Recombinant Mouse IL-17F Protein

Carrier Free

Catalog # Availability Size / Price Qty
2057-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2057-IL-025

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Citations (8)
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Recombinant Mouse IL-17F Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED50 for this effect is 20-120 ng/mL.
Source
E. coli-derived mouse IL-17F protein
Arg21-Ala153, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15 kDa (monomer)
SDS-PAGE
16 kDa, reducing conditions

Product Datasheets

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2057-IL (with carrier)

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2057-IL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2057-IL

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2057-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-17F

The Interleukin 17 (IL-17) family proteins, comprised of six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17F cDNA encodes a 153 amino acid (aa) protein with a putative 20 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17 sharing approximately 46% aa sequence identity. Mouse and human IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T cells and activated monocytes. Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1, 2, 5). The functions for IL‑17 RC, D, and E are not known. Purified IL-17 R and IL-17B R do not bind IL-17F with high-affinity in vitro. However, binding of IL-17F is detected in cells transfected with IL-17 R, raising the possibility that a co-receptor may be required for IL-17F signaling through IL-17 R (4). The biological activities mediated by IL-17F are similar to those of IL-17. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (3).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

Citations for Recombinant Mouse IL-17F Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. IL-1 and TNF? Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration
    Authors: H Katsura, Y Kobayashi, PR Tata, BLM Hogan
    Stem Cell Reports, 2019-03-28;0(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  2. Suppression of IL-17F, but not of IL-17A, provides protection against colitis by inducing Treg cells through modification of the intestinal microbiota
    Authors: C Tang, S Kakuta, K Shimizu, M Kadoki, T Kamiya, T Shimazu, S Kubo, S Saijo, H Ishigame, S Nakae, Y Iwakura
    Nat. Immunol., 2018-06-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. T-Lymphocytes Enable Osteoblast Maturation via IL-17F during the Early Phase of Fracture Repair.
    Authors: Nam D, Mau E, Wang Y, Wright D, Silkstone D, Whetstone H, Whyne C, Alman B
    PLoS ONE, 2012-06-29;7(6):e40044.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness.
    Authors: Wilson RH, Whitehead GS, Nakano H, Free ME, Kolls JK, Cook DN
    Am. J. Respir. Crit. Care Med., 2009-08-06;180(8):720-30.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.
    Authors: Ishigame H, Kakuta S, Nagai T, Kadoki M, Nambu A, Komiyama Y, Fujikado N, Tanahashi Y, Akitsu A, Kotaki H, Sudo K, Nakae S, Sasakawa C, Iwakura Y
    Immunity, 2009-01-16;30(1):108-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Requirement of IL-17RA in Con A induced hepatitis and negative regulation of IL-17 production in mouse T cells.
    Authors: Nagata T, McKinley L, Peschon JJ, Alcorn JF, Aujla SJ, Kolls JK
    J. Immunol., 2008-12-01;181(11):7473-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis.
    Authors: Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M
    J. Invest. Dermatol., 2008-10-02;129(3):650-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008-09-15;181(6):4299-310.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo

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