Recombinant Mouse IL-1 alpha/IL-1F1 Protein

Formulations:
Catalog # Availability Size / Price Qty
400-ML-005
400-ML-025
Recombinant Mouse IL-1 alpha/IL-1F1 Protein Bioactivity
2 Images
Product Details
Citations (16)
FAQs
Reviews (1)

Recombinant Mouse IL-1 alpha/IL-1F1 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 3-7 pg/mL.
Source
E. coli-derived mouse IL-1 alpha/IL-1F1 protein
Ser6-Ser161
Accession #
N-terminal Sequence
Analysis
Ser6
Predicted Molecular Mass
18 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

400-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

400-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Mouse IL-1 alpha/IL-1F1 Protein Bioactivity View Larger

Recombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 3-7 pg/mL.

SDS-PAGE Recombinant Mouse IL-1 alpha/IL-1F1 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse IL‑1 alpha /IL‑1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 alpha/IL-1F1

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names
Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha

Citations for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction
    Authors: PC Duncker, JS Stoolman, AK Huber, BM Segal
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Secreted IL-1alpha promotes T-cell activation and expansion of CD11b(+) Gr1(+) cells in carbon tetrachloride-induced liver injury in mice.
    Authors: Lin D, Lei L, Zhang Y, Hu B, Bao G, Liu Y, Song Y, Liu C, Wu Y, Zhao L, Yu X, Liu H
    Eur J Immunol, 2015;45(7):2084-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor kappaB (RANK) Cooperate to Induce Osteoclastogenesis.
    Authors: Jules J, Wang S, Shi Z, Liu J, Wei S, Feng X
    J Biol Chem, 2015;290(39):23738-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. LZTFL1 Upregulated by All-Trans Retinoic Acid during CD4+ T Cell Activation Enhances IL-5 Production.
    Authors: Jiang H, Promchan K, Lin B, Lockett S, Chen D, Marshall H, Badralmaa Y, Natarajan V
    J Immunol, 2015;196(3):1081-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M.
    Authors: Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F
    PLoS ONE, 2014;9(7):e101937.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Distinctive subdomains in the resorbing surface of osteoclasts.
    Authors: Szewczyk K, Fuller K, Chambers T
    PLoS ONE, 2013;8(3):e60285.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. IL-1beta promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation.
    Authors: Jayaraman P, Sada-Ovalle I, Nishimura T, Anderson A, Kuchroo V, Remold H, Behar S
    J Immunol, 2013;190(8):4196-204.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
    Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
    J. Immunol., 2010;184(0):5263-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cells.
    Authors: Dienz O, Eaton SM, Bond JP, Neveu W, Moquin D, Noubade R, Briso EM, Charland C, Leonard WJ, Ciliberto G, Teuscher C, Haynes L, Rincon M
    J. Exp. Med., 2009;206(1):69-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Protection of CD8+ T cells from activation-induced cell death by IL-18.
    Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
    J. Leukoc. Biol., 2007;82(1):142-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex.
    Authors: Chackerian AA, Oldham ER, Murphy EE, Schmitz J, Pflanz S, Kastelein RA
    J. Immunol., 2007;179(4):2551-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. IL-1 mediates TNF-induced osteoclastogenesis.
    Authors: Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL
    J. Clin. Invest., 2005;115(2):282-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Loss of transforming growth factor counteraction on interleukin 1 mediated effects in cartilage of old mice.
    Authors: Scharstuhl A, van Beuningen HM, Vitters EL, van der Kraan PM, van den Berg WB
    Ann. Rheum. Dis., 2002;61(12):1095-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. CD40 regulates the processing of NF-kappaB2 p100 to p52.
    Authors: Coope HJ, Atkinson PG, Huhse B, Belich M, Janzen J, Holman MJ, Klaus GG, Johnston LH, Ley SC
    EMBO J., 2002;21(20):5375-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse IL-1 alpha/IL-1F1 Protein
By Anonymous on 09/24/2018
Application: Cell migration/motility