Recombinant Mouse Glypican 6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9429-GP-050

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Recombinant Mouse Glypican 6 Fc Chimera Protein Bioactivity
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Recombinant Mouse Glypican 6 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Glypican 6 Fc Chimera is immobilized at 3 μg/mL, 100 μL/well, the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB) that produces 50% of the optimal binding response is 2.5-15 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Glypican 6 protein
Mouse Glypican 6
(Asp24-Val513)
Accession # Q9R087
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp24 & Ser355
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
83 kDa (Asp24-Val513/mFc), 44 kDa (Ser355-Val513/mFc) and 38 kDa (Asp24-Arg354)
SDS-PAGE
84-98 kDa (Asp24-Val513/mFc), 53-68 kDa (Asp24-Val513/mFc) and 33-40 kDa (Asp24-Arg354)

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9429-GP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9429-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Glypican 6 Fc Chimera Protein Bioactivity View Larger

When Recombinant Mouse Glypican 6 Fc Chimera (Catalog # 9429-GP) is immobilized at 3 µg/mL, 100 µL/well, Recombinant Human FGF basic 146 aa (Catalog # 233-FB) binds with an ED50 of 2.5-15 ng/mL.

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Background: Glypican 6

The Glypicans (Glypiated Proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60-70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Mouse Glypican 6 (GPC-6) is synthesized as a 555 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 507 aa mature region and a 25 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan. Mouse to human, there is 96% aa identity over the entire GPC-6 molecule (5). Glypican proteins are located in the extracellular matrix of cells and are important for tissue organization, cell proliferation, adhesion, migration and differentiation when they interact with extracellular or cytoplasmic ligands (7, 8). Glypicans act as co‑receptors to enhance the activity of heparin-binding factors such as fibroblast growth factors and Wnt. Due to their ability to affect growth factor-mediated signaling, glypicans are known to have both pro- and anti-tumorigenic effects. GPC-6 overexpression has been associated with metastatic breast cancer, gastric cancer, and is known to regulate cardiomyocyte growth through the ERK1/2 signalling pathway where up-regulation is known to play a role in heart failure (9-11).

References
  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
  2. Filmus, J. (2001) Glycobiology 11:19R.
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
  4. Filmus, J. (2003) Glycoconj. J. 19:319.
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
  7. Kirn-Safran, C. et al. (2009) Cell. Mol. Life Sci. 66:3421.
  8. Syrokou, A. et al. (1999) Cell Prolif. 32:85.
  9. Dinccelik-Aslan, M. et al. (2015) Mol. Clin. Oncol. 3:584.
  10. Yiu, G.K. et al. (2011) Biochem. J. 440:157.
  11. Melleby, A.O. et al. (2016) PLoS One. 11:e0165079.
Entrez Gene IDs
10082 (Human); 23888 (Mouse)
Alternate Names
Glypican 6; glypican proteoglycan 6; glypican-6; GPC6; MGC126288; OMIMD1

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