Recombinant Mouse IFN-beta Protein
Recombinant Mouse IFN-beta Protein Summary
Product Specifications
The specific activity of recombinant Mouse IFN‑ beta is approximately 1.2 x 109 IU/mg, which is calibrated against Murine IFN‑ beta WHO International Standard. The Murine IFN‑ beta WHO International Standard (NR-3079) was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH.
Ile22-Asn182
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8234-MB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80 with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8234-MB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images
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Recombinant Mouse IFN-beta (Catalog # 8234-MB) suppresses viral activity using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is 1-6 pg/mL.
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1 μg/lane of Recombinant Mouse IFN-beta (Catalog # 8234-MB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 32.7 and 32.9 kDa, respectively.
Reconstitution Calculator
Background: IFN-beta
Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).
- González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
- Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
- Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
- Häcker, H. et al. (2006) Nature 439:204.
- Teige, I. et al. (2003) J. Immunol. 170:4776.
- Shinohara, M.L. et al. (2008) Immunity 29:68.
- Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
- Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
- Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
- Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
- Wang, H. et al. (2011) J. Immunol 186:675.
Citations for Recombinant Mouse IFN-beta Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The Innate Immune Response to Herpes Simplex Virus 1 Infection Is Dampened in the Newborn Brain and Can Be Modulated by Exogenous Interferon Beta To Improve Survival
Authors: D Giraldo, DR Wilcox, R Longnecker
MBio, 2020;11(3):.
Species: Mouse
Sample Types:
Applications: In Vivo -
Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS
Authors: S Hosseini, K Michaelsen, G Grigoryan, C Chhatbar, U Kalinke, M Korte
Cell Rep, 2020;31(7):107666.
Species: Mouse
Sample Types: Whole Cells
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Repression of the Type I Interferon pathway underlies MYC & KRAS-dependent evasion of NK & B cells in Pancreatic Ductal Adenocarcinoma
Authors: N Muthalagu, T Monteverde, X Raffo-Irao, R Wiesheu, D Whyte, A Hedley, S Laing, B Kruspig, R Upstill-Go, R Shaw, S Neidler, C Rink, SA Karim, K Gyuraszova, C Nixon, W Clark, AV Biankin, LM Carlin, SB Coffelt, OJ Sansom, JP Morton, DJ Murphy
Cancer Discov, 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Manganese is critical for antitumor immune responses via cGAS-STING and improves the efficacy of clinical immunotherapy
Authors: M Lv, M Chen, R Zhang, W Zhang, C Wang, Y Zhang, X Wei, Y Guan, J Liu, K Feng, M Jing, X Wang, YC Liu, Q Mei, W Han, Z Jiang
Cell Res., 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget's disease
Authors: SW Wong, BW Huang, X Hu, E Ho Kim, JP Kolb, RJ Padilla, P Xue, L Wang, TH Oguin, PA Miguez, HC Tseng, CC Ko, J Martinez
Cell Death Differ., 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Genetic Screen in Chlamydia muridarum Reveals Role for an Interferon-Induced Host Cell Death Program in Antimicrobial Inclusion Rupture
Authors: AM Giebel, S Hu, K Rajaram, R Finethy, E Toh, JA Brothwell, SG Morrison, RJ Suchland, BD Stein, J Coers, RP Morrison, DE Nelson
MBio, 2019;10(2):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations
Authors: A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston
Nat Commun, 2018;9(1):2229.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-? and Cholesterol 25-hydroxylase in Jejunum
Authors: P Mukherjee, G Hough, A Chattopadh, M Navab, HR Fogelman, D Meriwether, K Williams, S Bensinger, T Moller, KF Faull, AJ Lusis, L Iruela-Ari, KI Bostrom, P Tontonoz, ST Reddy, AM Fogelman
J. Lipid Res., 2017;0(0):.
Applications: ELISA (Standard) -
Reestablishment of p53/Arf and interferon-? pathways mediated by a novel adenoviral vector potentiates antiviral response and immunogenic cell death
Authors: A Hunger, RF Medrano, DB Zanatta, PR Del Valle, CA Merkel, TA Salles, DG Ferrari, TK Furuya, SO Bustos, R de Freitas, E Costanzi-S, BE Strauss
Cell Death Discov, 2017;3(0):17017.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The kinase TBK1 functions in dendritic cells to regulate T cell homeostasis, autoimmunity, and antitumor immunity
Authors: Y Xiao, Q Zou, X Xie, T Liu, HS Li, Z Jie, J Jin, H Hu, G Manyam, L Zhang, X Cheng, H Wang, I Marie, DE Levy, SS Watowich, SC Sun
J. Exp. Med., 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Runx1 Orchestrates Sphingolipid Metabolism and Glucocorticoid Resistance in Lymphomagenesis
Authors: J C Neil
J. Cell. Biochem., 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
SARS-CoV-2 Receptor ACE2 Is an Interferon-Stimulated Gene in Human Airway Epithelial Cells and Is Detected in Specific Cell Subsets across Tissues.
Authors: Ziegler C, Allon S, Nyquist S, Mbano I, Miao V, Tzouanas C, Cao Y, Yousif A, Bals J, Hauser B, Feldman J, Muus C, Wadsworth M, Kazer S, Hughes T, Doran B, Gatter G, Vukovic M, Taliaferro F, Mead B, Guo Z, Wang J, Gras D, Plaisant M, Ansari M, Angelidis I, Adler H, Sucre J, Taylor C, Lin B, Waghray A, Mitsialis V, Dwyer D, Buchheit K, Boyce J, Barrett N, Laidlaw T, Carroll S, Colonna L, Tkachev V, Peterson C, Yu A, Zheng H, Gideon H, Winchell C, Lin P, Bingle C, Snapper S, Kropski J, Theis F, Schiller H, Zaragosi L, Barbry P, Leslie A, Kiem H, Flynn J, Fortune S, Berger B, Finberg R, Kean L, Garber M, Schmidt A, Lingwood D, Shalek A, Ordovas-Montanes J
Cell, 0;181(5):1016-1035.e19.
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