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Recombinant Mouse IFN-beta Protein

R&D Systems | Catalog # 8234-MB

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Mouse IFN-beta Protein (8234-MB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P01575

Structure / Form

Monomer

Applications

Bioactivity
View all IFN-beta Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-beta protein
Ile22-Asn182

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile22

Predicted Molecular Mass

20 kDa

SDS-PAGE

30-38 kDa, reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 1.00-6.00 pg/mL.

Reviewed Applications

Read 1 review rated 4 using 8234-MB in the following applications:

Scientific Data Images for Recombinant Mouse IFN-beta Protein

Recombinant Mouse IFN-beta Protein Bioactivity

Recombinant Mouse IFN-beta Protein Bioactivity

Recombinant Mouse IFN-beta (Catalog # 8234-MB) suppresses viral activity using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is 1.00-6.00 pg/mL.
Recombinant Mouse IFN-beta Protein SDS-PAGE

Recombinant Mouse IFN-beta Protein SDS-PAGE

1 μg/lane of Recombinant Mouse IFN-beta (Catalog # 8234-MB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 32.7 and 32.9 kDa, respectively.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8234-MB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 8234-MB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

References

  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Teige, I. et al. (2003) J. Immunol. 170:4776.
  6. Shinohara, M.L. et al. (2008) Immunity 29:68.
  7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
  11. Wang, H. et al. (2011) J. Immunol 186:675.

Long Name

Interferon beta

Alternate Names

IFB, IFF, IFNB, IFNB1, IFNbeta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Gene Symbol

IFNB1

UniProt

P01575

Additional IFN-beta Products

Product Documents for Recombinant Mouse IFN-beta Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 8234-MB (with carrier)
Download SDS 8234-MB/CF (carrier free)

Product Specific Notices for Recombinant Mouse IFN-beta Protein

For research use only

Citations for Recombinant Mouse IFN-beta Protein

Customer Reviews for Recombinant Mouse IFN-beta Protein (1)

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FAQs for Recombinant Mouse IFN-beta Protein

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  • Q: What are the differences between Recombinant Mouse IFN-beta Protein (Catalog # 8234-MB) and Recombinant Mouse IFN-beta Protein (Catalog # 12400-1)?

    A: Catalog # 12400-1 is raised in E. coli and has an endotoxin specification of <1 EU/ug. Catalog # 8234-MB is raised in HEK293 cells, and it has a lower endotoxin specification of <0.10 EU per 1 ug of protein.

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