lee S, Buus T, Lauritsen J, Wong G, Joachims M, Thompson L, Zuniga-Pflucker J, Kappes D, Wiest D
    J Exp Med, 2014;211(2):329-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential.
    Authors: Treanor L, Zhou S, Janke L, Churchman M, Ma Z, Lu T, Chen S, Mullighan C, Sorrentino B
    J Exp Med, 2014;211(4):701-13.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Modified vaccinia virus Ankara triggers type I IFN production in murine conventional dendritic cells via a cGAS/STING-mediated cytosolic DNA-sensing pathway.
    Authors: Dai P, Wang W, Cao H, Avogadri F, Dai L, Drexler I, Joyce J, Li X, Chen Z, Merghoub T, Shuman S, Deng L
    PLoS Pathog, 2014;10(4):e1003989.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Dendritic cells activated by double-stranded RNA induce arthritis via autocrine type I IFN signaling.
    Authors: Narendra S, Chalise J, Hook N, Magnusson M
    J Leukoc Biol, 2014;95(4):661-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. Induction of a hemogenic program in mouse fibroblasts.
    Authors: Pereira C, Chang B, Qiu J, Niu X, Papatsenko D, Hendry C, Clark N, Nomura-Kitabayashi A, Kovacic J, Ma'ayan A, Schaniel C, Lemischka I, Moore K
    Cell Stem Cell, 2013;13(2):205-18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. Gata2 cis-element is required for hematopoietic stem cell generation in the mammalian embryo.
    Authors: Gao, Xin, Johnson, Kirby D, Chang, Yuan-I, Boyer, Meghan E, Dewey, Colin N, Zhang, Jing, Bresnick, Emery H
    J Exp Med, 2013;210(13):2833-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  27. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor.
    Authors: Smith, Christop, Berry, Donna M, McGlade, C Jane
    J Cell Sci, 2013;126(0):1366-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  29. Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
    Authors: Manley, Nathan C, Caso, Javier R, Works, Melissa, Cutler, Andrew B, Zemlyak, Ilona, Sun, Guohua, Munhoz, Carolina, Chang, Sydney, Sorrells, Shawn F, Ermini, Florian, Decker, Johannes, Bertrand, Anthony, Dinkel, Klaus M, Steinberg, Gary K, Sapolsky, Robert M
    PLoS ONE, 2013;8(4):e61789.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  30. The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells.
    Authors: Gatto D, Wood K, Caminschi I, Murphy-Durland D, Schofield P, Christ D, Karupiah G, Brink R
    Nat Immunol, 2013;14(5):446-53.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  31. Synergistic induction of interferon alpha through TLR-3 and TLR-9 agonists identifies CD21 as interferon alpha receptor for the B cell response.
    Authors: Kim D, Niewiesk S
    PLoS Pathog, 2013;9(3):e1003233.
    Species: Cotton Rat
    Sample Type: Whole Cells
    Application: Bioassay
  32. The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice.
    Authors: Iborra S, Izquierdo HM, Martinez-Lopez M, Blanco-Menendez N, Reis E Sousa C, Sancho D
    J. Clin. Invest., 2012;122(5):1628-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  33. Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin.
    Authors: Shinohara ML, Kim JH, Garcia VA, Cantor H
    Immunity, 2008;29(1):68-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  34. IL-23 and the Th17 pathway promote inflammation and impair antifungal immune resistance.
    Authors: Zelante T, De Luca A, Bonifazi P, Montagnoli C, Bozza S, Moretti S, Belladonna ML, Vacca C, Conte C, Mosci P, Bistoni F, Puccetti P, Kastelein RA, Kopf M, Romani L
    Eur. J. Immunol., 2007;37(10):2695-706.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  35. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh&lt;/LastName&gt;&lt;ForeNam D&lt;/Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. Jagged2-expressing hematopoietic progenitors promote regulatory T cell expansion in the periphery through notch signaling.
    Authors: Kared H, Adle-Biassette H, Fois E, Masson A, Bach JF, Chatenoud L, Schneider E, Zavala F
    Immunity, 2006;25(5):823-34.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  37. Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo.
    Authors: Braun BS, Archard JA, Van Ziffle JA, Tuveson DA, Jacks TE, Shannon K
    Blood, 2006;108(6):2041-4.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  38. Comparative analysis of the fate of donor dendritic cells and B cells and their influence on alloreactive T cell responses under tacrolimus immunosuppression.
    Authors: Azhipa O, Kimizuka K, Nakao A, Toyokawa H, Okuda T, Neto JS, Alber SM, Kaizu T, Thomson AW, Demetris AJ, Murase N
    Clin. Immunol., 2005;114(2):199-209.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  39. T cell repertoire scanning is promoted by dynamic dendritic cell behavior and random T cell motility in the lymph node.
    Authors: Miller MJ, Hejazi AS, Wei SH, Cahalan MD, Parker I
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(4):998-1003.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  40. Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody.
    Authors: Asselin-Paturel C, Brizard G, Pin JJ, Briere F, Trinchieri G
    J. Immunol., 2003;171(12):6466-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  41. Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines.
    Authors: Blumenthal RD, Reising A, Leon E
    Clin. Cancer Res., 2002;8(5):1301-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  42. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000;95(6):1957-66.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA development
Expand to show all 42 Citations

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