Recombinant Mouse IL-11 Protein

R&D Systems | Catalog # 418-ML

R&D Systems
Discontinued Product
418-ML has been discontinued. View all IL-11 products.

Key Product Details

Source

E. coli

Accession Number

P47873

Applications

Bioactivity
View all IL-11 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse IL-11 protein
Gly23-Leu199, with an N-terminal Met and Pro25-Leu199

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Pro25

Predicted Molecular Mass

19 kDa

Activity

Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813.
The ED50 for this effect is 0.04-0.4 ng/mL.

Reviewed Applications

Read 3 reviews rated 4 using 418-ML in the following applications:

Formulation, Preparation, and Storage

418-ML
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-11

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-4). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The mouse IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature mouse IL-11 shares 88%, 97%, and 89% aa sequence identity with human, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (5). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (6-8).

References

  1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
  3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
  4. Morris, J.C. et al. (1996) Exp. Hematol. 24:1369.
  5. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
  6. Curtis, D.J. et al. (1997) Blood 90:4403.
  7. Baumann, H. et al. (1996) J. Immunol. 157:284.
  8. Karow, J. et al. (1996) Biochem. J. 318:489.

Long Name

Interleukin 11

Alternate Names

AGIF, IL11, Oprelvekin

Entrez Gene IDs

3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)

Gene Symbol

IL11

UniProt

P47873

Additional IL-11 Products

Product Documents for Recombinant Mouse IL-11 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IL-11 Protein

For research use only

Citations for Recombinant Mouse IL-11 Protein

Customer Reviews for Recombinant Mouse IL-11 Protein (3)

4 out of 5
3 Customer Ratings
5 Stars
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  • Recombinant Mouse IL-11 Protein
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    Application: Size exclusion chromatography
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    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 02/01/2017
  • Recombinant Mouse IL-11 Protein
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    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 08/03/2016

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