Recombinant Mouse IL-11 Protein

Formulations:
Catalog # Availability Size / Price Qty
418-ML-005
418-ML-025
Product Details
Citations (25)
FAQs
Reviews (3)

Recombinant Mouse IL-11 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.04-0.4 ng/mL.
Source
E. coli-derived mouse IL-11 protein
Gly23-Leu199, with an N-terminal Met and Pro25-Leu199
Accession #
N-terminal Sequence
Analysis
Met & Pro25
Predicted Molecular Mass
19 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

418-ML

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

418-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water. 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-11

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-4). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The mouse IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature mouse IL-11 shares 88%, 97%, and 89% aa sequence identity with human, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (5). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (6-8).

References
  1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
  3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
  4. Morris, J.C. et al. (1996) Exp. Hematol. 24:1369.
  5. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
  6. Curtis, D.J. et al. (1997) Blood 90:4403.
  7. Baumann, H. et al. (1996) J. Immunol. 157:284.
  8. Karow, J. et al. (1996) Biochem. J. 318:489.
Long Name
Interleukin 11
Entrez Gene IDs
3589 (Human); 16156 (Mouse); 171040 (Rat)
Alternate Names
Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin

Citations for Recombinant Mouse IL-11 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis
    Authors: X Zhang, N Kiapour, S Kapoor, T Khan, M Thamilaras, Y Tao, S Cohen, R Miller, RA Sobel, S Markovic-P
    J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation
    Authors: MJ Delás, BT Jackson, T Kovacevic, S Vangelisti, E Munera Mar, SA Wild, EM Stork, N Erard, SRV Knott, GJ Hannon
    Cell Rep, 2019;27(3):719-729.e6.
    Species: Mouse
    Sample Types: cell culture
    Applications: Cell Culture
  3. A single cell hematopoietic landscape resolves eight lineage trajectories and defects in Kit mutant mice
    Authors: JS Dahlin, FK Hamey, B Pijuan-Sal, M Shepherd, WWY Lau, S Nestorowa, C Weinreb, S Wolock, R Hannah, E Diamanti, DG Kent, B Göttgens, NK Wilson
    Blood, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Hematopoietic Stem Cells as a Novel Source of Dental Tissue Cells
    Authors: KR Wilson, IH Kang, U Baliga, Y Xiong, S Chatterjee, E Moore, B Parthiban, K Thyagaraja, JL Borke, S Mehrotra, KL Kirkwood, AC LaRue, M Ogawa, M Mehrotra
    Sci Rep, 2018;8(1):8026.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Authors: JE Draper, P Sroczynska, MZH Fadlullah, R Patel, G Newton, W Breitwiese, V Kouskoff, G Lacaud
    PLoS Genet., 2018;14(1):e1007127.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway
    Authors: S Ollila, E Domènech-M, K Laajanen, IP Wong, S Tripathi, N Pentinmikk, Y Gao, Y Yan, EH Niemelä, TC Wang, B Viollet, G Leone, P Katajisto, K Vaahtomeri, TP Mäkelä
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  7. Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1
    Authors: S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra
    Nat. Methods, 2017;14(5):531-538.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis
    PLoS ONE, 2016;11(5):e0154630.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Loss of Sfrp2 in the Niche Amplifies Stress-Induced Cellular Responses, and Impairs the in Vivo Regeneration of the Hematopoietic Stem Cell Pool
    Authors: Franziska Ruf
    Stem Cells, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor.
    Authors: Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K
    Stem Cells, 2015;33(3):976-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. A Key Role for Leukemia Inhibitory Factor in C26 Cancer Cachexia.
    Authors: Seto D, Kandarian S, Jackman R
    J Biol Chem, 2015;290(32):19976-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Asymmetry in skeletal distribution of mouse hematopoietic stem cell clones and their equilibration by mobilizing cytokines.
    Authors: Verovskaya, Evgenia, Broekhuis, Mathilde, Zwart, Erik, Weersing, Ellen, Ritsema, Martha, Bosman, Lisette, van Poele, Theo, de Haan, Gerald, Bystrykh, Leonid V
    J Exp Med, 2014;211(3):487-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Optimal attenuation of experimental autoimmune encephalomyelitis by intravenous immunoglobulin requires an intact interleukin-11 receptor.
    Authors: Figueiredo C, Drohomyrecky P, McCarthy S, Leontyev D, Ma X, Branch D, Dunn S
    PLoS ONE, 2014;9(7):e101947.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  14. Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq.
    Authors: Elsaeidi, Fairouz, Bemben, Michael, Zhao, Xiao-Fen, Goldman, Daniel
    J Neurosci, 2014;34(7):2632-44.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Bioassay
  15. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B.
    Authors: Carnevalli L, Scognamiglio R, Cabezas-Wallscheid N, Rahmig S, Laurenti E, Masuda K, Jockel L, Kuck A, Sujer S, Polykratis A, Erlacher M, Pasparakis M, Essers M, Trumpp A
    J Exp Med, 2014;211(5):769-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by blockade of interleukin-11.
    Authors: Mitchell HM, White DM, Domowicz MS, Kraig RP
    J. Neurochem., 2011;117(2):187-96.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  19. Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.
    Authors: Cook BD, Liu S, Evans T
    Blood, 2011;117(24):6489-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. A role for IL-27p28 as an antagonist of gp130-mediated signaling.
    Authors: Stumhofer JS, Tait ED, Quinn WJ, Hosken N, Spudy B, Goenka R, Fielding CA, O'Hara AC, Chen Y, Jones ML, Saris CJ, Rose-John S, Cua DJ, Jones SA, Elloso MM, Grotzinger J, Cancro MP, Levin SD, Hunter CA
    Nat. Immunol., 2010;11(12):1119-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
    Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
    Cell. Signal., 2009;21(1):151-60.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Interleukin-11 potentiates oligodendrocyte survival and maturation, and myelin formation.
    Authors: Zhang Y, Taveggia C, Melendez-Vasquez C, Einheber S, Raine CS, Salzer JL, Brosnan CF, John GR
    J. Neurosci., 2006;26(47):12174-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo.
    Authors: Braun BS, Archard JA, Van Ziffle JA, Tuveson DA, Jacks TE, Shannon K
    Blood, 2006;108(6):2041-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse IL-11 Protein

Average Rating: 4 (Based on 3 Reviews)

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Recombinant Mouse IL-11 Protein
By Anonymous on 05/26/2020
Application: Size exclusion chromatography
Reason for Rating: 65% monomer by SEC.

Recombinant Mouse IL-11 Protein
By Anonymous on 02/01/2017
Application: In vitro bioactivity in cell culture

Recombinant Mouse IL-11 Protein
By Anonymous on 08/03/2016
Application: Stem/Immune cell maintenance or differentiation