Recombinant Mouse IL-15R alpha Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
551-MR-100
Product Details
Citations (32)
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Recombinant Mouse IL-15R alpha Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. Ruchatz, H. et al. (1998) J. Immunol. 160:5654. The ED50 for this effect is 0.1-0.5 ng/mL in the presence of 0.1 ng/mL recombinant human IL-15.
Source
Mouse myeloma cell line, NS0-derived mouse IL-15 R alpha protein
Mouse IL-15 R alpha
(Gly33 - Lys205)
Accession # Q60819
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Contains a small amount (10%) of free IL-15 R alpha and Fc generated by proteolytic cleavage.
Accession #
N-terminal Sequence
Analysis
Gly33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44.9 kDa (monomer)
SDS-PAGE
80-90 kDa, 42 kDa and 35 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

551-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-15R alpha

Interleukin 15 Receptor alpha (IL 15 R alpha ), also known as CD215, is a widely expressed 60 kDa transmembrane glycoprotein that plays an important role in the homeostasis and activation of NK cells and CD8+ memory T cells and participates in the development and function of many other hematopoietic cell types and non‑immune cell types (1 ‑ 3). Mature mouse IL‑15 R alpha consists of a 173 aa extracellular domain (ECD) containing one N‑linked glycosylation site, a 21 aa transmembrane segment, and a 37 aa cytoplasmic tail (4). Within the ECD, mouse IL‑15 R alpha shares 59% and 89% aa sequence identity with human and rat IL‑15 R alpha, respectively. Alternate splicing of mouse IL‑15 R alpha generates additional isoforms with an N‑terminal truncation or variable length deletions in the ECD. IL‑15 R alpha binds to Interleukin‑15 with high affinity (4). IL‑15 additionally interacts with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL‑2 receptor complex (5, 6). The use of shared receptor components contributes to the overlapping biological effects of IL‑15 and IL‑2. The dominant mechanism of IL‑15 action is known as transpresentation in which IL‑15/IL‑15 R alpha complexes are expressed on the surface of one cell and interact with complexes of IL‑2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL‑15 even if they do not express IL‑15 R alpha (8 ‑ 10). IL‑15/IL‑15 R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL‑15/IL‑15 R alpha expressing cells (11, 12). Shed soluble forms of IL‑15 R alpha retain the ability to bind tightly to IL‑15 and can inhibit IL‑15 bioactivity (4, 13, 14).

References
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  3. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  4. Giri, J.G. et al. (1995) EMBO 14:3654.
  5. Grabstein, K. et al. (1994) Science 264:965.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  8. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  9. Dubois, S. et al. (2002) Immunity 17:537.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  13. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  14. Mortier, E. et al. (2004) J. Immunol. 173:1681.
Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

Citations for Recombinant Mouse IL-15R alpha Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Design and characterisation of a novel interleukin-15 receptor alpha fusion protein and analysis of interleukin-15 complexation
    Authors: AS Schmid, D Neri
    PLoS ONE, 2019;14(7):e0219313.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Size Exclusion Chromatography
  2. NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity
    Authors: N Simonovi?, A Witalisz-S, K Meissl, C Lassnig, U Reichart, T Kolbe, M Farlik, C Bock, V Sexl, M Müller, B Strobl
    J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Interleukin-15 Complex Treatment Protects Mice from Cerebral Malaria by Inducing Interleukin-10-Producing Natural Killer Cells
    Authors: KS Burrack, MA Huggins, E Taras, P Dougherty, CM Henzler, R Yang, S Alter, EK Jeng, HC Wong, M Felices, F Cichocki, JS Miller, GT Hart, AJ Johnson, SC Jameson, SE Hamilton
    Immunity, 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1
    Authors: JH DeLong, AO Hall, C Konradt, GM Coppock, J Park, G Harms Prit, CA Hunter
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Frequencies of IL-15R?+ cells in patients with Beh�et's disease and the effects of overexpressing IL-15R?+ on disease symptoms in mice
    Authors: SMS Islam, B Choi, J Choi, ES Lee, S Sohn
    Cytokine, 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. NKG2D stimulation of CD8+ T cells during priming promotes their capacity to produce cytokines in response to viral infection in mice
    Authors: I Kavazovi?, M Lenarti?, V Jelen?i?, S Jurkovi?, NA Lemmermann, S Jonji?, B Poli?, FM Wensveen
    Eur. J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. IL-15 Complexes Induce Migration of Resting Memory CD8 T Cells into Mucosal Tissues
    Authors: RT Sowell, JW Goldufsky, M Rogozinska, Z Quiles, Y Cao, EF Castillo, A Finnegan, AL Marzo
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. IL-15 Enables Septic Shock by Maintaining NK Cell Integrity and Function.
    Authors: Guo Y, Luan L, Patil N, Wang J, Bohannon J, Rabacal W, Fensterheim B, Hernandez A, Sherwood E
    J Immunol, 2017;198(3):1320-1333.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Primary tumors limit metastasis formation through induction of IL15-mediated crosstalk between patrolling monocytes and NK cells
    Authors: H Kubo, S Mensurado, N Goncalves-, K Serre, B Silva-Sant
    Cancer Immunol Res, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Culture Supernates
    Applications: Bioassay
  10. IL-15 Enables Septic Shock by Maintaining NK Cell Integrity and Function
    Authors: Yin Guo
    J. Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Differential Responsiveness of Innate-like IL-17- and IFN-gamma-Producing gammadelta T Cells to Homeostatic Cytokines.
    Authors: Corpuz T, Stolp J, Kim H, Pinget G, Gray D, Cho J, Sprent J, Webster K
    J Immunol, 2016;196(2):645-54.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. Bim controls IL-15 availability and limits engagement of multiple BH3-only proteins.
    Authors: Kurtulus S, Sholl A, Toe J, Tripathi P, Raynor J, Li K, Pellegrini M, Hildeman D
    Cell Death Differ, 2015;22(1):174-84.
    Species: Mouse
    Sample Types: Whole Organism
    Applications: In vivo
  13. IRF-1 promotes liver transplant ischemia/reperfusion injury via hepatocyte IL-15/IL-15Ralpha production.
    Authors: Yokota S, Yoshida O, Dou L, Spadaro A, Isse K, Ross M, Stolz D, Kimura S, Du Q, Demetris A, Thomson A, Geller D
    J Immunol, 2015;194(12):6045-56.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. TRAF2 regulates peripheral CD8(+) T-cell and NKT-cell homeostasis by modulating sensitivity to IL-15.
    Authors: Villanueva J, Malle E, Gardam S, Silveira P, Zammit N, Walters S, Brink R, Grey S
    Eur J Immunol, 2015;45(6):1820-31.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. IL-15 Superagonist Expands mCD8+ T, NK and NKT Cells after Burn Injury but Fails to Improve Outcome during Burn Wound Infection.
    Authors: Patil N, Luan L, Bohannon J, Guo Y, Hernandez A, Fensterheim B, Sherwood E
    PLoS ONE, 2015;11(2):e0148452.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. The natural killer cell dysfunction of aged mice is due to the bone marrow stroma and is not restored by IL-15/IL-15Ralpha treatment.
    Authors: Nair S, Fang M, Sigal L
    Aging Cell, 2015;14(2):180-90.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  17. IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-gamma.
    Authors: Guo Y, Luan L, Rabacal W, Bohannon J, Fensterheim B, Hernandez A, Sherwood E
    J Immunol, 2015;195(5):2353-64.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  18. An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells.
    Authors: White A, Jenkinson W, Cowan J, Parnell S, Bacon A, Jones N, Jenkinson E, Anderson G
    , 2014;0(0):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  19. Transcription factor Runx3 regulates interleukin-15-dependent natural killer cell activation.
    Authors: Levanon, Ditsa, Negreanu, Varda, Lotem, Joseph, Bone, Karen Ra, Brenner, Ori, Leshkowitz, Dena, Groner, Yoram
    Mol Cell Biol, 2014;34(6):1158-69.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. Differential requirement for CCR4 and CCR7 during the development of innate and adaptive alphabetaT cells in the adult thymus.
    Authors: Cowan J, McCarthy N, Parnell S, White A, Bacon A, Serge A, Irla M, Lane P, Jenkinson E, Jenkinson W, Anderson G
    J Immunol, 2014;193(3):1204-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. A role for IL-15 in the migration of effector CD8 T cells to the lung airways following influenza infection.
    Authors: Verbist KC, Cole CJ, Field MB, Klonowski KD
    J. Immunol., 2011;186(1):174-82.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Bcl-2 allows effector and memory CD8+ T cells to tolerate higher expression of Bim.
    Authors: Kurtulus S, Tripathi P, Moreno-Fernandez ME
    J. Immunol., 2011;186(10):5729-37.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. Thymic and peripheral microenvironments differentially mediate development and maturation of iNKT cells by IL-15 transpresentation.
    Authors: Castillo EF, Acero LF, Stonier SW, Zhou D, Schluns KS
    Blood, 2010;116(14):2494-503.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  24. Self-specific CD8+ T cells maintain a semi-naive state following lymphopenia-induced proliferation.
    Authors: Johnson LD, Jameson SC
    J. Immunol., 2010;184(10):5604-11.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. Different routes of bacterial infection induce long-lived TH1 memory cells and short-lived TH17 cells.
    Authors: Pepper M, Linehan JL, Pagan AJ, Zell T, Dileepan T, Cleary PP, Jenkins MK
    Nat. Immunol., 2010;11(1):83-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. A defective Il15 allele underlies the deficiency in natural killer cell activity in nonobese diabetic mice.
    Authors: Suwanai H, Wilcox MA, Mathis D, Benoist C
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(20):9305-10.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  27. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
    Authors: Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW
    Blood, 2008;112(9):3704-12.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Stimulation
  28. Interleukin-15/interleukin-15R alpha complexes promote destruction of established tumors by reviving tumor-resident CD8+ T cells.
    Authors: Epardaud M, Elpek KG, Rubinstein MP, Yonekura AR, Bellemare-Pelletier A, Bronson R, Hamerman JA, Goldrath AW, Turley SJ
    Cancer Res., 2008;68(8):2972-83.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development.
    Authors: Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA
    J. Immunol., 2008;181(5):3285-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. IL-15 regulates immature B-cell homing in an Ly49D-, IL-12, and IL-18 dependent manner.
    Authors: Hart G, Avin-Wittenberg T, Shachar I
    Blood, 2007;111(1):50-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
    Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
    Blood, 2007;109(5):2234-42.
    Species: Mouse
    Sample Types: Peptide
    Applications: Surface Plasmon Resonance
  32. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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