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Recombinant Mouse GM-CSF Protein

R&D Systems | Catalog # 415-ML

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse GM-CSF Protein (415-ML)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q14AD9

Applications

Bioactivity
View all GM-CSF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse GM-CSF protein
Ala18-Lys141, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

14.3 kDa

Activity

Measured in a cell proliferation assay using DA3 mouse myeloma cells. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95.
The ED50 for this effect is 5‑30 pg/mL.

Reviewed Applications

Read 15 reviews rated 4.6 using 415-ML in the following applications:

Scientific Data Images for Recombinant Mouse GM-CSF Protein

Recombinant Mouse GM‑CSF Protein SEC-MALS.

Recombinant Mouse GM-CSF Protein (Catalog # 415-ML) has a molecular weight (MW) of 14.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Mouse GM-CSF Protein Bioactivity

Recombinant Mouse GM-CSF Protein Bioactivity

Recombinant Mouse GM-CSF (Catalog # 415-ML) stimulates cell proliferation of the DA3 mouse myeloma cell line. The ED50 for this effect is 5-30 pg/mL.
Recombinant Mouse GM-CSF Protein SDS-PAGE

Recombinant Mouse GM-CSF Protein SDS-PAGE

1 μg/lane of Recombinant Mouse GM-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 415-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 415-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49%-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM‑CSF is fully active on mouse cells (14, 15).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779.
  10. Gough, N.M. et al. (1984) Nature 309:763.
  11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
  15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Gene Symbol

CSF2

UniProt

Q14AD9

Additional GM-CSF Products

Product Documents for Recombinant Mouse GM-CSF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 415-ML (with carrier)
Download SDS 415-ML/CF (carrier free)

Product Specific Notices for Recombinant Mouse GM-CSF Protein

For research use only

Citations for Recombinant Mouse GM-CSF Protein

Customer Reviews for Recombinant Mouse GM-CSF Protein (15)

4.6 out of 5
15 Customer Ratings
5 Stars
67%
4 Stars
27%
3 Stars
7%
2 Stars
0%
1 Stars
0%

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  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 07/11/2025
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 04/05/2024
    Check tumor suppression
    Recombinant Mouse GM-CSF Protein 415-ML
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/13/2021
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Cell differentiation
    Verified Customer | Posted 09/26/2021
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/23/2021
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/26/2021
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 02/17/2020
    Recombinant Mouse GM-CSF Protein 415-ML
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: ELISA positive control
    Verified Customer | Posted 09/16/2019
    Recombinant Mouse GM-CSF Protein 415-ML
  • Name: Anonymous
    Application: Dendritic cell differentiation
    Verified Customer | Posted 11/02/2018
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 07/13/2018
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Characterization prior to SEC
    Verified Customer | Posted 02/12/2018
    Recombinant Mouse GM-CSF Protein 415-ML
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 08/03/2016
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 05/16/2016
  • Recombinant Mouse GM-CSF Protein
    Name: Tima Thomas
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016
  • Recombinant Mouse GM-CSF Protein
    Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 01/14/2016

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